Korea Forest Service

About: Korea Forest Service is a government organization based out in Daejeon, South Korea. It is known for research contribution in the topics: Environmental science & Ecosystem. The organization has 52 authors who have published 56 publications receiving 1122 citations.

Genetic variation in populations of Populus davidiana Dode based on microsatellite marker analysis

Abstract: Populus davidiana Dode, the only aspen in Korea, is useful for restoration because of its fast growth and vigorous reproduction. This study was conducted to estimate the genetic diversity and subdivision in populations of P. davidiana in South Korea using microsatellite markers. DNA from 113 individuals from five populations was amplified using five microsatellite primers. Fifty-three alleles were detected, and the expected heterozygosity was 0.603. The populations of P. davidiana in Korea have high genetic variation despite their peripheral distribution. RST (0.213) indicated a significant level of genetic subdivision among populations compared to the differentiation among other aspen populations. The high conservation value of the populations is attributed to its ecological and commercial importance.

Effects of altering medium strength and sucrose concentration on in vitro germination and seedling growth of Cypripedium macranthos Sw

Abstract: The genus Cypripedium, known as lady's slipper orchid, has been considered to have a considerable marketability as a potted and garden plant with beautiful flowers; however, this species is becoming endangered and, in some places extinct, due to habitat destruction and illegal collections. As such, an optimized artificial propagation system is necessary for its conservation and horticultural cultivation. For the establishment of the in vitro proliferation of the endangered Cypripedium macranthos Sw., native to Korea, the effects of medium strength and sucrose concentrations on germination, protocorm formation and seedling growth were investigated through asymbiotic seed culture. The highest germination rate and protocorm formation rate were obtained with a 1/4 MS medium; higher MS medium strengths did not generate the favorable conditions required for germination and protocorm growth. The overall growth characteristics of roots and buds developed from protocorms were best in cultures of 1/4 MS medium. On this medium containing 10 g·L -1 sucrose, both the germination rate and protocorm formation rate increased significantly. The general growth properties of seedlings developed on the medium with 10 g·L -1 sucrose were best, showing the highest bud formation rate and root number. Our results demonstrate that the 1/4 MS medium, supplemented with 10 g·L -1 sucrose, could improve in vitro germination, and facilitate the growth of seedlings developed from the protocorms of C. macranthos Sw.

Improvement of asymbiotic seed germination and seedling development of Cypripedium macranthos Sw. with organic additives

Abstract: To find the optimal propagation condition for endangered Cypripedium macranthos Sw., also known as lady's slipper orchid, the effect of various organic additives on in vitro germination, protocorm formation and seedling growth was investigated during asymbiotic seed culture. When 100 ml·L -1 coconut water was added to the basal medium, the highest germination rate and protocorm formation rate were achieved, with 70.8% and 74.2% respectively. Supplementation of phloem sap from birch tree or maple tree also showed a facilitating effect to improve the germination and protocorm development. With 100 ml·L -1 birch sap or maple sap, both the germination and protocorm formation rates were roughly more than 65% and 68%. The roots and buds of the seedlings grew vigorously in the medium containing 100 ml·L -1 coconut water or phloem sap, in particular, their bud formation rates increased by more than 70%. Addition of banana powder and peptone could not create a more significantly favorable culture condition, and non-addition had the worst results. Our results demonstrated that proper organic amendments such as coconut water and phloem sap might be preferred to in vitro germination and the growth of seedlings developed from the protocorm of C. macranthos Sw. during asymbiotic seed culture.