Akita Prefectural University

About: Akita Prefectural University is a education organization based out in Akita, Japan. It is known for research contribution in the topics: Magnetic field & Starch. The organization has 1631 authors who have published 3107 publications receiving 45764 citations. The organization is also known as: Akita kenritsu daigaku.

Highly Sensitive Strain Sensor Based on a Stretchable and Conductive Poly(vinyl alcohol)/Phytic Acid/NH2-POSS Hydrogel with a 3D Microporous Structure.

Abstract: Conductive hydrogel-based wearable strain sensors with tough, stretchable, self-recoverable, and highly sensitive properties are highly demanded for applications in electronic skin and human-machine interface. However, currently, hydrogel-based strain sensors put forward higher requirements on their biocompatibility, mechanical strength, and sensitivity. Herein, we report a poly(vinyl alcohol)/phytic acid/amino-polyhedral oligomeric silsesquioxane (PVA/PA/NH2-POSS) conductive composite hydrogel prepared via a facile freeze-thaw cycle method. Within this hydrogel, PA acts as a cross-linking agent and ionizes hydrogen ions to endow the material with ionic conductivity, while NH2-POSS acts as a second cross-linking agent by increasing the cross-linking density of the three-dimensional network structure. The effect of the content of NH2-POSS is investigated, and the composite hydrogel with 2 wt % NH2-POSS displays a uniform and dense three-dimensional (3D) network microporous structure, high conductivity of 2.41 S/m, and tensile strength and elongation at break of 361 kPa and 363%, respectively. This hydrogel is biocompatible and has demonstrated the application as a strain sensor monitoring different human movements. The assembled sensor is stretchable, self-recoverable, and highly sensitive with fast response time (220 ms) and excellent sensitivity (GF = 3.44).

Low aquaporin content and low osmotic water permeability of the plasma and vacuolar membranes of a CAM plant Graptopetalum paraguayense: comparison with radish.

Abstract: Aquaporin facilitates the osmotic water transport across biomembranes and is involved in the transcellular and intracellular water flow in plants. We immunochemically quantified the aquaporin level in leaf plasma membranes (PM) and tonoplast of Graptopetalum paraguayense, a Crassulacean acid metabolism (CAM) plant. The aquaporin content in the Graptopetalum tonoplast was approximately 1% of that of radish. The content was calculated to be about 3 microg mg(-1) of tonoplast protein. The level of PM aquaporin in Graptopetalum was determined to be less than 20% of that of radish, in which an aquaporin was a major protein of the PM. The PM aquaporin was detected in the mesophyll tissue of Graptopetalum leaf by tissue print immunoblotting. The osmotic water permeability of PM and tonoplast vesicles prepared from both plants was determined with a stopped-flow spectrophotometer. The water permeability of PM was lower than that of the tonoplast in both plants. The Graptopetalum PM vesicles hardly showed water permeability, although the tonoplast showed a relatively high permeability. The water permeability changed depending on the assay temperature and was also partially inhibited by a sulfhydryl reagent. Furthermore, measurement of the rate of swelling and shrinking in different mannitol concentrations revealed that the protoplasts of Graptopetalum showed low water permeability. These results suggest that the low content of aquaporins in PM and tonoplast is one of the causes of the low water permeability of GRAPTOPETALUM: The relationship between the water-storage function of succulent leaves of CAM plants and the low aquaporin level is also discussed.

The effects of Sasa and canopy gap formation on tree regeneration in an old beech forest

Abstract: We examined the response of tree seedling emergence and survival to the dieback of Sasa and canopy gap formation in an old-growth forest near Lake Towada, northern Japan. Synchronous death of Sasa occurred in 1995. We established four types of sampling sites differing in forest canopy conditions (Closed or Gap) and Sasa status (Dead or Live). Gap-Dead sites had the highest light levels and the greatest fluctuation in soil temperatures. The death of Sasa alone facilitated the emergence (Acer japonicum, Fagus crenata, Fraxinus lanuginosa, and Tilia japonica) and survival (Acanthopanax sciadophylloides, F. crenata, F. lanuginosa, Kalopanax pictus, and Sorbus commixta) of species with a seedling bank strategy. Cercidiphyllum japonicum grew at all sites at a higher density than other species, but survived well only in Gap-Dead sites. This behaviour was associated with a seed rain strategy. The additive effects of Sasa death and canopy gap formation promoted seedling emergence of pioneer tree species (...

Reliability of thick Al wire bonds in IGBT modules for traction motor drives

Abstract: Reliability enhancement of thick Al wire bonds during thermal fatigue test has been investigated from a metallurgical viewpoint. Al wire bonds degrade with the increase of crack length during thermal fatigue tests with high /spl Delta/T/sub j/ due to the tensile stress generated by the thermal expansion coefficient mismatch between Al wires and Si. It is also found that cracks propagate along the small grain boundaries of Al wires at the bonding interface. It is predicted that the Al wire bonds may not degrade due to thermal fatigue if /spl Delta/T/sub j/ is controlled below 40 K, i.e., keeping it within the actual temperature fluctuation range in IGBT modules for traction motor drives. The reliability of Al wire bonds can be enhanced by increasing the grain size of the Al wire at the bonding interface. The high temperature bonding is considered to be a good candidate for enhancing the reliability of Al wire bonds.

Structural and enzymatic characterization of the isoamylase1 homo-oligomer and the isoamylase1–isoamylase2 hetero-oligomer from rice endosperm

Abstract: The present study established that there are two distinct polymeric forms of isoamylase1 (ISA1) in rice endosperm: presumably a homo-pentamer of ISA1 and a hetero-hexamer composed of five ISA1 and one ISA2. The molecular sizes of the homo- and hetero-oligomers, which could be fractionated by hydrophobic chromatography, were approximately 420-480 and 510-550 kDa, respectively. The hetero-oligomer exhibited higher affinities for various branched polyglucans, especially for phytoglycogen, which had a K(m) value that was approximately 12 times lower relative to that with the homo-oligomer, although no marked differences were found in chain preferences for debranching of amylopectin and phytoglycogen between these forms. The hetero-oligomer was active even when incubated at 50 degrees C for 10 min, while the homo-multimer was completely inactivated at 40 degrees C in 10 min. When the ISA1 homo-oligomer was incubated with the ISA2 protein expressed in Escherichia coli and applied onto a nondenature polyacrylamide gel, additional debranching activity bands which were specific for the purified ISA1-ISA2 preparation were also detected, indicating that ISA1 and ISA2 combine to form a hetero-oligomer. These results suggest that the hetero-oligomer plays a predominant role in the amylopectin biosynthesis in rice endosperm although the homo-oligomer can complement the function of the hetero-oligomer at least to some extent.