The lion's share of bacteria in various environments cannot be cloned in the laboratory and thus cannot be sequenced using existing technologies. A major goal of single-cell genomics is to complement gene-centric metagenomic data with whole-genome assemblies of uncultivated organisms. Assembly of single-cell data is challenging because of highly non-uniform read coverage as well as elevated levels of sequencing errors and chimeric reads. We describe SPAdes, a new assembler for both single-cell and standard (multicell) assembly, and demonstrate that it improves on the recently released E+V-SC assembler (specialized for single-cell data) and on popular assemblers Velvet and SoapDeNovo (for multicell data). SPAdes generates single-cell assemblies, providing information about genomes of uncultivatable bacteria that vastly exceeds what may be obtained via traditional metagenomics studies. SPAdes is available online ( http://bioinf.spbau.ru/spades ). It is distributed as open source software.

SPAdes, a new genome assembly algorithm and its applications to single-cell sequencing ( 7th Annual SFAF Meeting, 2012)

CA : A Cancer Journal for Clinicians

We investigate conditions sufficient for identification of average treatment effects using instrumental variables. First we show that the existence of valid instruments is not sufficient to identify any meaningful average treatment effect. We then establish that the combination of an instrument and a condition on the relation between the instrument and the participation status is sufficient for identification of a local average treatment effect for those who can be induced to change their participation status by changing the value of the instrument. Finally we derive the probability limit of the standard IV estimator under these conditions. It is seen to be a weighted average of local average treatment effects.

Evolution and Rationality Some Recent Game-Theoretic Results. Identification and Estimation of Local Average Treatment Effects

日本物理学会誌及びJournal of the Physical Society of Japanの月刊について

The mechanistic target of rapamycin (mTOR) coordinates eukaryotic cell growth and metabolism with environmental inputs, including nutrients and growth factors. Extensive research over the past two decades has established a central role for mTOR in regulating many fundamental cell processes, from protein synthesis to autophagy, and deregulated mTOR signaling is implicated in the progression of cancer and diabetes, as well as the aging process. Here, we review recent advances in our understanding of mTOR function, regulation, and importance in mammalian physiology. We also highlight how the mTOR signaling network contributes to human disease and discuss the current and future prospects for therapeutically targeting mTOR in the clinic.

mTOR Signaling in Growth, Metabolism, and Disease

Large eddy simulation was applied to simulate the compressible flow past multiple wall-mounted rectangular cylinders in a channel flow. The dynamic sub-grid stress model was employed to approximate the sub-grid scale effects. For flow past single wall-mounted cylinder, our calculated results agree well with the results of both previous experimental and numerical results which showed the formation and diffusion of vortexes around the cylinder. Flow past two wall-mounted cylinders has also been simulated numerically and our numerical results disclosed the formation of the vortex street behind the second cylinder and the developing process of flow field, which is important for practical engineering application.

Large Eddy Simulation of a 3d Channel Flow with Wall-Mounted Rectangular Cylinders

The transformation mechanisms of vortex structures on vortex-induced vibration of an elastically mounted sphere by Lorentz force

Stakeholder requirement evaluation of smart industrial service ecosystem under Pythagorean fuzzy environment for complex industrial contexts: A case study of renewable energy park

The invention discloses a rotary power exchanger with an extended inflow angle structure, and belongs to the technical field of fluid residual pressure recycling. The exchanger comprises a rotor, an upper stator and a lower stator which are positioned at the two ends of the rotor, and a rotor sleeve, wherein the stators are provided with inflow liquid collecting slots with extended angle structures, so that pressure gradient is remarkably reduced when speed is in gradient change in a switching process among a high-pressure communication region, a sealing region and a low-pressure communication region of a duct, and therefore, jet-flow mixing of the liquid can be effectively reduced. The rotary power exchanger disclosed by the invention is reasonable in inflow structure design, simple and convenient to manufacture, and capable of effectively restraining jet-flow mixing in the duct and guaranteeing concentration quality of a first liquid pressurized in a pressure energy transmission process, so that a concentration value at a high-pressure outlet is within a stable range, and the efficient operation of the power exchanger and a membrane system is guaranteed.

Zhihua Chen

Papers

Large Eddy Simulation of a 3d Channel Flow with Wall-Mounted Rectangular Cylinders

The transformation mechanisms of vortex structures on vortex-induced vibration of an elastically mounted sphere by Lorentz force

Stakeholder requirement evaluation of smart industrial service ecosystem under Pythagorean fuzzy environment for complex industrial contexts: A case study of renewable energy park

Rotary power exchanger with extended inflow angle structure

Experimental Research on The Mechanism of Lift Amplification and Vibration Suppression of Lorentz Force