Showing papers by "Catholic University of the Sacred Heart published in 1969"

Effect of pH and CO2 concentration changes on lipids and fatty acids of Saccharomyces cerevisiae.

Abstract: The lipid composition of S. cerevisiae cells was studied in different conditions of pH and of HCO3- and CO2 concentration.

Submicroscopic organization of the otic ganglion of the adult rabbit.

Abstract: The ultrastructural organization of the otic ganglion of the adult rabbit has been examined. In addition to a description of the basic characteristics of the neuron and its associated satellite cell, there is presented evidence that suggests an intensive metabolic relationship between these two cell types. This evidence consists of the presence of numerous pinocytotic vesicles in the satellite cell cytoplasm near the neuron and the frequent existence of junctional complexes between nerve and satellite cell. Also noted was the occurrence of two types of neurons somewhat similar to the “clear” and “dark” cells previously described. These two cell types are distinguishable on the basis of the organization and amount of endoplasmic reticulum. The great majority of nerve endings are axodendritic in contrast to the exclusive presence of axosomatic terminations described in chick ciliary ganglion. Axodendritic contacts were found between preganglionic axons and medium and especially small dendritic branches.

The biosynthesis of triphosphoinositide by purified myelin of peripheral nerve

Abstract: — The distribution of diphosphoinositide kinase activity in homogenates and myelin of rabbit nerve was determined by measuring the synthesis of labelled triphosphoinositide. Evidence is presented to show that the biosynthesis of triphosphoinositide in peripheral myelin involves a membrane-bound diphosphoinositide kinase.

Differenze Ultrastrutturali Di Alcune Specie Di Trebouxia Poste in Condizioni Di Illuminazione Differenti

Abstract: Ultrastructural changes in some species of « Trebouxia » under different light conditions. — Some species of the phycobiont alga Trebouxia (Tr. decolorans and Tr. albulescens), both isolated and grown on synthetic medium and still in the lichen, were examined in order to study the effect of light on the plastid ultrastructures. The species isolated from Buellia punctata and Xanthoria parietina were very sensitive to light condition and lost their chlorophyll content quickly. Striking ultrastructural changes were found in the algae grown under small light intensities and those which become achloric owing to strong light. In the latter, modifications of the Iamellar System were observed. The disappearance of Chlorophyll pigments was followed by a reduced electron density of the whole Iamellar system, as if were lacking the Iipidic compounds which are usually present and absorb fixators and dyers, thus allowing a good view. On the contrary, normal light conditions did not affect cultures of Trebouxi...

Synthesis of adenosine triphosphate by myelin of spinal nerves of rabbit.

Abstract: Abstract— 1 The myelin fraction isolated by isopycnic gradient centrifugation from rabbit nerve is able to synthesize ATP at substrate level through the Embden‐Meyerhof pathway. Suitable conditions are described to preserve the association of glycolytic enzymes with isolated myelin. 2 Except for phosphofructokinase and ketose‐1‐phosphate aldolase, all the remaining glycolytic enzymes are present in the myelin. A wide divergence was found in the firmness of the association of individual glycolytic enzymes with myelin under the condition of isolation; some, like glucosephosphate isomerase and glyceraldehydephosphate dehydrogenase were retained in high percentage (about 60 per cent of the activity of the homogenate is myelin‐bound); others were weakly bound (no more than 7–6 percent of the lactate dehydrogenase activity of the homogenate is myelin‐bound). 3 By using glyceraldehyde‐3‐phosphate as substrate for glycolysis, about 25 per cent of the total glycolytic activity of rabbit‐nerve homogenate is associated with the myelin. 4 Glucosephosphate isomerase and lactate dehydrogenase may be extracted from and readily recombined with the myelin.

The histochemical detection of thallium in paraffin-embedded tissues by the sulfide- selenium-silver method

Abstract: Thallium can be histochemically localized in formalin-fixed, paraffin-processed tissues by treating sections, after passing them through xylene and graded alcohols to water, with gaseous H2S for 15 min and with 20% ammonium sulfide saturated with powdered selenium for 10 min. Sections are then washed, treated 10 min with 20% H2O2, and incubated in darkness for 20-30 min in the following mixture: 25% gum acacia, 10 ml; 2% hydroquinone in 5% citric acid, 1 ml; and 10% AgNO3, 0.1 ml. Tissues and cells, which contain thallium, are demonstrated by small black granules of silver.

Problems of the Underdeveloped Regions of Italy

Abstract: Interregional differences in the standard of living are a common feature of all nations, even the most industrialised. Historical experience shows that in the latter they are less pronounced than in underdeveloped countries. They are at the same time less acutely felt and less likely to arouse political pressures for the obvious reason that the per capita income of the backward regions in an advanced economy, though lower as compared with more advanced areas or the national average, is nonetheless sufficient, in absolute terms, to assure a satisfactory standard of living.

Interaction Between Red Cell Stroma and Streptolysin O: Inhibition of Cytotoxic Activity

Abstract: A blood red cell stroma extract neutralizes cytotoxic activity of streptolysin O. The component responsible for inhibition was precipitated by 60% ammonium sulfate and by lysozyme.

Denervated taste buds. I. General ultrastructural observations.

Abstract: La denervazione dei calici gustativi provoca due tipi fondamentali di alterazioni cellulari: il primo e rappresentato da lisosomi e citosegregosomi che colpiscono le cellule scarsamento differenziate, il secondo e invece rappresentato da lesioni che sconvolgono l'intera organizzazione citoplasmatica delle cellule di II tipo.