Yongzhong Li

TL;DR: Using a thermal polymerization approach and polymerizable pH and oxygen sensing monomers with green and red emission spectra, respectively, new pH, oxygen, and their dual sensing membranes were prepared using poly(2-hydroxyethyl methacrylate)-co-poly(acrylamide) as a matrix, accounting in part for the sensors' biocompatibility without apparent toxicity to HeLa cells after 40 hours incubation.

TL;DR: An improved reverse-transcriptase polymerase chain reaction (RT-PCR) protocol is reported which allows the direct measurement of gene expression in one tube at the single mammalian cell level, and is based on intercalator-based (SYBR Green PCR) chemistry, which is less expensive and suitable for high-throughput platforms.

TL;DR: The dramatic difference of cell uptake between P5 and P7 indicated the cationic amino groups had a strong influence on the cell internalization to enhance the endocytosis pathway.

TL;DR: This is the first reported application of a hydrophobic 2,1,3-benzothiadiazole-containing 2PA red emitter delivered into the cytoplasm of cells for bioimaging and toxicity assessment.

TL;DR: In this article, the authors present an automated, high-throughput, multi-parameter integrated analysis system to monitor, in parallel, cellular parameters descriptive of cell function and associated physiology both singly and in interactions with other cells in tissues.