Dually fluorescent sensing of pH and dissolved oxygen using a membrane made from polymerizable sensing monomers
Yanqing Tian,Bradley R. Shumway,A. Cody Youngbull,Yongzhong Li,Alex K.-Y. Jen,Roger H. Johnson,Deirdre R. Meldrum +6 more
Reads0
Chats0
TLDR
Using a thermal polymerization approach and polymerizable pH and oxygen sensing monomers with green and red emission spectra, respectively, new pH, oxygen, and their dual sensing membranes were prepared using poly(2-hydroxyethyl methacrylate)-co-poly(acrylamide) as a matrix, accounting in part for the sensors' biocompatibility without apparent toxicity to HeLa cells after 40 hours incubation.Abstract:
Using a thermal polymerization approach and polymerizable pH and oxygen sensing monomers with green and red emission spectra, respectively, new pH, oxygen, and their dual-sensing membranes were prepared using poly(2-hydroxyethyl methacrylate)-co-poly(acrylamide) as a matrix. The sensors were grafted on acrylate-modified quartz glass and characterized under different pH values, oxygen concentrations, ion strengths, temperatures and cell culture media. The pH and oxygen sensors were excited using the same excitation wavelength and exhibited well-separated emission spectra. The pH sensing films showed good response over the pH range 5.5–8.5, corresponding to pKa values in the biologically relevant range between 6.9 and 7.1. The oxygen sensing films exhibited linear Stern–Volmer quenching responses to dissolved oxygen. As the sensing membranes were prepared using thermally initiated polymerization of sensing moiety-containing monomers, no leaching of the sensors from the membranes to buffers or medium was observed. This advantageous characteristic accounts in part for the sensors’ biocompatibility without apparent toxicity to HeLa cells after 40 h incubation. The dual-sensing membrane was used to measure pH and dissolved oxygen simultaneously. The measured results correlated with the set-point values.read more
Citations
More filters
Journal ArticleDOI
Optical methods for sensing and imaging oxygen: materials, spectroscopies and applications
Xu-dong Wang,Otto S. Wolfbeis +1 more
TL;DR: The current state of optical methods for sensing oxygen have become powerful alternatives to electrochemical detection and in the process of replacing the Clark electrode in many fields and a selection of specific applications of such sensors are given.
Journal ArticleDOI
Multiple fluorescent chemical sensing and imaging
TL;DR: This critical review discusses in this critical review the state of the art in terms of spectroscopic principles, materials, and selected examples for dual and triple sensors along with a look into the future.
Journal ArticleDOI
Optical Sensing and Imaging of pH Values: Spectroscopies, Materials, and Applications
TL;DR: This is the first comprehensive review on methods and materials for use in optical sensing of pH values and on applications of such sensors.
Journal ArticleDOI
Ratiometric optical oxygen sensing: a review in respect of material design
TL;DR: This review describes the overall progress made in the past ten years on ratiometric optical ground-state triplet oxygen sensing and offers a critical comparison of various methods reported in the literature and provides a development blueprint for ratiometry optical oxygen sensing.
References
More filters
BookDOI
Molecular fluorescence : principles and applications
TL;DR: In this article, the effects of intermolecular photophysical processes on fluorescence emission are discussed and an analysis of the effect of polarity of fluorescence emissions is presented.
Journal ArticleDOI
A water-soluble cationic oligopyrene derivative : Spectroscopic studies and sensing applications
TL;DR: A water-soluble cationic conjugated oligomer, oligo(2-(4-(1-pyrenyl)butanoyloxy)ethyltrimethylammonium bromide) (OPBEAB) was synthesized by the combination of chemical and electrochemical synthesis techniques.
Journal ArticleDOI
Materials for fluorescence-based optical chemical sensors
TL;DR: In this article, the progress made in the past years and some of the future aspects and challenges are discussed, as well as the potential of using fluorescence in the future. But the implementation of spectroscopic schemes into a useful sensing scheme has been hampered by the lack of appropriate materials including polymers and particles, indicator probes, molecular receptors, carriers, catalysts and fluorescent semiconductor materials.
Journal ArticleDOI
Bioinspired Molecular Design of Light‐Harvesting Multiporphyrin Arrays
TL;DR: Dendritic multiporphyrin arrays have been proven to be promising candidates for both providing a large absorption cross-section and enabling the vectorial transfer of energy over a long distance to a designated point.
Journal ArticleDOI
A real-time ratiometric method for the determination of molecular oxygen inside living cells using sol-gel-based spherical optical nanosensors with applications to rat C6 glioma.
TL;DR: The firstsol-gel-based, ratiometric, optical nanosensors, or sol-gel probes encapsulated by biologically localized embedding (PEBBLEs), are made and demonstrated here to enable reliable, real-time measurements of subcellular molecular oxygen.
Related Papers (5)
Optical multiple chemical sensing : status and current challenges
Stefan Nagl,Otto S. Wolfbeis +1 more