Z
Zoi Lygerou
Researcher at University of Patras
Publications - 99
Citations - 4975
Zoi Lygerou is an academic researcher from University of Patras. The author has contributed to research in topics: DNA replication factor CDT1 & Geminin. The author has an hindex of 34, co-authored 86 publications receiving 4469 citations. Previous affiliations of Zoi Lygerou include European Bioinformatics Institute.
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Journal ArticleDOI
The Cdt1 protein is required to license DNA for replication in fission yeast.
TL;DR: Genes related to Cdt1 have been found in Metazoa and plants, suggesting that the cooperation of Cdc6/Cdc18 with Cdt 1 to load MCM proteins onto chromatin may be a generally conserved feature of DNA licensing in eukaryotes.
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Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis.
Hideo Nishitani,Nozomi Sugimoto,Vassilis Roukos,Yohsuke Nakanishi,Masafumi Saijo,Chikashi Obuse,Toshiki Tsurimoto,Keiichi I. Nakayama,Keiko Nakayama,Masatoshi Fujita,Zoi Lygerou,Takeharu Nishimoto +11 more
TL;DR: It is shown here that the N‐terminal 100 amino acids of human Cdt1 are recognized for proteolysis by two distinct E3 ubiquitin ligases during S–G2 phases, which are essential for DDB1‐Cul4‐mediated proteolyses and following ultraviolet‐irradiation.
Journal ArticleDOI
Control of DNA replication licensing in a cell cycle
Hideo Nishitani,Zoi Lygerou +1 more
TL;DR: Geminin, whose degradation at the end of mitosis is essential for a new round of licensing, has been shown to bind Cdt1 and negatively regulate it, providing a new insight into the regulation of DNA replication in higher eukaryotes.
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Accurate Processing of a Eukaryotic Precursor Ribosomal RNA by Ribonuclease MRP in Vitro
TL;DR: Biochemical purification and the use of extracts depleted of the MRP RNA demonstrate that endonucleolytic cleavage of the pre-rRNA is directly mediated by RNase MRP, establishing a role for RNaseMRP in the nucleolus.
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The human licensing factor for DNA replication Cdt1 accumulates in G1 and is destabilized after initiation of S-phase.
TL;DR: It is suggested that the presence of active hCdt1 may be crucial for determining when licensing is legitimate in human cells, and proteolytic rather than transcriptional controls ensure the timely accumulation of hCdit1.