Showing papers in "Analytical Biochemistry in 1964"

TL;DR: A unit for disaccharidase activity is defined that is in accord with recommendations made by the Joint Sub-Commission on Clinical Enzyme Units of the International Unions of Biochemistry and of Pure and Applied Chemistry.

TL;DR: Measurements on several dipeptides showed that proline peptide do not form ultraviolet-absorbing complexes with copper whereas prolyl peptides do form such complexes, which explains the finding that gelatin (which has a high proportion of proline and of hydroxyproline residues) is less reactive with copper than are the other proteins studied here.

TL;DR: An apparatus suitable for preparative temperature-regulated polyacrylamide gel electrophoresis in discontinuous buffer systems is described and preliminary application to enzymes has demonstrated the feasibility of purification and recovery of activity.

TL;DR: This method for the determination of phosphorus in amounts from 1.0 to 100 μg has been developed by reinvestigating and combining the desirable features of several previous methods and is fast, simple, and accurate.

TL;DR: It takes minutes to inactivate phosphatase completely, and allows measurable enzyme action to occur in plant tissue slices and cell suspensions, and in Chlorella extracts, the amount of ADP and UDP can be doubled by post mortem phosphat enzyme action.

TL;DR: The scintillation solvent system described (dioxane-water-naphthalene) deactivates silica, thereby preventing adsorption on particles of silica and self-absorption loss of many polar radioactive compounds.

TL;DR: A fluorometric method has been described that allows the microdetermination of glycolytic intermediates in extracts from tissues or celis and the applicability of the method to the quantitative assay of intermediates down to a fraction of a micromolar range of concentration in the assay system.

TL;DR: Two liquid scintillation counting techniques are described for the simultaneous assay of two BETA -emitting isotopes based on the empirical determination of the energy distribution for each isotope in various combinations of windows and the effects of known amounts of quenching on these distributions.

TL;DR: The fluorescence of FMA is quenched by combination with a thiol group and by reaction with the disulfide group at high pH so that the FMA method is at least fifty times more sensitive than the ninhydrin assay.

TL;DR: The method gives results identical to those obtained with a manual procedure, and enables one to carry out 100 or more triglyceride determinations per day.

TL;DR: A colorimetric assay for the quantitative determination of catecholic compounds was developed based on the observation that a red color was formed when nitrite was added to a solution containing pyrocatechol and sodium tungstate.

TL;DR: In this paper, the presence of C 14 -labeled impurities in commercially available sodium pyruvate-C 14 and the instability of the resulting solutions was investigated and demonstrated.

TL;DR: Cyanate accumulation was prevented by storage of neutral solutions of urea (prepared at temperatures not exceeding 25°) at 5°, or by buffering the urea solutions at pH 4.7.

TL;DR: A method for colorimetric differentiation and quantitation of glucosamine and galactosamine by substituting borate for the carbonate buffers in both the Morgan-Elson reaction and Elson-Morgan reaction has been presented.

TL;DR: Techniques for the gas-liquid chromatographic separation of hydroxy fatty acids and the rapid preparation, characterization, and quantitation of saturated and unsaturated 2-acetoxy fatty acid methyl esters ranging from 14–26 carbons encountered in sphingolipids are described.

TL;DR: The concentrations of Na +, K + , and Cl − determined by this method were compared with those obtained for Na + and K + by dry ashing and with that found for Cl − by the oxygen flask method: there was no significant difference between nitric acid extraction and the other methods.

TL;DR: Gel filtration has been used for the separation of the acidic oligosaccharides obtained by testicular hyaluronidase treatment of hyaluronic acid and of chondroitin 4-sulfate by anasalysis before and after reduction with sodium borohydride.

TL;DR: Increasing the concentration of acrylamide gel decreases the absolute electrophoretic mobility of hemoglobins, egg albumins, and serum albumin polymers.

TL;DR: Multibore columns were constructed and found to have a high capacity, give sharp resolution, with less adsorbent and smaller amounts of eluting solvent, which has proved to be of great benefit in routine analysis.

TL;DR: Crystalline phosphorylase b and the amylo-1,6-glucosidase preparation can be used for the structural analysis of glycogen samples and for a specific enzymic microdetermination of glycogens in intestinal smooth muscle (taenia coli of the guinea pig), a tissue containing low concentrations of this polysaccharide.

TL;DR: Sugs can be analyzed quantitatively as their trimethylsilyl derivatives using gas-liquid chromatography, and this use of GLC will make it easier for the biochemist to attack problems in carbohydrate chemistry by enabling quantitative work to be done readily on mixtures heretofore difficult to analyze.

TL;DR: A combination unit consisting of a Gilford model 2000 multiple sample absorbance recorder, a Beckman DU monochromator with thermostated chamber, and a Technicon large sampler and proportioning pump is described.

TL;DR: A complete alignment procedure, utilizing new adjustable optical elecments, is presented for the schlieren/interference optical system of the analytical ultracentrifuge and a “troubleshooting” chart for evaluating and eliminating anomalies appearing inSchlieren and interference patterns is presented.

TL;DR: By utilizing DNA and T3 bacteriophage as test materials, good isopyenic banding in CsCl was obtained in #30 and #40 Spinco fixed-angle rotors.