Showing papers in "BioNanoScience in 2022"
TL;DR: Liposomes are well-recognized and essential nano-sized drug delivery systems and have been employed as artificial cell models to mimic structure and functions of cells and are of immense use in various biological analyses as discussed by the authors .
Abstract: Liposomes are well-recognized and essential nano-sized drug delivery systems. Liposomes are phospholipid vesicles comprised of cell membrane components and have been employed as artificial cell models to mimic structure and functions of cells and are of immense use in various biological analyses. Liposomes acquire great advantages and provide wide range of applications as useful drug carriers in pre-clinical and clinical trials. This review summarizes exclusively on scalable techniques for liposome preparation and focuses on the strengths and limitations with respect to industrial applicability. Also, this review discusses the updated recent advancements in biomedical applications with a mention of key highlights of commercially available formulations, clinical trials and patents in recent past. Furthermore, this review also provides brief information of the classification, composition and characterization of liposomes.
35 citations
TL;DR: Liposomes are well-recognized and essential nano-sized drug delivery systems and have been employed as artificial cell models to mimic structure and functions of cells and are of immense use in various biological analyses as mentioned in this paper .
Abstract: Liposomes are well-recognized and essential nano-sized drug delivery systems. Liposomes are phospholipid vesicles comprised of cell membrane components and have been employed as artificial cell models to mimic structure and functions of cells and are of immense use in various biological analyses. Liposomes acquire great advantages and provide wide range of applications as useful drug carriers in pre-clinical and clinical trials. This review summarizes exclusively on scalable techniques for liposome preparation and focuses on the strengths and limitations with respect to industrial applicability. Also, this review discusses the updated recent advancements in biomedical applications with a mention of key highlights of commercially available formulations, clinical trials and patents in recent past. Furthermore, this review also provides brief information of the classification, composition and characterization of liposomes.
32 citations
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TL;DR: This is the pioneering report on the study of antibacterial and antioxidant activity of AaAgNPs, synthesized from endophytic fungus, Alternaria alternata of D. falcata, which have shown significant antibacterial property.
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TL;DR: In this paper , the authors reported biosynthesis of O/W (oil/water) nanoemulsions based on essential oil from Cymbopogon pendulus and analysis of its biological activities.
Abstract: Essential oil from Cymbopogon pendulus is immensely useful in various sectors like food, pharmaceutical, and cosmetic industries. Since this oil is hydrophobic, unstable, and volatile, hence encapsulation by using nanoemulsions technology is the best way to protect it. This study reports biosynthesis of O/W (oil/water) nanoemulsions based on essential oil from Cymbopogon pendulus and analysis of its biological activities. O/W nanoemulsions were prepared by using tween 20/80, sodium dodecyl sulphate as surfactants, and ethanol as co-surfactants. Fingerprinting of nanoemulsions using UV, fluorescent, and FT-IR was studied along with other parameters like pH and conductivity. Biological activities like antibacterial, anti-inflammatory, and anti-diabetic activities and drug release pharmokinetics were evaluated. Ethanol containing nanoemulsions was noticeably smaller than other nanoemulsions. Encapsulation efficiency of nanoemulsions was in the range from 41 to 60%. Nanoemulsions were spherical in shape and stable even after 50 days of storage. Appreciable biological activities like anti-bacterial, anti-inflammatory, and anti-diabetic activities were detected. Drug kinetic study revealed that nanoemulsions exhibited Korsmeyer-Peppas model. Based on this, the possible role of lemon grass oil-based nanoemulsions in cosmetic, food, and pharma sectors has been discussed.The online version contains supplementary material available at 10.1007/s12668-022-00964-4.
TL;DR: In this paper , the authors present a review of different applications of nanosponges and also mention about the recent SARS-CoV-2 management using nanosponge.
Abstract: Nanotechnology has a versatile use in the field of disease therapy, targeted drug delivery, biosensing, and environmental protection. The cross-linked nanosponges are one of the types of nanostructures that provide huge application in the biomedical field. They are available up to the fourth generation and can act as a payload for both kinds of hydrophilic and hydrophobic drugs. There are different methods available for the synthesis of these nanosponges as well as loading the drugs inside them. A variety of approved drugs based on nanosponges are already in the market including drugs for cancer. Other applications include the uses of nanosponges as topical agent, in improving solubility, as protein carrier, in chemical sensors, in wastewater remediation, and in agriculture. The present review discusses in detail about different applications of nanosponges and also mentions about the recent SARS-CoV-2 management using nanosponges.
TL;DR: In this paper , four different in-house probiotic isolates, i.e., Lactobacillus pentosus S6, Lactibacillus plantarum F22 and Lactebacillus crustorum F11, were used in the current study to check their ability to synthesize silver nanoparticles, showing maximum surface plasmon resonance (SPR) at a peak of 450 nm.
Abstract: Abstract The present work focusses on development of a safe, inexpensive, and more accessible source for biosynthesis of silver nanoparticles. Four different in-house probiotic isolates, i.e., Lactobacillus pentosus S6, Lactobacillus plantarum F22, Lactobacillus crustorum F11, and Lactobacillus paraplantarum KM1 isolated from different food sources, were used in the current study to check their ability to synthesize silver nanoparticles. All the probiotic-synthesized silver nanoparticles show maximum surface plasmon resonance (SPR) at a peak of 450 nm, which confirms the formation of silver nanoparticles. Scanning electron microscopy (SEM) analysis identified the shape and distribution of silver nanoparticles. Transmission electron microscopy (TEM) revealed the average size of synthesized nanoparticles in the range of 10–50 nm, with the smallest size of 5 nm for silver nanoparticles synthesized by L. crustorum F11. Further, Fourier-transform infrared spectroscopy (FTIR) detected the presence of different functional groups responsible for reduction of silver ion to form silver nanoparticles. The antimicrobial activity of these AgNPs was also found to be effective against different bacterial and fungal pathogens, viz., antibiotic-resistant Staphylococcus aureus , Bacillus cereus , Listeria monocytogenes , Pythium aphanidermatum , Fusarium oxysporum , and Phytopthora parasitica. However, L. crustorum F11–synthesized AgNP showed maximum inhibition against all the bacterial and fungal pathogens, with highest against S. aureus (20 ± 0.61 mm) and F. oxysporum (23 ± 0.37). Findings from this study provide a durable and eco-friendly method for the biosynthesis of silver nanoparticles, having strong antimicrobial activity against different multidrug-resistant microorganisms. Graphical abstract
TL;DR: In this article , the fabrication of nickel oxide nanoparticles (NiO-NPs) using the leaf extract of Ziziphus spina-christi L. and evaluating its potential biological activities.
Abstract: Abstract Over the past several years, the greener fabrication of metal oxide nanoparticles has attracted significant attention due to their simplicity, eco-friendliness, availability, and nontoxicity. This paper focused on the fabrication of nickel oxide nanoparticles (NiO-NPs) using the leaf extract of Ziziphus spina-christi L. and evaluating its potential biological activities. The characterization of synthesized NiO-NPs was confirmed using ultraviolet–visible spectroscopy, field emission-scanning electron microscope, energy-dispersive X-ray spectroscopy, and X-ray diffraction. Furthermore, protoscolicidal, antibacterial, and antioxidant activities and hemocompatibility of NiO-NPs were investigated. The findings revealed that the NiO-NPs were crystalline on nanoscale between 50- and 90-nm particle sizes. The NiO-NPs showed high scolicidal activity against Echinococcus granulosus . The viability of the treated protoscoleces exponentially decreased with an increase in the concentration of NiO-NPs. The NiO-NPs exhibited effective antibacterial activity against Escherichia coli and Staphylococcus aureus . NiO-NPs also possess a H 2 O 2 scavenging activity in a dose-dependent manner. This study revealed that the Z. spina-christi L. leaf extract is an effective reducing and capping agent for the production of NiO-NPs; it showed critical biological properties. Moreover, NiO-NPs have a potent antioxidant activity and low toxicity on the erythrocytes and appear hemocompatible.
TL;DR: The developed nanoliposomes may be considered as a promising anticancer formulation, which needs further clinical investigations and may provide a new direction to the cancer clinics.
TL;DR: In this paper , the SH-SY5Y neuroblastoma cell-derived membrane vesicles, obtained using cytochalasin B (CIMVs), with human bone marrow-derived MSCs were analyzed using imaging flow cytometry.
Abstract: At present, there is an increasing interest in the potential role of extracellular vesicles (EVs), acting as multi-signal messengers of the tumor stroma, in the development and progression of tumor. Tumor cell-derived EVs are considered a potential vector for the targeted delivery of antitumor agents due to the ability to fuse with parental cells through endocytosis and release their contents into the cytoplasm of the recipient cell. Tumor cell-derived EVs could be also used for priming immune cells and therapeutic vaccine development. It is also known that mesenchymal stem cells (MSCs) have a tropism toward tumor niches. It is believed that MSC migration to the tumor is due to its inflammatory signaling. Presumably, with the accumulation of MSCs at tumor sites, these cells differentiate into pericytes or tumor-associated fibroblasts, thereby forming a supporting tumor growth microenvironment. However, besides the ability to promote tumor progression, MSCs can also suppress its growth by inhibiting proliferation and cell cycle progression, and angiogenesis. Thus, the further studies of the MSC role in TME and MSC interaction with other cells of the tumor stroma, including through EVs, are of particular interest. To increase the yield of vesicles the isolation method based on pharmacological disorganization of the actin cytoskeleton induced by treating with cytochalasin B was used in this study. In this investigation the interaction of SH-SY5Y neuroblastoma cell-derived membrane vesicles, obtained using cytochalasin B (CIMVs), with human bone marrow-derived MSCs was analyzed using imaging flow cytometry. Using transmission electron microscopy, it was shown that CIMVs have a size similar to that of natural microvesicles, which is 100–1000 nm. Using imaging flow cytometry, it was shown that after 24 h of co-cultivation 6% of the MSCs contained a large number of CIMVs, and 42% of the MSCs contained a small amount of CIMVs. Cultivation of MSCs with SH-SY5Y cell-derived CIMVs also induced dose-dependent decrease in the expression of CD markers typical for MSCs. Thus, the internalization of SH-SY5Y cell-derived CIMVs within MSCs and the ability of the CIMVs to modulate immunophenotype of the recipient cells were shown. However, further studies are required to determine the effect of CIMVs on pro- or antioncogenic phenotype and function of MSCs.