Showing papers in "Cell Biology International Reports in 1981"

TL;DR: In this paper, a simple procedure is described for the production of 5 nm colloidal gold/secondary antibody reagents, which can be used for high resolution ultrastructural localization studies and this can be done with retention of satisfactory preservation of cell structure.

TL;DR: An analysis of the migration of fibroblasts in varied gradients of the 160 K peptide shows that the effect is chemotactic, i.e. directed migration of cells towards a higher concentration of the peptide.

TL;DR: A total of 1169 [35S]-methionine labelled polypeptides (855-acidic (IEF) and 314 basic (NEPHGE) from 100 asynchronous human HeLa cells have been separated using high resolution two dimensional gel electrophoresis.

TL;DR: Exocytosis implies the fusion of a secretory vesicle with the cell membrane and the temporary formation of a membrane continuum, which allows for the discharge of the secretory contents without interruption of the cell boundary.

TL;DR: The close similarities between this system and flagellar surface motility of Clamydomonas studied by particle movement and gliding motility are discussed.

TL;DR: Using a sensitive laser immunonephelometric procedure, an exponential increase of human plasma fibronectin (cold insoluble globulin) was shown to occur with age, with an average value between 0 and 50 years and between 70 and 90 years.

TL;DR: DM4 allows exponential growth of CCl 39 cells plated in serum-coated dishes without any lag and with a generation time of 15 hours, which is of great value to approach the mechanisms of escape of growth control by these cells.

TL;DR: By using one dimensional peptide mapping it has been possible to find homologies between several major mouse and HeLa cell polypeptides.

TL;DR: The results indicate that TRH is located specifically within the dermal glands of these species, and that both TRH and 5-HT are discharged from these glands following adrenergic stimulation.

TL;DR: Ammonium chloride (NH4Cl) was found to markedly inhibit the ability of cultured human fibroblasts to establish an antiviral state following exposure to poly IC.

TL;DR: The light-induced decrease in frequency can be used for experimental phase shifting, e.g., when studying the nature and the pathway of signal transmission for the spatial phase synchronization of contractile activities.

TL;DR: The results indicate that DFMO brings about a significant decrease in the intracellular levels of putrescine and spermidine followed by an inhibition of cell growth in both the normal and transformed cells, and the use of inhibitors like DFMO might help in designing better therapeutic regimens in combination with other cytotoxic drugs.

TL;DR: The results suggest that disassembled tubulin is preferentially associated with cytoplasmic microtubules and possibly other formed elements of the cytoskeleton.

TL;DR: The first local appearance of light response in non-illuminated parts of the plasmodia was used to analyse the sensory pathway of the light stimulus modulating the contractile apparatus and revealed that the direction of photosensory transduction is determined by the momentary direction of protoplasmic shuttle streaming.

TL;DR: Using colloidal gold as an electron dense marker, evidence is presented for the in vitro transport of rat IgG across the 11.5 day rat yolk sac endoderm, which was receptor mediated and involved a population of coated vesicles.

TL;DR: Curved micro Filamentous structures, termed arcs, are frequently found in the leading lamellae of cultured fibroblasts and provide clues to the organisation and regulation of the microfilament system for movement.

TL;DR: Using these precautions several monolayer cell lines can be grown routinely in milk supplemented media, just as well as in serum supplemented media.

TL;DR: It is proposed that (i) cell division does not have any regulatory function, and (ii) DNA synthesis may, indeed, have some affect on the synthesis of initiator.

TL;DR: Autoradiography showed that the syncytium is derived predominantly from the migration of fetal binucleate cells and not from the maternal uterine epithelium as most recent reports have suggested, and is similar to that reported in certain other eutherian mammals.

TL;DR: Self generated electric currents were studied in protoplasmic drops and small plasmodia of Physarum polycephalum with the aid of an extracellularly measuring vibrating electrode, pointing toward large changes in membrane arrangement or properties occurring during development of plas modia from protoplasmsic drops.

TL;DR: The results suggest that the polarisation and crawling-type movements represent a fixed action pattern which is performed independently of adhesion to the substratum.

TL;DR: PK 1 cells enter prophase and complete mitosis at 24–25°C but are inhibited from entering prophase at 20–21°C, which results in a temperature-induced prophase-interphase-prophase transition which can be repeated several times on the same cell.

TL;DR: The shedding of membrane vesicles from the tumor cell surfaces results in a significant reduction in the cell size and can be inhibited by 2-deoxyglucose, sodium azide and 2-4-dinitrophenol.

TL;DR: With the phosphoproteins, α -casein and phosvitin, the same golden-brown to yellow color was produced that was previously found with the nucleolar organizer region (NOR) specific proteins, B23 and C23, of Novikoff hepatoma cells.

TL;DR: It is demonstrated that secretory granule membrane is removed from the cell surface by endocytosis and subsequently major components are incorporated into the membrane of intact intracellular chromaffin granules.