Yoh Sakuma

TL;DR: Overexpression of the DREB1A cDNA in transgenic Arabidopsis plants not only induced strong expression of the target genes under unstressed conditions but also caused dwarfed phenotypes in the transgenic plants, and revealed freezing and dehydration tolerance.

TL;DR: Gel mobility shift assay using mutant DREB proteins showed that the two amino acids, valine and glutamic acid conserved in the ERF/AP2 domains, especially valine, have important roles in DNA-binding specificity.

TL;DR: OsDREB1A is potentially useful for producing transgenic monocots that are tolerant to drought, high-salt, and/or cold stresses and has functional similarity to DREB 1A, however, in microarray and RNA blot analyses, some stress-inducible target genes of the DREb1A proteins that have only ACCGAC as DRE were not over-expressed in the OsDRE B1A transgenic Arabidopsis.

TL;DR: The complete NAC recognition sequence, containing CATGT and harboring CACG as the core DNA binding site is determined, which indicates that other interacting factors may be necessary for the induction of erd1 in Arabidopsis under stress conditions.

TL;DR: DREB2A domain analysis using Arabidopsis protoplasts identified a transcriptional activation domain between residues 254 and 335, and deletion of a region between residues 136 and 165 plays a role in the stability of this protein in the nucleus, which is important for protein activation.