Showing papers in "Chinese Journal of Biotechnology in 1993"

TL;DR: Three independent transgenic plants were selected, which were then propagated in the nursery field and shown to be toxic to two lepidopteran pests, Lymantria dispar L. and Apocheimia cinerarius Erschoff.

TL;DR: E. coli plasmid pCGN1055 containing the hygromycin phosphotransferase (Hpt) gene under the control of the plant promoter was introduced into wheat protoplasts by cationic liposome (lipofectin)-mediated transformation, and transgenic albino plantlets were obtained.

TL;DR: The first report on the successful oocyte final maturation in vitro in zebra fish is reported, giving grounds for the further investigation on the transfer of foreign genes in the germinal vesicles of the oocytes.

TL;DR: Successful transformation of plasmid DNA and transfection of phage DNA into E. coli were described by using intense electrical field of exponential decay waveform (electroporation) generated by a Gene Pulsar LN-101, easier, more time-saving and more efficient than Ca(2+)-dependent transformation (transfection) method.

TL;DR: The 3'-terminal genomic region of the Beijing isolate of soybean mosaic virus (SMV-BJ) has been cloned through technique of polymerase chain reaction (PCR) and it has been found that the SMV coat protein gene is expressed in E. coli by western blot analysis.

TL;DR: With ABI370A Autosequencer, the total nucleotide sequence of plasmid pXZ10145 from Corynebacterium glutamicum 1014-6T has been determined using the dideoxy chain termination method and a seven-base pairs sequence "ATCTAGC" was found.

TL;DR: Protoplasts from the leaf of Ichang papeda were fused with the protoplasts of embryogenic suspension culture of Valencia orange in vitro by polyethylene glycol (PEG)-induced fusion and these tetraploids were somatic hybrids that grew vigorously after transplanted into soil.

TL;DR: The beet necrotic yellow vein virus (BNYVV) isolate NM was isolated from sugarbeet infected with rhizomania in Inner Mongolia of China and its complete nucleotide sequence was determined by means of Sanger's dideoxy-mediated chain-termination method.

TL;DR: Based on the highly conserved region of human, rabbit and mouse SRY (sex-determining region Y) sequences, primers were designed for the amplification of the unknown homologous sequence of cattle by polymerase chain reaction (PCR), and a 200-bp male-specific amplifying fragment was obtained.

TL;DR: Western blot analysis showed that molecular weight of secreted recombinant pro- UK was the same as that of natural pro-UK, which is 52 kDa, and more than 60% of expression production was single chain urokinase (rscUK) without protease inhibitor in medium.

TL;DR: The plasmid pJG202 containing Tac promoter, calf prochymosin B(1-161) gene and human proinsulin gene was constructed and transformed into E. coli JM105 and humanProinsulin with native properties, as evidenced by amino acid composition analysis, receptor binding and radioimmunoassays, could be obtained.

TL;DR: The batch fermentation of L- arginine using Corynebacterium crenatum L-arginine-producing mutant 971.1 was kinetically studied in a 2.6 L fermentor and the kinetic fermentative behavior of the strain 971., related to the velocity and effectiveness of the metabolism of the cell, was mathematically analyzed.

TL;DR: Preliminary cloning system has been established on the basis of studies on the conditions for protoplast formation, regeneration, as well as DNA transformation for Y3 mutant strain Streptomyces cattleya, and southern hybridization confirmed that the transformant No. 12 harbors the recombinant plasmid p6BC12.

TL;DR: The biological assay revealed that the hybrid plasmid pMGC001 containing the Stx gene could produce Shiga toxin, and polyclonal and monoclonal antibodies against B subunit showed that they could react specifically to the B sub unit.

TL;DR: Full-length cDNA encoding rice (Oryza sativa, cv. Nongken 58S) calmodulin mRNA has been cloned using polymerase chain reaction (PCR) with synthesized primers from the sequence of barley CaM gene.

TL;DR: Human prourokinase (pro-UK) cDNA without the signal peptide was obtained using synthetic oligonucleotide and DNA recombination techniques and was successfully expressed in E. coli.

TL;DR: The batch experiment results showed that the calcium alginate was the best one of the five immobilized materials and the observed productive acid rate and its yield of immobilized cells appeared to be greater than that of the corresponding free cells suspension.

TL;DR: Good relationship was observed between on-line and off-line sampling for glucose determination in yeast fermentation, the correlation coefficient r was 0.985 and glucose penetration rate Rp was stable for at least 48 hr.

TL;DR: The Northern blot analysis and slot blot analysis of RNA isolated from leukemic cell lines and blast cells of acute leukemia showed that MPO gene expression correlated with myeloid lineage and might be used as a marker for the subclassification of acuteukemia.

TL;DR: The oxygen transfer rates were investigated systematically in the CellCul-20A bioreactor with the device of cage aeration and the oxygen transfer coefficients of surface and deep aerations were correlated, based on the experimental data.

TL;DR: In fed-batch culture, the growth rate of W3110 (pEC901) was maintained at a high level by controlling the feeding rate of glucose, and the interferon titer reached 2.5 x 10(10) u/l, which was greatly improved compared with those reached in shaker flask cultures.

TL;DR: This paper using the synthesis of mung bean trypsin inhibitor gene as an example, presented a new method for gene synthesis based on the combination of the single strand strategy and the PCR technique that was very simple, convenient and rapid.

TL;DR: Through observation of the morphology, the size, the DNA content and the activity of glucoamylases, the differences between the fusants and segregants and their parental strains were shown and indicated the new possibility for strain improvement in A. niger.

TL;DR: Rhizobium meliloti 042B is a fast-growing, salt-tolerant and high efficiency nitrogen-fixing symbiont with alfalfa, and transconjugants which tolerated and fixed nitrogen efficiently in soybean were constructed.

TL;DR: In the search for biocompatible extractants for extractive fermentation of lactic acid, the effect of twelve organic solvents on the activity of L. delbrueckii were studied at different concentration levels and the compatibilities were summarized into four classes: completely compatible, compatible at molecular level, partially compatible and non-compatible.

TL;DR: Evidence that specific molecular interactions occur between three nod regulation elements: naringenin, NODD and nod-promoter is presented.

TL;DR: In this article, photosynthetic light reaction occured in the thylakoid membrane of chloroplast and PSI is one of the important reaction centers converting light energy to the chemical energy.