International Science and Technology Center

About: International Science and Technology Center is a nonprofit organization based out in Moscow, Russia. It is known for research contribution in the topics: Genome & Whole genome sequencing. The organization has 26 authors who have published 25 publications receiving 281 citations. The organization is also known as: ISTC.

The prebiotic potential of polysaccharides and extracts of seaweeds

Abstract: Based on our own and current literature data, we analyzed the prebiotic properties of polysaccharides and extracts of seaweeds. The role of prebiotics, particularly polysaccharides, in the normalization of intestinal microflora is discussed; consideration is also given to the possibility of seaweed polysaccharide fermentation by colonic microflora and selective stimulation of the growth of colonic bifidobacteria. This review also analyzes other useful properties of seaweed polysaccharides and discusses the prospects for inclusion of seaweed polysaccharides in the composition of functional nutrition products for the purposes of correcting intestinal bacterial disorders and inflammatory processes and normalizing the immune and metabolic status.

Probiotic Properties of Lactobacillus crispatus 2,029: Homeostatic Interaction with Cervicovaginal Epithelial Cells and Antagonistic Activity to Genitourinary Pathogens

Abstract: Lactobacillus crispatus 2029 isolated upon investigation of vaginal lactobacilli of healthy women of reproductive age was selected as a probiotic candidate. The aim of the present study was elucidation of the role of L. crispatus 2029 in resistance of the female reproductive tract to genitourinary pathogens using cervicovaginal epithelial model. Lactobacillus crispatus 2029 has surface layers (S-layers), which completely surround cells as the outermost component of their envelope. S-layers are responsible for the adhesion of lactobacilli on the surface of cervicovaginal epithelial cells. Study of interactions between L. crispatus 2029 and a type IV collagen, a major molecular component of epithelial cell extracellular matrix, showed that 125I-labeled type IV collagen binds to lactobacilli with high affinity (Kd = (8.0 ± 0.7) × 10(-10) M). Lactobacillus crispatus 2029 consistently colonized epithelial cells. There were no toxicity, epithelial damage and apoptosis after 24 h of colonization. Electronic microscope images demonstrated intimate association between L. crispatus 2029 and epithelial cells. Upon binding to epithelial cells, lactobacilli were recognized by toll-like 2/6 receptors. Lactobacillus crispatus induced NF-κB activation in epithelial cells and did not induce expression of innate immunity mediators IL-8, IL-1β, IL-1α and TNF-α. Lactobacillus crispatus 2029 inhibited IL-8 production in epithelial cells induced by MALP-2 and increased production of anti-inflammatory cytokine IL-6, maintaining the homeostasis of female reproductive tract. Lactobacillus crispatus 2029 produced H2O2 and provided wide spectrum of antagonistic activity increasing colonization resistance to urinary tract infections by bacterial vaginosis and vulvovaginal candidiasis associated agents.

Use of Corynebacterium pseudodiphtheriticum for elimination of Staphylococcus aureus from the nasal cavity in volunteers exposed to abnormal microclimate and altered gaseous environment

Abstract: Effectiveness of saline suspension of Corynebacterium pseudodiphtheriticum containing spray was assessed in a 30-days chamber experiment modeling the effects of hyperthermia and polluted air on humans. Spray was targeted at eliminating Staphylococcus aureus from the nasal cavity of human subjects. Three of four volunteers became S. aureus carriers in the course of the experiment, and one was a chronic carrier of methicillin-resistant S. aureus (MRSA). Spray application eradicated S. aureus in three subjects and reduced its presence in the MRSA carrier. Results of the study suggest that C. pseudodiphtheriticum can be used for control of S. aureus in the nasal environment. However, further investigations are required.

Poultry-beneficial solid-state Bacillus amyloliquefaciens B-1895 fermented soybean formulation.

Abstract: Birds were given a new formulation of the Bacillus amyloliquefaciens B-1895 solid-state fermented soybean that retained the spores of the aforementioned organism. Mass dynamics, feed flow rate and broiler performance were observed to evaluate the efficacy of the formulation. At each time point, the live mass was greater than that of the control group, reaching a difference of 7–8% by day 28. A difference of 5.3–8.8% was observed in feed conversion per kilogram live mass (1.97 kg in the controls as compared with 1.81–1.87 kg in experimental groups). This indicates a positive effect of the B. amyloliquefaciens B-1895 formulation on the live mass of broilers as well as on feed consumption.