Showing papers by "Aoyama Gakuin University published in 1983"
TL;DR: Two low-molecular-weight thiol proteinase inhibitors were purified from newborn rat epidermis by homogenization and extraction at 37 degrees C, suggesting some important roles of the beta turn of both inhibitors in the interaction with the thiol enzymes.
Abstract: Two low-molecular-weight thiol proteinase inhibitors, TPI(1) and TPI(2), were purified from newborn rat epidermis by homogenization and extraction at 37 degrees C, followed by lyophilization, Sephadex G-75 gel filtration and DEAE-cellulose column chromatography. Both purified inhibitors were shown to be homogeneous by polyacrylamide gel electrophoresis in the presence and absence of SDS. TPI(1) was more anionic than TPI(2), and the molecular weights of TPI(1) and TPI(2) were determined to be ca. 12,000 and 13,000, respectively. They were highly specific for thiol proteinase; TPI(1) was especially more effective than TPI(2) on the proteolytic activity of casein and hydrolytic activity of papain on authentic substrates, and a similar but lesser effect was detected for ficin. They inhibited the enzyme in a noncompetitive manner with inhibition constants of 6.01 X 10(-8) M and 4.57 X 10(-7) M for TPI(1) and TPI(2), respectively, calculated from the BAPA hydrolytic activity of papain. Both inhibitors were quite stable proteins as to heat and extreme pHs. The amino acid composition of TPI(1) and TPI(2) were slightly different from each other, and both inhibitors contained no cysteinyl or cytinyl residue and trptophanyl residue. From these results, TPI(1) and TPI(2) seem to be iso-inhibitors. The absorption and CD spectra of TPI(1) and TPI(2) in the near ultraviolet region indicated that the conformation in the vicinity of aromatic amino acid residues in TPI(1) was slightly different from that of TPI(2). The CD spectra of both inhibitors in the far ultraviolet region showed the presence of the beta turn structure in the protein moiety, suggesting some important roles of the beta turn of both inhibitors in the interaction with the thiol enzymes.
24 citations
TL;DR: In this paper, the reaction of 4,6-0-benzylidene-1,2-dideoxy-3,0-mesyl-D-ribo-hex-1-enopyranose reacted with 2-propenylmagnesium chloride or 2-methyl-2-pro-penyl magnetium chloride.
15 citations
TL;DR: In this article, the two-dimensional magnetic ordering of Lu 2 Fe 3 O 7 was studied by Mossbauer and neutron diffraction experiments, and the authors concluded that the Mossbau spectrum shows two kinds of ordering temperatures, 260 and 60 K, but neutron diffusion measurement shows the ordering below 300 K without any anomaly.
14 citations
TL;DR: The results indicate that the conformational changes and dissociation process of catalase into subunits can be monitored spectrophotometrically in relation to enzyme activity, and that subtle conformations near the heme groups and polypeptide backbone play an important role in maintaining full enzyme activity of the catal enzyme molecule.
Abstract: The dissociation of porcine erythrocyte catalase [EC 1.11.1.6] into subunits on denaturation with alkali, GuHCl and urea was investigated by following the changes in hydrodynamic properties, absorption and CD spectra in the Soret region and inactivation of the enzyme. It was found that dissociation proceeded in an "all or none" manner from the native tetramer (molecular weight, ca. 250,000) into identical 1/4-sized monomers (molecular weight, ca. 54,000 with alkali, 65,000 with urea and 71,000 with GuHCl) as estimated by ultracentrifugal analyses. On this dissociation, the sedimentation coefficient decreased from about 11S to 5.1 - 3.7S, and absorption spectra in the Soret region decreased to about 40% of the native level and showed a broad band around 365-375 nm and a shoulder around 415-420 nm; these changes were accompanied by complete loss of enzyme activity. The change in enzyme activity correlated well with that of absorption and CD spectra in the Soret region, depending on denaturation time, alkaline pH used and concentration of both denaturants. The reassociated catalase obtained by removing urea by dialysis was characterized by recovery of distinct CD bands in the Soret and near ultraviolet regions, although the partial refolding of alpha-helical conformation occurred without recovery of enzyme activity. These results indicate that the conformational changes and dissociation process of catalase into subunits can be monitored spectrophotometrically in relation to enzyme activity, and that subtle conformations near the heme groups and polypeptide backbone play an important role in maintaining full enzyme activity of the catalase molecule.
13 citations
TL;DR: In this article, the magnetic structure of non-stoichiometric Fe 1− x O was investigated by neutron scattering measurements on a quenched single crystal of Fe 0.91 O below the Neel temperature.
11 citations
TL;DR: In this article, the authors focus on three important determinants of economic growth in the Chinese context: the motivation of economic units, the absorption of foreign technology and its diffusion and agricultural productivity, and the comprehensiveness of economic reform likely in China means that it will only be possible to judge it over a time horizon longer than five years.
9 citations
TL;DR: It has been shown that a similarity in geometrical shape of interacting groups is responsible for specific molecular recognition of acylurea derivatives.
Abstract: For the oxidation of associating thiols [(1) and (2)] and the formation of a 1 : 1 crystalline complex between acylurea derivatives [(6) and (7)] it has been shown that a similarity in geometrical shape of interacting groups is responsible for specific molecular recognition.
7 citations
TL;DR: In this article, the lead concentration and 210Pb activity profiles of 8 core samples from the inner Tokyo Bay were determined and the lead profiles showed a typical lead pollution pattern by human activities.
Abstract: Lead concentration and 210Pb activity profiles of 8 core samples from the inner Tokyo Bay were determined. The lead profiles showed a typical lead pollution pattern by human activities. Generally the lead concentration increased gradually from the background level of about 15 ppm before 1900 A.D. to the level in 1940's and then grew up rapidly until 1970. The pollution lead went up as high as about 7 times the natural value in around 1970 in some cores. Since 1970, lead concentration has been decreasing clearly up to date. The average lead accumulation rates in the bay were estimated to be 7.1μg/cm2/y in 1980 and 13μg/cm2/y in 1970. The value of 2.2μg/cm2/y in around 1880 seems close to the natural input rate. More than 75% of the accumulated lead during 1945–1980 was contributed by the pollution lead. Even before that time, 1900–1945, a half of the accumulated lead resulted from the human activities. The values indicate that the Tokyo Bay has been exposed to severe lead pollution for a long time.
7 citations
TL;DR: In this paper, the production de pions multiples a partir de l'observation de rayons gamma produits dans la chambre a emulsion exposee a Chacaltaya, Bolivie, a 5200 m au-dessus de mer.
Abstract: On etudie la production de pions multiples a partir de l'observation de rayons gamma produits dans la chambre a emulsion exposee a Chacaltaya, Bolivie, a 5200 m au-dessus de mer. On compile 80 evenements avec ∑Ej>20TeV. On discute des implications possibles sur les modeles de boule de feu
6 citations
TL;DR: In this paper, the author's (Awaya) method is applied to a two-dimensional curve fitting to the generated data with normal and Poisson errors in abscissa and ordinate, respectively.
6 citations
TL;DR: Magnetization and neutron diffraction studies were carried out on polycrystalline RFeMO 4, especially on YFeMnO 4, where 2-dimensional spin ordering develops below 140 K, whereas a small ferromagnetic moment is induced by field cooling below 30 K as discussed by the authors.
TL;DR: In this paper, the effect of ferrous ions on magnetocrystalline anisotropy constants on monocrystals of the Mn-Zn-Fe ternary ferrite system was studied.
TL;DR: In this paper, the two-dimensional electron momentum densities in Nb-Mo alloys were calculated on the basis of the average t -matrix approximation scheme, which was then transformed into the reduced k -space by means of the procedure proposed by Lock et al.
Abstract: The two-dimensional electron momentum densities in Nb–Mo alloys are calculated on the basis of the average t -matrix approximation scheme. The momentum densities obtained are transformed into the reduced k -space by means of the procedure proposed by Lock et at. Our results agree qualitatively with the experimental electron-positron pair momentum densities obtained from two-dimensional angular correlation of positron annihilation radiation.
TL;DR: The relationship between the recognition of shapes and the electromyogram (e.m.m.) generated in the human on the surface of the muscle involved is discussed.
Abstract: Many studies related to human sensation have been made from the viewpoints of engineering, medical science and psychology. The problem of the quantification of sensation is of great importance and cannot be ignored, although only a few papers on this subject have appeared. This paper discusses the relationship between the recognition of shapes and the electromyogram (e.m.g.) generated in the human on the surface of the muscle involved. To observe the e.m.g., the subject was placed inside a shielded room with Ag−AgCl electrodes located at two points on the skin. The two electrodes were spaced 50 mm apart, but variations of the separation in the range 5–7 mm had no effects on the e.m.g. Three spectral peaks (100, 160, 250 Hz) for the response were obtained for each shape (sphere, tetrahedron, cube). The frequencies corresponding with the peak spectral values in the response are the same, and are independent of the forms. Shapes can therefore be characterised by the spectral forms of the forms.
TL;DR: The distribution of 137C in volcanic rocks was measured for sliced samples of solid lava blocks from Izu-Oshima island, Japan as mentioned in this paper, and the decrease in 137C concentration with the increase in depth, together with the uneven spatial distribution, suggested that the fallout was responsible for the occurrence of 137Cs and that 137Cs might have penetrated into lavas as deep as several centimeters.
Abstract: The distribution of 137Cs in volcanic rocks was measured for sliced samples of solid lava blocks from Izu-Oshima island, Japan. The decrease in 137Cs concentration with the increase in depth, together with the uneven spatial distribution, suggested that the fallout was responsible for the occurrence of 137Cs and that 137Cs might have penetrated into lavas as deep as several centimeters.
TL;DR: In this paper, the role of the exchange interaction between electrons in the screening of the ion-ion interaction in a metal was investigated and it was shown that the magnetic properties of the metal are reflected on the elastic properties such as phonon frequency and sound velocity.
Abstract: By pursuing the role of the exchange interaction between electrons in the screening of the ion-ion interaction in a metal, we recently showed how directly the magnetic properties of the metal are reflected on the elastic properties such as phonon frequency and sound velocity. Now we ask the opposite question: Is it necessary to consider the electron-phonon interaction (EPI) in understanding the magnetic properties of a metal? Indeed we find it essential to consider EPI; by the effect of EPI, in the paramagnetic state of an itenerant electron ferromagnet the spin susceptibility is made Curie-Weiss-like, and in the ferromagnetic state the magnetization can be reduced by as much as ∼1μB per atom. These findings offer a comprehensive fundamental view point to discuss the relation between the elastic and magnetic properties in itinerant electron ferromagnets.
TL;DR: In this article, the spin polarization of tunnelling electrons from Ni1−xPtx and Ni1 −xPdx was measured in a lower concentration range of Pt and Pd, and the observed spin polarization was not proportional to the bulk magnetization.
TL;DR: In this paper, the effect of the electron-phonon interaction on the magnetic properties of an itinerant electron ferromagnet was investigated and it was shown that the electronphon interaction can make the paramagnetic spin susceptibility Curie-Weiss like, with a Curie temperature much lower than in the Stoner theory.