Biomolecular Detection and Quantification 

TL;DR: It is shown that MinION sequence reads can enhance contiguity of de novo assembly when used in conjunction with Illumina MiSeq data, as the first nanopore-based single molecule sequencer available to researchers.

TL;DR: Recommendations for the precise estimation of PCR efficiency are proposed: one robust standard curve with at least 3–4 qPCR replicates at each concentration shall be generated, and using a larger volume when constructing serial dilution series reduces sampling error and enables calibration across a wider dynamic range.

TL;DR: This work presents methods to determine the limit of detection (LoD) and thelimit of quantification (LoQ) as applicable to qPCR, based on standard statistical methods as recommended by regulatory bodies adapted toqPCR and complemented with a novel approach to estimate the precision of LoD.

TL;DR: Recent advancements are explored and the current gaps in knowledge concerning each point of contact between cfDNA analysis and the different stages of cancer management are highlighted.

TL;DR: An overview of the main steps in the primer design workflow is presented, with data that illustrate some of the unexpected variability that often occurs when theory is translated into practice.