Showing papers in "Journal of Chromatography B in 2010"

TL;DR: Results show that straight aldehydes may be promising biomarkers associated with NSCLC, and increase the sensitivity and specificity of previously identified VOC patterns.

TL;DR: A validated method for the detection and quantification of naphthalen-1-yl-(1-pentylindol-3-yl)methanone (JWH-018), an ingredient of a herbal mixture called "Spice", by means of HPLC-ESI-MS-MS in serum is described.

TL;DR: A simple and economical high performance liquid chromatography method was developed and validated for routine analysis of 12 Penicillin, Cephalosporin and Carbapenem antibiotics in 200 microL of human plasma and has been used in a pathology laboratory for therapeutic drug monitoring (TDM) of beta-lactams in critically ill patients.

TL;DR: HILIC method transfer guidelines were shown to further enhance the resolution of glycans by changing initial gradient conditions, flow rate, column temperature, and different column lengths, achieving excellent resolution in the separation of 2-AB labeled glycans.

TL;DR: In this work an in vitro multi-step static model to simulate complete digestion of the bovine milk proteins has been developed, and casein and whey protein peptides which survived the in vitro GI digestion have been identified by the combined application of HPLC and mass spectrometry techniques.

TL;DR: Investigation of potential matrix effects resulting from different endogenous lipid classes, including phospholipids, acylglycerols and cholesterols, in order to establish a library for the relative presence of these components in biological sample extracts obtained by commonly used sample preparation techniques, and a simple LC/MS/MS method was developed.

TL;DR: This LC-MS/MS method is reliable and robust for the measurement of both 25- OH vitamin D3 and 25-OH vitamin D2 in human serum and is compared to the DiaSorin radioimmunoassay (RIA) and a recently re-standardised version of an automated electrochemiluminescent immunoassays (ECLIA) from Roche Diagnostics.

TL;DR: This review provides a critical assessment of the applications of immunoaffinity columns for sample clean-up in the field of food safety, comparing extraction methods, achievable recovery, and illustrating the variety of end-detection methods that have been employed.

TL;DR: A simple, fast and sensitive HPLC-MS/MS method for determination of the commonly used protease inhibitors, as well as the more recent antiretrovirals, the CCR5 antagonist maraviroc and the "second generation" NNRTI etravirine and rilpivirine is described.

TL;DR: The plasma procedure has been successfully applied in the quantitative determination of selected analytes in samples collected from nicotine-abstinent human participants as part of a pharmacokinetic study investigating biomarkers of nicotine use in plasma following controlled low dose (7 mg) transdermal nicotine delivery.

TL;DR: Indoxyl sulfate is the best indicator of the effect of AST-120 in CRF rats, and 4-Ethylphenyl sulfates is a newly identified uremic substance.

TL;DR: This method was successfully applied to determine plasma concentrations following administration of ticagrelor in human volunteers and patients, and animal safety evaluation studies and human incurred samples were found to generate consistent reproducibility and stability results.

TL;DR: A method that allows analysis of OTA and its detoxication product ochratoxin alpha (OTα) in urine and in blood plasma is developed and it is proposed to include analyses of this metabolite in future biomonitoring studies, also in light of the observed variations for urine OTα-levels that suggest different interindividual abilities for OTA-detoxification in humans.

TL;DR: Palmitic acids, stearic acid, oleic acid , linoleic acid and α-linolenic acid were identified as potential biomarkers for distinguishing IPD from healthy control and T2DM individuals, which might be helpful for diagnosis and characterization of diabetes.

TL;DR: A rapid, sensitive and flexible LC/MS/MS method has been developed and validated to measure voriconazole, fluconazole (FLZ), itraconaz Molecule (ITZ) and posaconazoles (PSZ) in human serum, suitable for clinical pharmacokinetic studies and routine monitoring in daily practice.

TL;DR: This method was satisfactorily applied to the determination of BPA and its chlorinated derivatives in 49 placental tissue samples collected from women who live in the province of Granada (Spain).

TL;DR: The simultaneous measurement of endogenous and exogenous glucocorticoids contributed to patient care in cases with dexamethasone and metyrapone dynamic tests and unsuspected therapeutic glucoc Corticoid use.

TL;DR: A fast, sensitive, universal and accurate method for the determination of four different tyrosine kinase inhibitors from biological material was developed using LC-MS/MS techniques and illustrated with the analysis of samples from a cellular accumulation investigation and from determination of steady state concentrations in clinically treated patients.

TL;DR: Comparison of the matrix effect of bile acid-free plasma with that of real plasma indicated that the charcoal purification procedure did not affect the properties of charcoal-purified plasma as calibration matrix.

TL;DR: A novel method is described for the determination of tramadol in biological fluids using molecularly imprinted solid-phase extraction (MISPE) as the sample clean-up technique combined with high-performance liquid chromatography (HPLC).

TL;DR: Recent advances in the determination of vitamin D metabolites and related compounds in biological samples using liquid chromatography coupled with mass spectrometry using LC-MS are reviewed.

TL;DR: The GC-MS method is suitable for use as a reference method for urinary creatinine in humans and can be extended to other biological samples such as saliva.

TL;DR: The new method for the simultaneous determination of ACh, betaine, choline, and DMG is sensitive, precise, and fast enough to be used in clinical investigations related to the methylation pathway.

TL;DR: This review describes reports of the enantiomeric separation of commercial organophosphorus pesticides (OPs) and the applications of these methods to research on theEnantioselectivity of the toxicity and environmental fate of these compounds.

TL;DR: This paper provides a short historical overview of the development of bioanalytical methods for chemical warfare (CW) agents and their biological markers of exposure, with a more detailed overview of methods for organophosphorus nerve agents.

TL;DR: Indoxyl sulfate was demonstrated to be the first principal serum metabolite which differentiates CRF from normal, followed by phenyl sulfATE, hippuric acid and p-cresyl sulfates, and these serum levels were markedly increased in CRF rats as compared with normal rats.

TL;DR: This validated method was successfully applied to a randomized, two-period cross-over bioequivalence study in 20 healthy Chinese volunteers receiving multicomponent formulations containing 325 mg of paracetamol, 30 mg of pseudoephedrine hydrochloride, 15 mg of dextromethorphan hydrobromide and 2 mg of chlorphenamine maleate.

TL;DR: A rapid and selective method was successfully developed using the magnetic molecularly imprinted polymer (MMIP) as sorbent for the extraction of β-lactam antibiotics (BLAs) from milk samples using PENV, amoxicillin and oxacillin in five milk samples from different provenances.

TL;DR: Two new methods using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the quantification of the major unconjugated bile acids in human serum are described, which provide information on the composition of the bile acid pool on one hand and on the relative amount of newly synthesised bile Acid metabolism, which taken together gives new insights in the investigation of bileacid metabolism.

TL;DR: A simple offline LC-MS/MS method for the quantification of sitagliptin in human plasma is described and precision and accuracy were assessed based on the analysis of six sets of calibration standards prepared in six lots of human control plasma.