LGC

About: LGC is a based out in . It is known for research contribution in the topics: Glass ionomer cement & Dental cement. The organization has 661 authors who have published 710 publications receiving 17724 citations.

Harmonized guidelines for single-laboratory validation of methods of analysis (IUPAC Technical Report)

Abstract: Method validation is one of the measures universally recognized as a necessary part of a comprehensive system of quality assurance in analytical chemistry. In the past, ISO, IUPAC, and AOAC International have cooperated to produce agreed protocols or guidelines on the "Design, conduct and interpretation of method performance studies" [1], on the "Proficiency testing of (chemical) analytical laboratories" [2], on ''Internal quality control in analytical chemistry laboratories" [3], and on "The use of recovery information in analytical measurement" [4]. The Working Group that produced these protocols/guidelines has now been mandated by IUPAC to prepare guidelines on the single-laboratory validation of methods of analysis. These guidelines provide minimum recommendations on procedures that should be employed to ensure adequate validation of analytical methods. A draft of the guidelines has been discussed at an International Symposium on the Harmonization of Quality Assurance Systems in Chemical Laboratory, the proceedings from which have been published by the UK Royal Society of Chemistry.

The glass‐ionomer cement, a new translucent dental filling material

Abstract: A new translucent cement, the product of the reaction between ion-leachable glass and an aqueous solution of polyacrylic acid, is described. Its properties, with particular reference to dental applications, are reported and a proposed setting mechanism is advanced.

Improved adhesion of a glass ionomer cement to dentin and enamel.

Abstract: A study has been made on improving the adhesion of a glass ionomer cement by the chemical treatment of dentin and enamel surfaces. The most effective surface conditioners were high molecular weight substances containing a multiplicity of functional groups capable of hydrogen bonding. Low molecular weight chelating agents which dissolve calciferous material and dramatically alter the surfaces of enamel and dentin are less effective.

Mechanism of Adhesion of Polyelectrolyte Cements to Hydroxyapatite

Abstract: The interaction between polyacrylate ions and hydroxyapatite was examined using extraction techniques and infra-red spectroscopy. These studies have shown that polyacrylate ions become irreversibly attached to the surface of hydroxyapatite by displacing existing phosphate ions. The mechanism is not one of simple ion exchange, since calcium ions are displaced by the phosphate ions.

Polymerase chain reaction (PCR) detection of Listeria monocytogenes in diluted milk and reversal of PCR inhibition caused by calcium ions.

Abstract: DNA from Listeria monocytogenes was used as the model system from this investigation, with PCR primers based on the listeriolysin O gene. Under standard polymerase chain reaction (PCR) conditions and with no prior treatment, amplification failed in the presence of more than 5% milk. Since inhibition of the PCR occurred at the same milk concentrations with full fat, half fat and fat-free milk, inhibition was not attributed to the fat content of the milk. Calcium ions were, however, identified as a major source of PCR inhibition. The results demonstrated that the inhibitory effects of calcium ions and milk could be partially reversed by increasing the magnesium concentration in the reaction to well above the standard levels normally required for PCR. This work has important implications for the use of the PCR in the direct detection of food pathogens.