Showing papers in "Biochemical Medicine in 1974"

TL;DR: The synthesis and analytical use of succinyl-( l -alanine) 3 - p -nitroanilide, a new elastase substrate that is virtually not attacked by trypsin or chymotrypsin and does not undergo appreciable spontaneous hydrolysis is described.

TL;DR: A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented and can be used to accurately determine 0.1 μg of the vitamin in samples of plasma andOther biological tissues.

TL;DR: A simple and sensitive one-step chemical assay using dual-wavelength spectrophotometry for dopamine-β-hydroxylase (DBH) activity is described, permits the assay of the maximum velocity at a saturated concentration of substrate under the optimum conditions and is generally applicable to various tissues with low enzyme activity.

TL;DR: It is clear that a significant advance has been achieved by using a five-buffer lithium citrate system in combination with high buffer pressure and small sample volume, with well over 40 compounds measured to a high degree of accuracy.

TL;DR: The specificity of the enzyme glycine-N-acylase prepared from ox liver mitochondria for the coenzyme A derivatives of several organic acids has been investigated and closely parallel the degree of glycine conjugation found in patients with the organicacidaemias.

TL;DR: VMA, which hitherto has not been positively identified and quantified in CSF was easily determined by this method, and offers the advantages of determining, in a single sample, small amounts of these O-methylated metabolites of the catecholamines.

TL;DR: An improved spectrophotofluorometric method for the determination of methotrexate (MTX) in biological samples has been developed with special emphasis on the analysis of human plasma and obtained a more than 10-fold higher fluorescence using equal aliquots of plasma.

TL;DR: A method is described for the separate estimation of the glucuronide and sulphate conjugates of 3-methoxy-4-hydroxyphenylglycol (MOPEG) in urine and the possibility that the sulphatase or β-glucuronidase could have arisen centrally and the glucose peripherally is discussed.

TL;DR: The serum cyclic nucleotide phosphodiesterase activities of healthy persons and patients with several diseases were compared and a new assay was developed based on adsorption and elution of the product, nucleoside, from small cation exchange column.

TL;DR: The human liver pyruvate carboxylase has been shown to exhibit significant catalytic activity in the absence of the positive effector acetyl-CoA; and the catalytic properties of the acetylCoA-dependent and acetyl CoA-independent activities have been defined as discussed by the authors.

TL;DR: Human liver microsomes isolated from surgical biopsy specimen hydroxylate taurolithocholic acid in the 6α position requires NADPH and the identification of the reaction product as hyodeoxycholic acid is based on data obtained from gas chromatographic, thin-layer chromatography, and mass spectrometric analyses.

TL;DR: The Evelyn and Malloy procedure for methemoglobin determination in blood has been modified to decrease changes which occur in shed blood and in blood hemolysates.

TL;DR: The concentrations of taurocyamine, glycocyamine, γ-guanidinobutyric acid, and arginine in rodent brain tissue as well as human brain were determined and five unknown guanidino compounds were found.

TL;DR: There is a defect in the metabolism of both proline and hydroxyproline in this disorder, and that the prime o -aminobenzaldehyde positive compound in the urine of these patients is Δ 1 -pyrroline-3-hydroxy-5-carboxylic acid.

TL;DR: A simple, sensitive and precise method is described for the fluorometric determination of tocopherol in sheep plasma, with recoveries of 93–100% and variations with seasonal pasture change and pregnancy.

TL;DR: Intimamedia tissue from atherosclerotic rabbits showed a 15-fold increase in the amount of [U- 14 C]palmitic acid oxidized to CO 2 compared to normal intimamedia, suggesting that oxidation of free fatty acids may at least in part contribute to the increased oxygen uptake seen in atherosclerosis tissue.

TL;DR: Since Rhesus monkey LDL is similar to the human LDL, these genetically altered monkeys may serve as a useful experimental model in which to study human Type IIb hyperlipoproteinemia.

TL;DR: The ATP/serotonin ratio was significantly higher in D.S. platelets indicating that in relation to serotonin, there was adequate ATP available.

TL;DR: In this paper, a human skeletal muscle AMP-aminohydrolase from skeletal muscle has been purified four-hundredfold by chromatography on cellulose phosphate Enzyme was inactivated by dialysis against 1 m m EDTA and the activity could have been restored upon addition of some divalent cations.

TL;DR: A transient accumulation of endogenously produced orotic acid in the livers of rats treated with ammonium acetate was demonstrated and a twofold increase in the liver acid-soluble uracil nucleotide content was shown.

TL;DR: The results indicate that the human gastric mucosa contains at least three distinct classes of acid proteinase which are capable of attacking different peptide bonds.

TL;DR: The results indicate that the thyroid hormones mainly affect neutral steroid metabolism, and cholesterol absorption was increased in hypothyroid (PTU fed) rats and decreased in hyperthyroid (T4 treated) rats.

TL;DR: The adaptation to the auto-analyzer of a highly sensitive, quick and specific assay for quantitative determination of uronic acids in tissues and biological fluids is presented.

TL;DR: All of the ESF activity was recovered from a test volume of urine obtained from a patient with paroxysmal nocturnal hemoglobinuria, and test specimens of urine from anemic patients were concentrated to determine ESF levels of these patients.

TL;DR: A new fluorometric method for analysis ofcitric acid in blood and plasma was established, where the citric acid react with o -aminothiophenol in 35% phosphoric acid solution, at 125°C, for 15 hr.

TL;DR: It is suggested that, at the dose used in myasthenia, edrophonium acts as an in vivo activator of the ACh-receptor, which points to the usefulness of this membrane preparation for preclinical screening of myASThenia drugs.

TL;DR: Cell viability, energetic state, and metabolic function was investigated by Trypan Blue exclusion, by determination of cellular contents of sodium, potassium, and ATP, as well as by measurements of oxygen consumption.

TL;DR: The procedure was developed as a simple means of detecting increased levels of “free” protoporphyrin in capillary blood samples from young children with iron deficiency or increased lead absorption or both, and gives reproducible results in the hands of persons without prior formal training in laboratory skills.