Forensic Science Service

About: Forensic Science Service is a based out in . It is known for research contribution in the topics: Population & DNA profiling. The organization has 573 authors who have published 599 publications receiving 27149 citations.

[Genetic polymorphisms of 16 non-CODIS STR loci in Beijing Han population].

Abstract: OBJECTIVE To investigate the genetic polymorphisms of 16 non-CODIS loci (D6S477, D22-GATA198B05, D15S659, D8S1132, D3S3045, D17S1290, D14S608, D2S441, D18S535, D13S325, D10S1435, DlS2368, DIS1656, D7S3048, D10S1248 and D19S253) in Beijing Han population. METHODS The DNA of 300 unrelated individuals in Beijing Han population were PCR amplified using GoldeneyeM DNA identification system 18NC kit, and the PCR products were analyzed by electrophoresis through 3130XL genetic analyzer. The fragment sizes of alleles were taken subsequently by GeneMapper v3.2. RESULTS The distributions of genotype frequencies of 16 non-CODIS STR loci in Beijing Han population satisfied the Hardy-Weinberg equilibration. The population genetic parameters were obtained as followings: heterozygosity was 0.677-0.873; discrimination power, 0.890-0.967; probability of paternity exclusion, 0.393-0.741; and polymorphism information content, 0.706-0.853. CONCLUSION These 16 non-CODIS STR loci show great genetic polymorphisms in Beijing Han population, and are useful for the research of population genetics and forensic application.

Improvements in and relating to methods and apparatus for comparison

Abstract: Apparatus and a computer implemented method are provided for comparing a first representation of an identifier with a second representation of an identifier to establish a likelihood ratio considering the probability the first representation and second representation originate from the same identifier and the probability of the first representation and second representation originate from different identifiers. The approach generates one or more variant expressions from the first representation, second representation and other representations. A boundary around the expression of the second representation is used to establish the number of the variant expressions of the expression of the first representation within it and the number of variant expressions of the expressions of the other representations within it and so provide the measure of comparison between the first representation of the identifier and the second representation of the identifier from the first value and second value.

Genetic polymorphisms of 10 short tandem repeat loci in Lhasa Tibetans in China

Abstract: Objective To investigate genetic polymorphisms of 10 short tandem repeats loci （D6S1043, D7S3048, D9S925, D10S2325, Dl1S2368, D14S608, D15S659, D17S1290, D20S470 and GATA198B05） in Tibetans from Lhasa, China. Methods Following extraction, DNA from 208 unrelated Tibetan individuals was amplified with a self-designed multiplex PCR system. The amplified fragments were separated by electrophoresis on an ABI 3130 Genetic Analyzer and analyzed with GeneMapper 3. 2 software. Results The distributions of genotype for the 10 STR loci in the population were in accordance with Hardy-Weinberg equilibrium. The polymorphism information component for the 10 loci was 0. 750- 0. 860, the degree of heterozygosity was 0. 726-0. 865, the discrimination power was 0.9]9-0. 968, and the probability of exclusion was O. 470-0. 725. The combined probability of exclusion and combined discrimination power was 0. 9998 and 0. 999999999997, respectively. Conclusion Above STR loci have high probability of exclusion and discrimination power, which can be used as candidate markers for population genetic research and forensic practice for Tibetans from Lhasa, China. Key words: Forensic biological evidence Short tandem repeats Genetic polymorphism; Tibetan

Ink Comparison and Interpretation

Abstract: This article focuses on the interpretation of the outcome of forensic ink analysis. Forensic analysis of ink can provide valuable information to forensic investigation of questioned documents. Ink analysis can be interpreted in a comparison process or in an identification process. This article formalizes these two processes and discusses the current limitations of the interpretation of ink analysis. Keywords: ink; source; comparison; identification; interpretation

pH Gradient Manipulation within the pH 4–6 Range Using Separator Isoelectric Focusing

Abstract: A number of different amphoteric buffer constituents (separators) have been evaluated with regard to their effectiveness in creating narrow pH gradients within a range of pH 4-6 in isoelectric focusing gels. Judicious mixing of carrier ampholytes and separators allowed much flexibility in choosing narrow pH gradients less than 1 pH unit wide within the pH 4-6 range. Provided gels were run under the same conditions and for the same period of time, pH gradients were reproducible under the conditions described. Finally, the uses of thickness-modified gradients and the separator N-(2-hydroxyethyl)piperazine-N′2-sulphonic acid (HEPES) have been examined for flattening the gradient produced by pH 4.5-5.4 Pharmalyte carrier ampholytes.