Showing papers in "Gene in 1982"

TL;DR: A series of plasmid vectors containing the multiple cloning site (MCS7) of M13mp7 has been constructed and a kanamycin-resistance marker has been inserted into the center of the symmetrical MCS7 to yield a restriction-site-mobilizing element (RSM).

TL;DR: Two new M13 vectors, M13MP8 and M13mp9, have been constructed that permit the cloning of the same restriction fragment in both possible orientations, allowing the use of only one of the two DNA strands as a template for M13 shotgun sequencing.

TL;DR: The nucleotide sequence of 1200 bp from the unique region of transposon Tn5 containing the neomycin phosphotransferase gene (neo) was determined, and the location of the neo gene was identified by deletion mutants in a translational reading frame of 792 bp.

TL;DR: A cosmid derivative of the low copy-number broad host-range cloning vector pRK290, pLAFR1 is constructed, is 21.6 kb long, confers tetracycline resistance, contains a unique EcoRI site, and can be mobilized into and stably replicates within many Gram-negative hosts.

TL;DR: It is hypothesized that both the optimization of codon-anticodon interaction energy and the adaptation of the population to codon frequency or vice versa in highly expressed mRNAs of E. coli are part of a strategy that optimizes the efficiency of translation.

TL;DR: A novel approach has been developed for the preparation of highly radioactive, strand-specific M13 probes by using a universal primer to initiate the DNA synthesis of the complementary strand of the M13 sequence downstream from the inserted sequence.

TL;DR: Two small low-copy-number plasmid vectors were constructed by in vitro and in vivo recombinant DNA techniques, and carry genes conferring resistance to tetracycline and kanamycin, and should be useful for cloning many genes coding for membrane and regulatory proteins which cannot be cloned into high-copy number plasmids.

TL;DR: A system based upon the activity of the Tn 9 Cm r gene whose product, chloramphenicol acetyltransferase (CAT), can be easily assayed enzymatically and identified phenotypically in Escherichia coli and it should be possible to study gene expression on any extrachromosomal element in the appropriate host.

TL;DR: The gene coding for alpha-amylase from Bacillus amyloliquefaciens was isolated by direct shotgun cloning using B. subtilis as a host and revealed to be present in a 2.3-kb insert, which was shown to be indistinguishable from that of B. amylliquefeciens as based on immunological and biochemical criteria.

TL;DR: A plasmid that is able to replicate in both Escherichia coli and Streptococcus sanguis has been constructed by the in vitro joining of the pACYC184 (Cmr Tcr) and pVA749 (Emr) replicons.

TL;DR: Construction of a versatile Streptomyces cloning vector (pIJ61) is reported, which carries neomycin phosphotransferase and thiostrepton resistance genes and has unique BamHI and PstI sites which will allow clone recognition by insertional inactivation ofNeomycin resistance.

TL;DR: The structural gene of the sweet-tasting plant protein (prepro)thaumatin was cloned and expressed in Escherichia coli and expression was effected under control of lac and trp promoter/operator systems and through the use of bacterial ribosome-binding sites.

TL;DR: Analysis of activity of related promoters with differences in spacing indicated that a distance of 19 bp yields a very weak promoter, and that 18 bp is less active than the 17-bp spacing, which is the most frequently found spacing in promoters.

TL;DR: A complete gene library was established in a set of clones containing the viral DNA in long overlapping segments in partially cleaved and cloned in the respective cleavage sites of cosmid pHC79.

TL;DR: The gene for the large subunit (LS) of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCase/Oase) from tobacco has been cloned in pBR322 and sequenced and the deduced amino acid sequence of tobacco LS protein shows 90% homology with those of maize and spinach LS.

TL;DR: Analysis of the 5′ end of the mRNA for another VSG (221) whose gene is thought to be activated by a different mechanism to that of VSGs 117 and 118 indicates that the 5″- most 35 nucleotides of the VSG 221 mRNA are identical to the 117/118 mini-exon sequence.

TL;DR: The 4150-bp plasmid pBR329 was constructed by the the insertion into pBR327 of an 877-bp DNA fragment carrying the Cmr gene from pBR328, which lacks its original promoter but is transcribed counterclockwise toward the Apr gene by a promoter located to the right of the HindIII site in the Tcr gene.

TL;DR: Functional sub-regions within the HIS4 coding frame have been identified by determining the sequence changes for various his4 mutations by identifying the 5' and 3' flanking sequences.

TL;DR: Methods for growing phage, preparing single- and double-stranded DNA, and cloning are given in the "cook-book" form, to minimize the practical problem often associated with filamentous-phage cloning.

TL;DR: The plasmid pHV32, which replicates in Escherichia coli but not in Bacillus subtilis, transformed B. subtil is-competent cells efficiently when linked in vitro to EcoRI B. Substantial, and carried pHV 32 inserted in their chromosomes, and often displayed a mutant phenotype.

TL;DR: Computer analysis of the DNA sequences revealed that there are up to fifteen open reading frames which could encode polypeptides containing more than thirty amino acids and a number of potential regulatory signals, promoters and ribosome binding sites.

TL;DR: Use of phi C31 lysogenic recipient permitted the integration of the att-deleted phage, presumably by homologous recombination, giving tetracycline-resistant double lysogens.

TL;DR: The large stem and loop structure can be constructed from the sequences surrounding the 5' and 3' ends of the 16S gene, demonstrating the prokaryotic nature of chloroplast 16S rRNA.

TL;DR: The transposon Tn5 contains a unique central region bordered by 1.5-kb inverted repeats which is unstable on subsequent transformation into Escherichia coli (Collins, 1981).

TL;DR: A sensitive and simple immunoassay was developed to screen Escherichia coli transformed with recombinant DNA plasmids carrying a cellulase gene and the enzyme present in extracts of E. coli carrying the plasmid was active in catalysing the hydrolysis of carboxymethylcellulose.

TL;DR: A synthetic 17-mer corresponding to the deduced sequence was shown to hybridize strongly to a 9-kb HindIII fragment from N. crassa wild-type DNA but not to any corresponding fragment from the DNA of a mutant strain known to be deleted for most or all of the gene.

TL;DR: A new plasmid cloning vector (pHE3) is described carrying dominant p-fluorophenylalanine-sensitivity (pheS) and chloramphenicol-resistance markers, which can be cloned into pHE3 leading to insertional inactivation of pheS.

TL;DR: A family of plasmids containing short pieces of Escherichia coli lac promoter DNA has been constructed to serve as convenient sources of lac DNA fragments containing the 'up' promoter mutations UV5 or Ps (super promoter) as well as the wild-type promoter.

TL;DR: A phage lambda cloning vector has been constructed which contains a single site for the restriction endonuclease BamHI, and is used to construct an Escherichia coli library using partial digestion with Sau3A.

TL;DR: Baby rat kidney cells were transfected either with intact region E1 DNA of adenovirus type 5 (Ad5) or with mixtures of DNA fragments containing the separated E1a and E1b regions, indicating that the degree of oncogenicity is determined by region E 1b.