Showing papers in "Archives of Andrology in 1999"

Chronic cadmium toxicity to sperm of heavy cigarette smokers: immunomodulation by zinc

Abstract: The aim of the study was to investigate the role of zinc therapy in 125 male cigarette smokers with infertility. The mechanism involved in the zinc/cadmium relationship was evaluated through the effect of a zinc-deficient diet and supplementation on testes of male adult Sprague-Drew rats. Heavy smoking was associated with low sperm count, motility, and morphology and increased seminal cadmium levels. Zinc therapy improved sperm quality and increased seminal IL-4, but reduced TNF-alpha and IFN-gamma. A zinc-deficient diet led to high cadmium testicular accumulation comparable with those supplemented with cadmium. Cadmium had a linear correlation with TNF-alpha and IFN-gamma, but not with IL-4. Cytology of testicular aspirate and histopathology were normal in supplemented groups as in controls. These results indicate that zinc modulates the putative effect of cadmium through its enhancement of T-helper 2 cytokines expression and down-regulation of T-helper 1 cytokines.

Influence of chronic morphine exposure on serum LH, FSH, testosterone levels, and body and testicular weights in the developing male rat.

Abstract: Opiate abuse has been a matter of serious concern in male adolescents. This study investigates the effects of chronic morphine administration on serum luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone levels, testicular histology, and body and testes weight in developing male rats. Animals were subcutaneously injected with morphine (5 mg/kg) or saline (1 mL/kg) twice daily for 30 days. Body weight determinations and injections were carried out under light ether anesthesia. At the end of the experiments, the rats were decapitated and blood samples were collected. Serum levels of LH and FSH were measured. Chronic morphine administration significantly decreased decreased serum testosterone (p < .02) and LH (p < .01) levels, but not FSH release compared to controls. Morphine exposure reduced body weight (p < .01), but had no significant effect on the testicular weight. When the testicular tissue was histologically examined, structural features of the seminiferous tubules and Leydig cells were similar in both saline and morphine-treated animals. The results suggest that opiates affect testosterone release through the hypothalamo-hypophyseal-gonadal axis rather than by a local testicular mechanism. Chronic morphine exposure during sexual maturation may have long-term endocrine disturbances in male rats.

Expression and phosphorylation of mitogen-activated protein kinases during spermatogenesis and epididymal sperm maturation in mice

Abstract: The expression and phosphorylation/dephosphorylation of mitogen-activated protein (MAP) kinases during mouse spermatogenesis and epididymal sperm maturation have been investigated by immunoblotting and immunohistochemical staining with commercially available anti-ERK2 and anti-Active MAPK antibodies. Two forms of MAP kinases, p42ERK2 and p44ERK1, were expressed in a similar amount in spermatogenic cells at different stages. ERK1 and ERK2 were phosphorylated (activated) in early spermatogenic cells from primitive spermatogonia to zygotene primary spermatocytes, while only a small quantity of phosphorylated MAP kinases could be detected in pachytene primary spermatocytes and spermatids. MAP kinase activity in primative spermatogonia and preleptotene primary spermatocytes was the highest among spermatogenic cells. ERK1 and ERK2 were also present in epididymal spermatozoa, and their phosphorylation was increased while spermatozoa pass through epididymis and vas deferens for maturation. It would appear that MAP kinase activation may contribute to the mitotic proliferation of primative spermatogonia, an early phase of spermatogenic meiosis, and, later, sperm motility acquirement.

Sexual satisfaction in male infertility

Abstract: Infertility is proposed to be a continuing stressor for couples suffering from involuntary childlessness. A long duration of the desire for a child and, correspondingly, a longer period of diagnostic and treatment procedures could have a negative impact on sexual satisfaction, thus leading to an unfavorable psychological circuit. The present evaluation should clarify the state of sexual satisfaction and relationships, with relevant parameters in 68 men with fertility problems, of couples with involuntary childlessness. Subjects reported relatively high average levels of present sexual satisfaction with only nonsignificant lower scores (p=. 08) compared to recalled sexual satisfaction prior to diagnosis of infertility. Multiple regression analyses revealed that a positive age difference between men and their spouses (p=. 042) and a higher weekly coitus frequency (p=. 002) were the only significant parameters associated independently with higher sexual satisfaction. Neither the age of partners, attitudes to...

Male fertility of kidney transplant patients with one to ten years of evolution using a conventional immunosuppressive regimen

Abstract: The reproductive functions and hormone serum levels of 55 male kidney transplant recipients were assessed. Patients underwent peritoneal dialysis before transplantation and were given immunosuppressive therapy afterward for 1 to 10 years. Spermatobioscopies were performed, and serum urea, creatinine, luteinizing hormone (LH), follicle-stimulating hormone (FSH), prolactin (PRL), and testosterone (T) levels were determined. Average serum urea and creatinine levels were 54.6+/-1.4 and 3+/-1.3 mg/dL, respectively. The average serum hormone levels were 3.2+/-2 mIU/mL (LH), 6.3+/-1.7 mIU/mL (FSH), 11.7+/-1.5 ng/mL (PRL), and 23+/-1.4 pg/mL (T). Libido reduction was reported in 88% of patients within 8 months following transplantation. Normozoospermia was seen in 47.3% of the patients, asthenozoospermia in 18.2% oligozoospermia in 14.5%, while oligoteratozoospermia, asthenoteratozoospermia, oligoasthenozoospermia, oligoasthenoteratozoospermia, and azoospermia were seen in the rest. Twenty-six patients procreated one or more children after transplantation; 36.6% of those children were premature but nonetheless healthy. No association existed between the post-transplant period and urea or creatinine levels. Significant differences were found when LH levels and sperm motility were assessed. Also, statistically significant differences were found when duration of dialysis, FSH levels, sperm counts, morphology, and motility between posttransplant fertile and infertile patients were correlated. In conclusion, there was an adequate recovery of sexual and reproductive functions in most patients subjected to kidney transplantation and conventional immunosuppressants.

Degelification of alpaca semen and the effect of dilution rates on artificial insemination outcome.

Abstract: Degelification of highly viscous alpaca semen was attempted using two enzymes: trypsin and collagenase. Dilution effect on artificial insemination was determined in alpacas. Semen from 4 male alpacas was collected, degelified, diluted, and inseminated into 80 female alpacas. Degelification was achieved adding trypsin and collagenase enzymes to fresh semen samples. Semen was diluted with egg-yolk glucose citrate to give concentrations of 4, 8, and 12 million spermatozoa/mL. Females were induced to ovulate with human chorionic gonadotropin and then inseminated deep into the uterine horns. Analysis of variance was used to determine differences in the effect of trypsin and collagenase on sperm acrosome and on motility and live spermatozoa. The chi-square test was used to determine differences in pregnancy of artificially inseminated females. Semen was degelified with different concentrations of trypsin and collagenase. There were differences (p <. 05) in the pregnancy rate of female alpacas inseminated wit...

Identification of insulin-like growth factor (igf)-1 receptor in human sperm cell

Abstract: The present study was conducted to examine the presence of insulin-like growth factor- receptor (IGF-1R) in human sperm cell. The IGF-1R monoclonal antibody (MAb) specifically immunoprecipitated two protein bands of approximately 135 and approximately 220 kD, respectively, from the reduced human sperm extract. These bands correspond to the alpha-subunit and partially reduced IGF-1R molecule, respectively. The immunocytochemistry experiments indicated the presence of IGF-1R primarily in the equatorial regions, along with a weak binding in the acrosomal regions of human spermatozoa. These data indicate that the IGF-1 receptor is present in the human sperm cell. Since the IGF-1 receptor has tyrosine kinase activity and its ligand IGF-1 is present in the seminal plasma, the IGF-1 system (IGF-1R/IGF-1/IGF-binding protein) may be involved in the signal transduction pathway leading to sperm capacitation and acrosome exocytosis.

Expression of heat shock proteins in developing and degenerating rat testes.

Abstract: In the testis, several types of heat shock proteins (HSPs) have been identified and characterized, although the cellular basis of the HSPs remains elusive. In the present study, alterations in the cellular localization of HSPs, including HSP 25, 60, 70, and 90, were studied during the developing and degenerating periods in the rat testis using immunohistochemistry and Western blotting. HSP25 was expressed in neither germ cells nor somatic cells on all days examined. In contrast, HSP 60 was expressed in Leydig cells during neonatal and prepuberty periods, and only in spermatogonia and primary spermatocytes after puberty. HSPs 70 and 90 were expressed in germ cells, Sertoli cells, and Leydig cells during neonatal and early developing testes, and in spermatocytes and round spermatids after puberty. Besides, there was faint expression of HSP 90 protein in spermatogonia in this period. In the degenerative condition, all HSP proteins were markedly expressed in germ cells after surgery. It would appear that HSPs play roles in unique homeostasis in testes.

Perceptions of and risk factors for andropause.

Abstract: There has been some controversy about whether males undergo andropause (male equivalent of a menopause). This study seeks to describe how older males perceive and understand this entity. It also explores the possibility of an association between the age of onset of andropause and risk factors such as ethnic origin, smoking, alcohol, hypertension, and cerebrovascular disease. A nonexperimental, cross-sectional study was conducted at the outpatient clinic at a Veterans Affairs Medical Center. Subjects were interviewed by a single investigator and answered a questionnaire consisting of 22 items, including information on patient demographics, patient understanding of andropause, patient understanding of possible interventions with hormonal therapy, medical and drug history, and social habits such as smoking and drinking. Altogether 302 male patients were recruited: 71% of the survey population were above the age of 60 years, and 87% were white, 6% Hispanic, and 5% black. Patient knowledge of andropause was lacking, though the majority (70%) expressed an interest in getting further knowledge. The most frequent age for onset of symptoms related to andropause was 51-60 years, and patients reported symptoms such as impotence, weakness, and memory loss. The next most common age associated with onset was 61-70 years. Multivariate regression analysis revealed that smoking more than 10 cigarettes a day was independently associated with an earlier onset of andropause symptoms bringing down the age of onset to below 50 years (p = .01, OR = 2.5, CI = 1.2-5.3). We found no association with risk factors such as ethnicity or alcohol. Andropause is experienced by many older males but knowledge of the entity is nonuniform. Smokers are at risk for an earlier onset of andropause. This is the first study to look at risk factors for andropause and the results are consistent with studies in females on smoking and menopause.

Sex chromosome aneuploidies in sperm of 47,XYY men.

Abstract: The sex chromosomal equipment in 26,675 sperm of 47,XYY males was analyzed. A total of 5.78% of the nuclei exhibited sex chromosome hyperhaploidy. Six studies have analyzed the sperm of 10 XYY patients and, although these studies indicated some degree of elimination of the extra Y chromosome during spermatogenesis, a certain percentage of XYY germinal cells may also be able to achieve meiosis and produce sperm with gonosomal disomies. All these studies show an increased incidence of gonosomal aneuploidies in sperm, but there are significant discrepancies concerning the extent of these abnormalities. The global frequencies of sperm with an abnormal number of sex chromosomes ranged from 0.578 to 13.91%, depending on the patients. There are several explanations for these discrepancies: differences attributed to fluorescence in situ hybridization methodology, the use of dual or multicolor FISH, recruitment, interindividual variations, and intraindividual variations. This study reports an additional series obtained from another XYY individual and compares and discusses the data on gonosomal hyperhaploidies in sperm of 47 XYY males using in situ hybridization analyses.

Physioanatomic entirety of external anal sphincter with bulbocavernosus muscle.

Abstract: Stimulation of the glans penis evokes contraction of both the bulbocavernosus muscles (BCM) and the external anal sphincter (EAS). This synchronous contraction of the two muscles led us to study their physioanatomic relationship and possible role in erection and ejaculation. Fifteen male cadavers were studied (8 neonatal deaths and 7 adults; mean age 48 years) by dissection. The bulbocavernosus reflex action was performed in 12 healthy male volunteers (mean age 37 years) before and after anesthetizing the EAS. The response of the EAS and BCM to inferior rectal nerve stimulation was assessed in 6 men (mean age 41 years). The superficial fibers of the base loop of the EAS extended forward to the penile bulb where they were arranged into 3 groups: 1 median and 2 lateral. The median fibers, or the ''retractor penis muscle," were found inserted into the corpora cavernosa and the lateral fibers, or the ''compressor bulbae muscle," into the perineal membrane. Upon glans penis stimulation, both the EAS and BCM co...

Semen culture, leukocytospermia, and the presence of sperm antibodies in seminal hyperviscosity

Abstract: Seminal hyperviscosity is generally thought to reveal genitourinary infection. The aim of the present work was to study this hypothesis. A total of 65 semen samples were obtained from males presenting for infertility screening. The samples were evaluated according to WHO criteria and microbiologically investigated, including culturing for Mycoplasma hominis and Ureaplasma urealyticum, and microscopic observation of Chlamydia trachomatis by a direct fluorescence assay. Determination of local antisperm antibodies was performed. Semen was categorized according to consistency: normal (n = 31) and high (n = 34). No difference was recorded either in the number of positive cultures, or in the number of species found in each sample. The number of white blood cells and the percentage of antibody-bound sperm showed no difference in the groups under study. There was no association between seminal hyperviscosity, positivity in semen cultures, number of species isolated in semen cultures, leukospermia, or presence of sperm antibodies. Further studies should be performed considering the evolution of the genital-infected patients to clarify the etiology of the hyperviscosity.

Male fertility in long-term survivors of childhood ALL.

Abstract: A study of fertility was conducted in postpubertal male patients who had been treated for acute lymphoblastic leukemia (ALL) during childhood or adolescence between 1970 and 1980. Thirteen men (age 18 to 35 years) participated on a volunteer basis. Their age at diagnosis was between 2 and 15 years. Therapy followed the protocol ''Memphis VII (Pinkel)." Interview, physical examination, andrological studies (ejaculate), and hormone status (luteinizing hormone, follicle-stimulating hormone, and testosterone) were performed at least 5 years after completion of therapy. No normozoospermia was achieved; 10 patients were identified with asthenozoospermia and 3 patients with azoospermia. With respect to these data, patients treated for ALL between 1970 and 1980 have more significantly impaired spermatogenesis than expected.

Semen analysis in men from Mérida, Venezuela, over a 15-year period.

Abstract: To determine the possible changes in semen quality of Venezuelan men from 1981 to 1995, a retrospective analysis of semen volume and sperm concentrations was carried out for 2313 men from infertile couples. According to the sperm counts the sample was categorized in four groups: A, 0 sperm; B, <20 x 106 sperm/mL; C, 20-200 x 106 sperm/mL; D, 200 x 106 sperm/mL. The percentage of men in each group was 9.1, 18.8, 63.1, and 9.0%, respectively. The frequency of azoospermia and oligozoospermia (groups A and B) did not change over the last 15 years. On the contrary, the frequency showed a significant increase in group C and a decrease in group D. The range of the means of semen volume was 2.6-3.6 mL, linear regression analysis did not show a decrease in seminal fluid volume over time. The range of the means of sperm concentrations were 6.2-12.0 x 106 sperm/mL, 73 -100 x 106 sperm/mL), and 230-340 sperm x 106/mL in groups B, C, and D, respectively. Linear regression analysis revealed a significant reduction i...

Influence of progesterone and GABAA receptor on calcium mobilization during human sperm acrosome reaction.

Abstract: At fertilization, mammalian sperm has to undergo morphological changes of acrosome, namely acrosome reaction (AR), during which a dramatic increase of cytosolic calcium in consequence of extracellular calcium influx induces acrosomal exocytosis. It has been reported that progesterone is capable of inducing mammalian sperm AR. Several authors insisted that the agent, which was so far understood to bind with intracellular receptor, might act as an agonist against cell surface r-aminobutyric acid with type A (GABAA) receptor. The mode of action is, however, still in controversy. To investigate whether progesterone-induced AR is mediated by GABAA receptor, the present study examined pharmacologically the actions of progesterone on the morphological changes of acrosome and calcium mobilization during human sperm AR. Progesterone (15 microM) stimulated AR and increased cytosolic calcium, and the AR rate was further promoted by the coexistence of GABA (15 microM). Then these phenomena were suppressed by an antagonist of GABAA receptor (bicuculline, 10 microM), a blocker of GABAA receptor-coupled chloride channel (picrotoxin, 200 microM) and an antagonist of receptor-operated calcium channel (Lantan, 250 microM), respectively. These results indicated that the complex work of GABAA receptor-chloride channel and receptor operated calcium channel might participate in progesterone-induced AR and the transient increase of cytosolic calcium.

Effect of cyclosporine on human sperm motility in vitro

Abstract: Cyclosporine affects motility and viability of human sperm when incubated together in vitro. Sperm motility was almost reduced to nil following 10 min of incubation with cyclosporine at a concentration of 1 mg/mL. However, 200 mug/mL of the drug has no effect on motility and viability when tested for up to 60 min under standard laboratory conditions. Cyclosporine effect on sperm was both dose and time dependent. Sperm sensitivity and susceptibility to cyclosporine even to lower doses increased significantly following withdrawal of bovine serum albumin from the incubating medium. Compared to untreated controls, lactate dehydrogenase was estimated higher by more than 2 to 4 times in the spermfree incubating media, suggesting an altered membrane porosity in the affected spermatozoa.

Absence of effect of recombinant growth hormone to classic gonadotropin treatment on spermatogenesis of patients with severe hypogonadotropic hypogonadism

Abstract: Encouraging clinical results have suggested a complimentary or permissive role of growth hormone (GH) to gonadotropin in inducing spermatogenesis in patients with hypogonadotropic hypogonadism, who did not respond to gonadotropin therapy alone. This study evaluates the effects of GH on spermatogenesis in four azoospermic hypogonadic hypogonadotropic (HH) men, treated initially for 6 months with gonadotropins (GN) alone (phase I), followed by a 6 months cotreatment of GH with GN (phase II). Plasma testosterone (T), IGF-1 levels, testicular volume, and spermiogram were evaluated under basal conditions and every 3 months during treatment. GN treatment alone induced a significant increase of T levels and semen volume, whereas testicular volume increased very little; all patients remained azoospermic. The combined GN-GH treatment induced, as expected, an increase in IGF-1 levels and an increase of T levels to values in the normal male range as well as a further, moderate, increase in testicular volume; azoospe...

Quantitative ultramorphological analysis of human sperm: fifteen years of experience in the diagnosis and management of male factor infertility.

Abstract: The advantages of quantitative ultramorphological (QUM) sperm analysis in the diagnosis and treatment of male infertility are presented. The QUM methodology is based on three elements: complementary scanning electron microscopy and transmission electron microscopy observations of 7 sperm cell subcellular organelles (acrosome, postacrosomal lamina, nucleus, neck, axoneme, mitochondrial sheath, and outer dense fibers); systematic classification of the specific ultramorphological malformations into 4 pathological and the normal categories, indicating the morphological state of each subcellular organelle; and comparison between well-defined reference groups with opposite fertility status or treatment conditions. QUM has established 2 indices for the in vivo and in vitro male fertility potential: (1) Natural Fertility Index (NFI), with accurate prediction (97% sensitivity and 90% specificity) of 80% of the male patients; and (2) IVF score, with prediction of 76% of the nonfertilizing and 90% of fertilizing IVF groups. QUM has enabled assessment of ultramorphological indications for varicocele and radiation exposure. Varicocele causes defects in sperm head organelles related to early spermatid development, whereas ionizing radiation causes amorphous head shape. QUM established criteria for specific non-in-vitro therapeutic interventions, including varicocelectomy, follicle-stimulating hormone (FSH) administration, and acupuncture. The varicocele index enabled correct classification of 79 and 89% of patients with and without varicocele. Males with idiopathic impairment of sperm acrosome and nucleus are potential responders to FSH treatment, whereas patients exhibiting low sperm activity are candidates for acupuncture treatment. Patients with a low Natural Fertility Index are recommended for an assisted reproduction technique (ART), based on the ultramorphology of the tail axoneme. Patients who achieved pregnancy following intrauterine insemination or in vitro fertilization and those whose wives conceived only following intracytoplasmic sperm injection were classified with accuracy of 78 and 74%, respectively. QUM sperm analysis is clinically informative, nontraumatic, and cost-effective, and is recommended when the male infertility factor cannot be clearly diagnosed by routine tests prior to first ART trial.

Chlamydia trachomatis detected by ligase chain reaction in the semen of asymptomatic patients without pyospermia or pyuria.

Abstract: To determine whether an absence of leukocytes in semen and urine predicts an absence of Chlamydia trachomatis in asymptomatic infertile men, chlamydial DNA was detected in subjects' semen and urine by ligase chain reaction (LCR). Ninety-eight infertile men were studied, including 39 cases of oligozoospermia, 19 of azoospermia, 16 of asthenozoospermia, and 24 of normozoospermia. None of the subjects had pyospermia or pyuria. C. trachomatis was detected by LCR. Antichlamydial and antisperm antibody were also measured. C. trachomatis was detected by LCR in the semen of only 1 of 98 patients (1.02%), but not in the urine samples. In C. trachomatis-positive patients LCR, IgG, and IgA levels were higher than normal. No antisperm antibody was detected. Even if leukocytes are not observed in the semen and urine of asymptomatic infertile men, the presence of C. trachomatis in semen specimens is rarely observed. Therefore, it should be noted that the presence of C. trachomatis in such cases is addressed in the context of artificial insemination and other assisted reproductive technologies.

Molecular cloning and characterization of a novel testis-specific nucleoporin-related gene.

Abstract: A 20-kDa sperm membrane protein cDNA, designated as RSD-1, was isolated by epitope selection from a rat testis lambda gtll expression library. RSD-1 was used as a probe to screen a human testis lambda ZAPII cDNA expression library. A cDNA designated as BS-63 was isolated and found to consist of 1933 bp with an open reading frame of 1824 bp and assigned the accession number U64675 by GenBank. The deduced polypeptide consisted of 608 amino acid residues containing XFXFG or FG motifs that are characteristic of nuclear pore complex (NPC) proteins and act as potential binding sites for Ran. The N-terminal region has high homology with RanBP2/Nup358, a nucleoporin component, showing that BS-63 is a member of the NPC family. Northern blot analysis of mRNAs prepared from various human tissues shows that BS-63 is transcribed in two forms: 6.0 and 8.5 kb. The 8.5-kb transcript was present in low amounts in several somatic tissues, whereas the 6.0-kb transcript is expressed only in testis. In situ hybridization anal...

Prostate-specific antigen (PSA) in human seminal plasma.

Abstract: Prostate-specific antigen (PSA) was measured in seminal plasma and serum of 113 men under investigation for involuntary barrenness. PSA in seminal plasma was 0.4 +/- 0.3 (median 0.35) mg/mL and in serum 0.6 +/- 0.5 (median 0.6) ng/mL. The concentrations in these 2 compartments were independent of each other. The determination of PSA in neither blood serum nor seminal plasma brought new essentials for fertility status.

Quantitative ultramorphological (QUM) analysis of human sperm: diagnosis and management of male infertility.

Abstract: The advantages of quantitative ultramorphological (QUM) sperm analysis in the diagnosis and treatment of male infertility are presented. QUM methodology is based on three elements: (1) complementary SEM and TEM observations of 7 sperm cell subcellular organelles: acrosome, postacrosomal lamina, nucleus, neck, axoneme, mitochondrial sheath, and outer dense fibers; (2) systematic classification of the specific ultramorphological malformations into 4 pathological and the normal categories, which indicate the morphological state of each subcellular organelle; and (3) comparison between well-defined reference groups with opposite fertility status or treatment conditions. QUM analysis has enabled the establishment of two indices that optimally express the in vivo and in vitro male fertility potential: The Natural Fertility Index (NFI), which allowed an accurate prediction (97% sensitivity and 90% specificity) of 80% of the naturally fertile and suspected infertile male patients, and the in vitro fertilization (...

Influence of sperm plasma membrane destruction on human sperm head decondensation and pronuclear formation.

Abstract: This study investigated the ability of membrane-damaged sperm to undergo sperm head decondensation and male pronuclear formation following intracytoplasmic sperm injection. For induction of membrane-damage, sperm were exposed to 0.1% Triton X-100 for a few seconds. The single sperm curling (SSC) test was performed to evaluate the viability of the sperm. Of the injected oocytes 49 and 47% developed male pronuclei in the experimental and control groups, respectively. Sperm head decondensation was much higher in the membrane-damaged group (84 versus 70%). This study clearly shows that ICSI of membrane-damaged sperm does not affect pronuclear formation, although sperm head decondensation is much higher.

Sperm extracted and cryopreserved from testes several hours after death results in pregnancy following frozen embryo transfer: case report

Abstract: A 38-year-old male died suddenly on his honeymoon. Sperm was extracted from his testes 3 h following his death and cryopreserved. His wife had in vitro fertilization (IVF) and the eggs were inseminated by intracytoplasmic sperm injection (ICSI). None of the sperm were motile. Selection was based on softness and pliability. There were 4 embryos formed that cleaved, but only 2 were transferred on the retrieval cycle. The wife failed to conceive, but then had a second transfer of the 2 cryopreserved embryos. She achieved a chemical pregnancy with the beta-human chorionic gonadotropin level attaining a maximum level of 107 mIU/mL (rising from 19 mIU/mL). Though this retrieval cycle did not result in a successful pregnancy the achievement of a clinical pregnancy following frozen embryo transfer at least provides cautious optimism for other cases with similar conditions.

Influence of pentoxifylline on sperm membrane functional integrity

Abstract: In epididymal mouse spermatozoa, the effects of dibutyryl cyclic adenosine monophosphate 1 mmol/L (dbcAMP), pentoxifylline 5 mmol/L (PX), and/or mastoparan 50 mumol/L (MT) were evaluated for the following parameters: percentage of motile cells and response to hypoosmotic shock (HOS). The gametes were incubated during 80 min (A) or 200 min (B) in Tyrode's medium, and the drugs were added during the last 20 min. In A, dbcAMP + PX (61.5 +/- 5.4%; n = 10) enhanced and MT decreased significantly the population of motile cells (13.4 +/- 5.4%; n = 6) (control: 47.6 +/- 3.9%; n = 11). In B, PX significantly increased this parameter and MT plus PX also exerted a significant detrimental effect. Responses to HOS dropped significantly in the presence of PX + MT in A or in B; in this latter condition a similar decrease was evoked by MT alone. A positive correlation between percentages of swollen and motile spermatozoa was detected in A or in B in samples incubated with PX (r =. 58, n = 11 and r =. 76, n = 10; p <. ...

Bromocriptine for infertile males with mild hyperprolactinemia: hormonal and spermatogenic effects

Abstract: To clarify the influence of hyperprolactinemia on spermatogenesis and steroidogenesis in infertile male patients, the serum prolactin (PRL), luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone, and estradiol concentrations were and the effect of bromocriptine treatment on spermatogenesis was examined. A total of 1234 patients were evaluated and 147 men had hyperprolactinemia. Of these, only 30 had PRL concentrations more than twice the upper limit of normal and most of them had a little excess over the upper limit. For 10 of these 30, serum hormone concentrations were measured and semen was analyzed before and after bromocriptine administration. No relationship between the PRL and other hormone concentrations was found. No changes were noted in the LH, FSH, testosterone, or estradiol concentrations, or in the sperm density and motility after treatment. The mean PRL decreased from 26.5 +/- 4.5 to 1.4 +/- 1.8 ng/mL. In infertile men who are mildly hyperprolactinemic, bromocriptine administration does not improve semen analysis, although it does normalize the PRL.

Pituitary and testicular function in adolescents with varicocele.

Abstract: The LH and FSH responses post GnRH, and the sex steroid hormone responses post hCG stimulation were evaluated in a group of 41 adolescents (mean age 15.09 +/- 2.27) with unilateral varicocele to study the relation between the pituitary gonadotropin and sex steroid hormone responses to the patients' age and pubertal stages. Twenty-two normal boys (mean age 14.90 +/- 1.97) also were studied. In both groups a GnRH stimulation test was performed. Besides this, an hCG test was performed in 7 patients and 7 controls. No significative differences in the LH and FSH responses to the GnRH stimulus were observed in 14 patients at Tanners' pubertal stages 3 to 4 compared to the controls. Twelve (44.44%) patients at Tanners' pubertal stage 5 presented an excessive LH response at 30 and 60 min after GnRH stimulation compared to 12 age- and pubertal stage-matched controls (p <. 001 and p <. 05, respectively). They also presented an excessive FSH response at 30 min (p <. 05) post-GnRH stimulation. Correlation an...

Spontaneous occurrence of vasculitis-like lesions in male reproductive tissues in mice: a histological study

Abstract: Vasculitis involving testes, epididymides, and vasa deferentia is considered to be a rare disease but has often been reported in man. In the present study, reproductive tissues of young and aged mice were examined to determine whether spontaneous vasculitis occurs in them. Testes, ductuli efferentes, epididymides, and vasa deferentia were obtained from young and aged BALB/c mice together with some nonreproductive organs for histological observation at the light microscopic level. The examination revealed that vasculitis-like lesions comparable to those in man were significantly present in the epididymides and vasa deferentia of aged but not young mice. However, no significant lesions were found in the testes and ductuli efferentes. Furthermore, other organs, such as salivary glands, thyroid glands, livers, pancreases, and kidneys, were also negative for the lesions. These results indicate that the epididymis and vas deferens are spontaneously apt to be affected by vasculitis-like lesions with advancing age, but that the lesions are not due to a systemic vasculitis disease.

Androgen-dependent synthesis/secretion of caltrin, calcium transport inhibitor protein of mammalian seminal vesicle.

Abstract: Effects of androgen status on the synthesis and secretion of rat caltrin have been studied by three different procedures: a) immunocytochemistry in seminal vesicle tissues; b) polyacrylamide gel electrophoresis and Western immunostaining of seminal vesicle secretion; and c) evaluation of trypsin inhibitory activity of the seminal vesicle secretion. Rat caltrin has been immunolocalized in cells of the secretory epithelium, specifically in the electron-lucent halo of secretory granules which store and transport proteins to the lumen. No caltrin immunoreaction was detected 14 days postcastration, and the ultrastructure of the epithelial cells was markedly altered. SDS-PAGE and Western blotting of the seminal vesicle secretion revealed alterations in the protein pattern and loss of the caltrin-related immunoreactive bands. The 54-kDa caltrin-precursor protein and the 6.2-kDa active caltrin were absent. Trypsin inhibitory activity of the seminal secretion was reduced about 50% in castrated animals. Daily testosterone administration restored both the protein pattern and immunoreactivity of the seminal vesicle secretion, and, as expected, reversed the morphological alterations of the gland after 7 days of treatment. Trypsin--inhibitor effect of the secretion also returned to normal levels after fourteen days of testosterone administration. Data suggest that the synthesis and secretion of caltrin are testosterone-dependent processes.

Effect of experimentally induced renal failure on testicular testosterone synthesis in rats.

Abstract: Renal insufficiency is responsible for gonadal impairment, but the pathogenesis of testicular dysfunction remains unresolved. This study examines the possible role of the endocrine disturbance and angiotensin II-induced physiological abnormality for the pathogenesis of gonadal dysfunction of two different types of chronic renal failure. Chronic renal insufficiency was induced in rats given an adenine-excessive diet or in 5/6 nephrectomized animals. Circulating levels of blood urea nitrogen, creatinine, renin-angiotensin-aldosterone (R-A-A) system androstenedione, 17 alpha-hydroxy progesterone (17 alpha-OHP), testosterone, luteinizing hormone, and follicle-stimulating hormone were assayed. Systolic blood pressure, renal blood flow, and testicular blood flow were also determined. High serum levels of 17 alpha-OHP, androstenedione, and low testosterone were noted in the normotensive group. Enhanced R-A-A system decreased testicular blood flow and low testosterone were seen in the hypertensive group. The data provide evidence that gonadal dysfunction in adenine-induced renal failure appears to be caused by the suppression of 17 beta-hydroxysteroid oxydoreductase activity, and gonadal impairment in 5/6 nephrectomized uremia can be evoked by enhanced renin-angiotensin-aldosterone system and hypertension.