Showing papers in "Journal of Forensic Sciences in 1995"

Obsessional harassment and erotomania in a criminal court population

Abstract: The criminal behaviors of harassment and menacing are difficult to control, and of increasing concern to the general public and local law enforcement officials. In 1992, the New York State Legislature modified the Penal Law, responding to public fears and concerns that stalking behavior may become violent. Some persons charged with these types of offenses are suffering from psychiatric disorders. Among these disorders are those classified as Delusional Disorders. According to both DSM-III-R (1987–1993) and DSM-IV (1994), there are five specific types of Delusional Disorder: erotomanic, grandiose, jealous, persecutory and somatic. This type of disorder tends to be chronic. Forty eight cases of persons charged with harassment and menacing in the New York County Criminal and Supreme Court and referred for evaluation to the Forensic Psychiatry Clinic between January 1987 and January 1994 are reviewed. When cases of erotomania and other affectionate/amorous complaints were compared with persecutory/angry forms of harassing behavior, there was a great deal of similarity. When all harassers were compared to the Clinic population as a whole, major differences in ethnicity, age, educational level and sex were noted. Findings are presented regarding incidence, other demographic data, recidivism, violence and clinical diagnosis. The researchers conclude that erotomania does exist, however, there are other psychiatric disorders which can also be diagnosed in individuals accused of harassing and menacing behavior. From the point of view of the victim and the criminal justice system, the similarities in behavior patterns are more important than the different diagnoses.

Allometric secular change in the long bones from the 1800s to the present

Abstract: Allometric secular changes in the six long limb bones for White and Black males from the mid 1800s to the present are examined. Long bone lengths are available from the Terry collection and WWII casualties. We conducted two types of analysis to reveal secular changes. First, allometry scaling coefficients were derived by regressing log bone length onto log stature. These showed that the femur, tibia and fibula were positively allometric with stature, while the humerus, radius and ulna were isometric. The lower limb bones were more positively allometric in the WWII sample than in the Terry sample. Second, secular changes in length of femur and tibia and in the tibia/femur ratio were evaluated, using modern forensic cases in addition to the Terry and WWII samples. This analysis shows that secular increase in lower limb bone length is accompanied by relatively longer tibiae. Secular changes in proportion may render stature formulae based on nineteenth century samples, such as the Terry collection, inappropriate for modern forensic cases. The positive allometry of the lower limb bones argues against using simple femur/stature ratio, which assumes constant proportionality, as an alternative to regression equations.

Combined homicide-suicides: a review.

Abstract: Although the rate of combined homicide-suicides is low compared with that for suicide alone or homicide, homicide-suicides generate much public concern. In some cases, the homicide-suicide involves annihilation of an entire family or multiple non-family members. A difficult phenomenon to study--in part because the perpetrator is dead--it is, nonetheless, crucial to attempt to advance our understanding of this tragic phenomenon from a psychiatric view. This literature review then addresses demographic variables; proposes two classifications, one based on psychopathology, the other on the relationship between offender and victim; and suggests a three dimensional analytical approach to understanding homicide-suicide: 1) psychopathology and ego deficits of the perpetrator, 2) cumulative and precipitating stressors, and 3) motivation and vector of destructive urges against self and the other victim(s). Finally, some implications for mental health clinicians and forensic experts are offered. In attempting to understand acts of homicide-suicide, inquiry into the following dimensions should be useful: Ego Weakness. What type of mental disorder(s), psychopathology, or personality traits may have contributed to the homicidal-suicidal behavior? Stressors. What type of acute and chronic stressors did the individual experience leading up to this act? Vectors. Whom did the individual select to kill and why? Were some victims more clearly primary and others secondary or incidental?

Differentiation of perimortem and postmortem trauma using taphonomic indicators.

Abstract: Skeletal remains discovered at a construction site in Georgia display classic "butterfly" fractures on several long bones. Although this fracture pattern is usually associated with perimortem trauma, in this case taphonomic indicators demonstrate that they can also occur on dry defleshed bone. Variations in bone color at the fracture sites indicate recent postmortem trauma. Analysis of the directions of force and points of impact indicates that the bones were most likely disarticulated when the trauma occurred.

Validation and Population Studies of the Loci LDLR, GYPA, HBGG, D7S8, and Gc (PM loci), and HLA-DQα Using a Multiplex Amplification and Typing Procedure

Abstract: Studies were performed to evaluate the forensic applicability of multiplex amplification of the loci low density lipoprotein receptor, glycophorin A, hemoglobin G gammaglobin, D7S8, and group-specific component (PM loci) and simultaneous typing of these loci using a reverse dot blot approach where allele specific oligonucleotide probes are immobilized on a nylon membrane strip. These results were obtained by using the AmpliType® PM PCR Amplification and Typing Kit. The experiments included: mixed body fluid studies; chemical contaminant effects on the DNA in body fluid samples; the effect of typing DNA from body fluid samples deposited on various substrates; the effect of microorganism contamination on typing DNA derived from blood and semen; the effect of sunlight and storage conditions on DNA typing; determination of the sensitivity of detection of the PM test kit; determination of cross-reactivity of DNA from species other than human; typing DNA derived from various tissues from an individual; and an evaluation of the hybridization temperature of the assay. The data demonstrate that DNA exposed to a variety of environmental insults yields reliable PM typing results. Allele and genotype frequencies for six loci (PM loci and HLA-DQα) were determined in African Americans, Caucasians, southeastern Hispanics, and southwestern Hispanics. All loci meet Hardy-Weinberg expectations and there is little evidence for association of alleles between the loci. The frequency data can be used in forensic analyses and paternity tests to estimate the frequency of a multiple locus DNA profile in various general United States populations.

Offender and Offense Characteristics of Sexual Sadists: A Comparative Study

Abstract: This paper compares the offender and offense characteristics of 30 sexually sadistic criminals studied by Dietz et al. with those of 29 sexually sadistic criminals and 28 nonsadistic sexual offenders from the Royal Ottawa Hospital. We examined whether the characteristics noted by Dietz et al. would be found among a less violent group of sadistic offenders and also to what extent these characteristics were specific to sexual sadism. Results suggest some offender and offense characteristic are specific to sexual sadism and that others appear to be common to sexually aggressive offenders more generally. In addition, there were a number of characteristics found exclusively among the most violent offenders described by Dietz et al. An explanation of these results in terms of a biopsychosocial understanding of sexual sadistic offenders is provided.

Postmortem drug metabolism by bacteria.

Abstract: Studies were undertaken to determine the possible role of enteric bacteria in the postmortem bioconversion of the nitrobenzodiazepines flunitrazepam, clonazepam, and nitrazepam. Flunitrazepam, clonazepam, and nitrazepam were completely metabolized in blood in the presence of eight species of enteric bacteria to their respective 7-amino-metabolites. The rates of metabolism, at 37°C, ranged from 0.1 ng/mL/min for Streptococcus faecalis to 8.8 ng/mL/min for Clostridium perfringens. The rate of conversion was reduced to 87% by a combination of 0.7% (w/v) sodium fluoride and potassium oxalate, and almost completely inhibited (96%) by 1% (w/v) sodium fluoride. pH had variable effects on the rate of metabolic bioconversion of nitrobenzodiazepines, while increasing temperatures were found to generally increase the rate of nitrobenzodiazepine bioconversion. These data support the proposal that bacteria may mediate postmortem bioconversion of the nitrobenzodiazepines.

Systematic Toxicological Analysis Using HPLC/DAD

Abstract: A high-performance liquid chromatographic method with diode-array detection (HPLC/DAD) for systematic toxicological analysis of human blood or plasma samples is presented. After single-step liquid/liquid extraction at pH 9.5 using chloroform/2-propanol/n-heptane (60:14:26, v/v/v), the drugs elute isocratically from a NovaPak C18 (Waters) 4-micrometers column (300 mm x 3.9 mm, i.d.) at 30 degrees C, with methanol/tetrahydrofuran/pH 2.6 phosphate buffer (65:5:30, v/v/v) as the mobile phase (flow rate 0.8 mL/min). Full UV spectra from 200 to 400 nm (resolution 1.3 nm) are recorded on-line during the 20 min chromatographic run. Solute identification may be automatically performed by comparison of analytical data (retention times and UV spectra) with references of 311 pharmaceuticals, toxicants and drugs of abuse stored in a computerized library. The method is simple, rapid, relatively inexpensive and highly specific. The previously reported applications of HPLC/DAD technology to drug screening are reviewed, and the interests and limitations of the method are discussed in the light of this literature.

Should We Estimate Biological or Forensic Stature

Abstract: Trotter and Gleser's stature regression equations were derived from partly incorrect measurements of long bones and antemortem measured statures (MSTATs). Forensic anthropologists have applied these equations to correctly measured bones and compared resulting estimates to a forensic stature (FSTAT), usually obtained from a driver's license. Forensic anthropologists have also used Trotter and Gleser's standard error as a stature prediction range, despite published warnings that it is not wide enough for this purpose. The combination of these factors has resulted in inaccurate and unrealistically precise estimates of stature from the long bones. Several factors decrease the accuracy of measured statures, and a reanalysis of Trotter's data reveals that estimating a biological stature is more imprecise than previously supposed. For FSTATs, these estimates are inaccurate as well. Using data from the Forensic Data Bank, new regression equations for predicting FSTAT were calculated, and in some cases are more precise than regressions based on Trotter's data using MSTATs. Confidence intervals for a single prediction, or prediction intervals, were calculated and are superior to standard errors for providing a range for stature estimations.

Elevated postmortem tryptase in the absence of anaphylaxis.

Abstract: Elevations in tryptase, a recently discovered mast cell enzyme, have been proposed as a postmortem indicator of fatal anaphylaxis. The previous studies had limited numbers of controls and thus the specificity of the test with postmortem samples was not known. Therefore, tryptase was evaluated in postmortem blood samples from 49 autopsy cases where there was no evidence of fatal anaphylaxis. The tryptase was above the normal serum threshold of 1 nanogram/mL (ng/mL) in 31 of these cases. Twenty-four cases had values in the 1 to 5 ng/mL range, two cases were between 5 and 10 ng/mL, and five were greater than 10 ng/mL. One autopsy specimen had a tryptase value of 106 ng/mL. The postmortem interval and the specimen storage condition did not appear to correlate with these elevations in tryptase. Although elevations in the postmortem tryptase remain an important supporting finding in the diagnosis of fatal anaphylaxis, it should not be used alone as the sole criterion for the postmortem diagnosis of anaphylaxis.

Fatality Due to Gamma-Hydroxybutyric Acid (GHB) and Heroin Intoxication

Abstract: The first case of fatal intoxication due to ingestion of gamma-hydroxybutyric acid (GHB) and intravenous use of heroin is reported. A 42-year-old man, known to have been a heroin addict and to have taken other psychoactive substances, who had been in treatment with GHB for several months, was found dead. Anatomohistopathologic examination showed generalized visceral congestion, edema and pulmonary anthracosis, chronic bronchitis and chronic active hepatitis. Toxicological findings included fluid and tissue distributions of GHB, morphine and 6-monoacetylmorphine. GHB and morphine concentrations were respectively 11.5 and 0.77 micrograms/mL (blood), 84.3 and 0.3 micrograms/mL (vitreous humor), 258.3 and 1.35 micrograms/mL (urine), 57.0 and 14.3 micrograms/mL (bile), 40.0 and 0.43 micrograms/g (brain), 43.0 and 0.60 micrograms/g (liver), 47.0 and 0.68 micrograms/g (kidney). Blood and urine levels of 6-monoacetylmorphine were 28.5 and 12.1 ng/mL respectively. The presumed mechanism of action and pharmacokinetics of GHB are briefly reviewed, with reference to its therapeutic use and to reports of non-fatal GHB intoxication.

Forensic DNA testing on skeletal remains from mass graves: a pilot project in Guatemala.

Abstract: A reliable method for extracting DNA from teeth was developed and successfully applied to a set of 12 skeletons recovered from two 10-year-old Guatemalan mass graves. Attempts to identify the remains by mitochondrial DNA (mtDNA) testing were hampered by low sequence diversity. Our findings demonstrate the feasibility of using DNA typing to identify victims from mass graves.

In situ detection and identification of trace explosives by raman microscopy

Abstract: The innovative design of the newly developed Renishaw Raman Microscope system and its application to the in situ detection and identification of plastic explosives contained in fingerprint samples are presented. Raman microscopy is a nondestructive inspection method. Our experimental results show that Raman spectra and Raman band images can be obtained from explosive particles as small as 1 µm3 in size or 1 picogram in mass. After exploring the full potential of the Raman microscopic technique, the aim of this research is to develop a real-time and field-deployable plastic explosive detection system.

Estimation of stature from the length of the cervical, thoracic, and lumbar segments of the spine in American whites and blacks.

Abstract: In order to estimate stature from the length of cervical, thoracic, lumbar, thoraco-lumbar (T-L) and cervico-thoraco-lumbar (C-T-L) segments of the spine, measurements were made on white and black Americans, both male and female, autopsied during 1977-1993. Sample sizes were as follows: white males = 167; white females = 58; black males = 43; black females = 31. Separate measurements were made of the vertebral segments along the anterior surface of the spine. Regression formulae were calculated for each segment in each of the four groups. Standard errors of estimate ranged from 2.60 to 7.11 cm. Comparison was made with previous work published for Japanese. The Japanese formulae could not predict stature of the American populations using our data. The method is useful for estimating the stature of severely burned or mutilated bodies.

Sex assessment from metacarpals of the human hand.

Abstract: Discriminant functions designed for the determination of sex from metacarpal measurements are presented. Three samples of metacarpal specimens were employed in the analysis; one consisting of 212 individuals from the Terry Collection, one of 33 individuals from the Royal Free Medical School in London, and finally, 40 individuals from the Forensic/Donated Collection, Maxwell Museum of Anthropology, University of New Mexico, all of whom had documented sex. Five measurements designed to characterize the size and shape of the human metacarpal were taken on all five digits. Based on the Terry Collection, significant metric differences attributed to race were found for digits I and III, and thus functions could only be derived for the three remaining metacarpals. Sex discriminant functions derived from the Terry Collection for digits II, IV, and V provide correct classification of 92.0, 86.26, and 84.37 percent. The resulting three linear equations were then independently applied to the Royal Free Medical School and Forensic/Donated samples to validate the accuracy of the original functions. Percentage of correct classification for each of the test samples varies.

Stages of epiphyseal union for thoracic and lumbar vertebral centra as a method of age determination for teenage and young adult skeletons

Abstract: Many current methods of age determination available to forensic anthropologists are limiting in that the age ranges provided are often broad, particularly for individuals in their late teens to early 20's. This study introduces an improved method for aging teenagers and young adults. The pattern and stages of union of the superior and inferior epiphyses of the vertebral centra (or ring epiphyses) were examined in 55 individuals, females and males, black and white, between ages 11 and 32 years. Vertebral ring epiphyseal union was found to be a good predictor of age. The correlation between stages of union and known age was 0.78 (P < .0001). The standard deviation was 2.566 years at the 99.9% confidence level. Sex differences were observed, but were not statistically significant. A larger sample size may perhaps demonstrate statistically significant differences in sex, and may or may not yield differences in race. A preliminary interobserver bias test showed high replicability. Results of this study compare favorably with results of other aging studies. Current age information for the progress of vertebral ring epiphyseal union is supplied for young males--and for the first time--females. This improved aging method provides necessary corroborative information for use with observations from other skeletal age indicators. Data collected from epiphyseal union of the vertebral centra aid in lessening the gap for early adult age determination.

The measure and mismeasure of the tibia : implications for stature estimation

Abstract: Trotter and Gleser's stature estimation formulae, based on skeletons of the Terry collection and on WWII casualties, have been widely used in forensic work. Our work with the Terry and WWII data yielded tibia lengths too short compared to other data sets. Using Trotter's original measurements, we discovered that she consistently mismeasured the tibia. Contrary to standard practice and her own definitions, she omitted the malleolus from the measurement. Trotter's measurements of the tibia are 10 to 12 mm shorter than they should have been, resulting in stature estimations averaging 2.5 to 3.0 cm too great when the formulae are used with properly measured tibia. We also examined tibia lengths of Korean War casualties, which were measured by technicians rather than by Trotter. Korean tibia measurements are also too short, but by a smaller amount than Terry and WWII. Since the Korean tibia are unavailable for restudy, it is unclear how they were measured. Estimation of stature from Trotter and Gleser's tibia formulae is to be avoided if possible. If necessary, the 1952 formulae could be used with tibia measured in the same manner that Trotter measured, excluding the malleolus.

Estimating maggot age from weight using inverse prediction

Abstract: Forensic entomological evidence is most often used to estimate the postmortem interval (PMI). Satisfactory techniques have not been available to quantify the precision of such a PMI estimate. For Cochliomyia macellaria (F.) (Diptera: Calliphoridae), we describe construction of a confidence interval on age of a larva, given its weight. The method requires a controlled experiment by which weights of larvae are observed at ages spread over sufficient range to cover the time from egg hatch up to postfeeding stage. A statistical model relating distributions of weights to age is formulated and fit to these data. We assumed a simple model in which both means and variances of weight distributions are linearly interpolated between sampled ages. The weight of a larva of unknown age is then compared to the fitted model via inverse prediction to compute the confidence interval on age of the larva.

Region Does Not Mean “Race”—Reality Versus Convention in Forensic Anthropology

Abstract: Norman Sauer has posed the rhetorical question: if races do not exist, how come forensic anthropologists are so good at identifying them? The simple answer is that, as members of the society that poses the question, they are inculcated into the social conventions that determine the expected answer. They should also be aware of the biological inaccuracies contained in that “politically correct” answer. Skeletal analysis provides no direct assessment of skin color, but it does allow an accurate estimate of original geographical origins. African, eastern Asian, and European ancestry can be specified with a high degree of accuracy. Africa of course entails “black,” but “black” does not entail African. The significant identifying features of a given region then are stochastically determined and are not the products of natural selection. If they are valuable for purposes of identification, they have no coherent adaptive, that is, biological, significance. Neither individual traits nor a configuration of them associated with a given region have any adaptive significance and thus have no comparative worth. Traits of adaptive value however are not constrained by region and cannot be used to identify “race.”

DNA analysis of dental pulp to link incinerated remains of homicide victim to crime scene.

Abstract: Teeth endure postmortem degradation and extreme changes in ambient temperature and pressure better than most human tissues. This ability to resist deterioration allows the teeth to be studied as a method of establishing the identity of a decedent. Additionally, dental hard tissues, and in some instances soft tissues, may provide investigators with other sources of forensic data. In this case, a female homicide victim was transported to a location where her remains were burned. The high temperatures of a gasoline fire effectively incinerated the body precluding deoxyribonucleic acid (DNA) analysis from conventional sites. However, most of the teeth survived the conflagration. They were used to identify the victim. Additionally, the dental pulps were found to be an excellent source of high molecular weight genomic DNA. This proved to be an effective method to link the victim's body to biological evidence recovered from the site of the murder.

Adolescent psychopathy in relation to delinquent behaviors, conduct disorder, and personality disorders.

Abstract: The purpose of this study was to explore the relationship between psychopathy as measured by The Revised Psychopathy Checklist (PCL-R) and delinquent behaviors, conduct disorder, and personality disorders in psychiatrically hospitalized adolescents. Thirty adolescent inpatients were assessed for psychopathy, delinquent behaviors, DSM-III-R Axis I disorders, and personality disorders using the Revised Psychopathy Checklist (PCL-R), the Diagnostic Interview for Children and Adolescents (DICA-R), and the Structured Interview for DSM-III-R Personality Disorders (SIDP-R). Significant relationships were noted between elevated PCL-R psychopathy scores and delinquent behaviors, conduct disorder, and narcissistic personality disorder. The validity of the PCL-R as a measure of psychopathy in adolescence was supported. Longitudinal studies are needed to clarify the clinical application of the PCL-R to adolescent populations.

D1S80 population data in African Americans, Caucasians, southeastern Hispanics, southwestern Hispanics, and Orientals

Abstract: Allele frequencies for the locus D1S80 were determined in African American, Caucasian, Southeastern Hispanic, Southwestern Hispanic, and Oriental sample populations using the polymerase chain reaction and subsequent electrophoresis and silver staining of the amplified products. Due to the presence of anodal and cathodal electrophoretic variants (in reference to the steps in an allelic ladder), allele frequencies were established using a classification protocol based on the steps in the allelic ladder. All sample populations met Hardy-Weinberg expectations for D1S80. In addition, there was no evidence for association of alleles between the loci D1S80 and D1S7. The product of allele frequencies from the data from the sample populations in this study can be used in forensic analyses and paternity tests to estimate the frequency of a D1S80 DNA genotype.

Techniques for Locating Burials, with Emphasis on the Probe

Abstract: The search for buried remains frequently includes visual assessment of surface features, trained dogs, and sophisticated geophysical remote sensing techniques. Nonintrusive, electronic survey equipment, such as the proton magnetometer, ground penetrating radar, and electrical resistivity, have yielded good results. However, under certain field conditions a simple, less expensive, relatively noninvasive tool—the probe—is effective. The probe, when used by an experienced investigator, provides a variety of information in a short amount of time, facilitates excavation, and minimizes damage to a burial. This paper offers examples of the application of a probe in forensic cases in urban and rural settings and in the detection of historic burials. Examples include the location of four individuals killed during the raid on the Branch Davidian Compound in Mount Carmel, Texas, and the search for burials in cemeteries that had been desecrated.

Testing human hair for cannabis. II. Identification of THC-COOH by GC-MS-NCI as a unique proof.

Abstract: To validate information on cannabis use, 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic (THC-COOH) was investigated in human hair. The identification of THC-COOH in hair would document cannabis use more effectively than the detection of the parent drug which might have come from environmental exposure in a smoky atmosphere. Samples (100 mg) were decontaminated with methylene chloride and destroyed by incubation in 1 mL of 1 N sodium hydroxide for 30 min at 95 degrees C in presence of 10 ng of THC-COOH-d3. After cooling, samples were extracted by n-hexane/ethyl acetate after acidification with acetic acid. After derivatization by PFPA-PFP-OH of the dry extract, drugs were separated on a HP1 capillary column, and detected by mass spectrometry (m/z 602) using negative chemical ionization with methane as reagent gas. Among 30 samples obtained from subjects deceased from fatal heroin overdose, 17 tested positive for THC-COOH, in the range 0.02-0.39 ng/mg, with an average of 0.12 ng/mg.

Myocardial hypertrophy and coronary artery disease in male cocaine users.

Abstract: We compared the heart weights and the incidence of atherosclerotic lesions in trauma fatalities testing positive for cocaine with the hearts of cocaine negative trauma fatalities, with the hearts of decedents dying from cocaine overdose, and with historic controls derived from the literature. There were 116 trauma victims, 83 men and 33 women, aged 20–50 years, and 26 men with cause of death listed as cocaine toxicity. The 32 cocaine (+) male trauma fatalities had a mean age 34 ± 10 years and a mean heart weight of 375 ± 82 grams. The 51 cocaine free males had a mean age 31 ± 9 years, and mean heart weight 337 ± 54 grams (P = .01). Heart weights in the cocaine using group were also significantly greater than historical controls from World War I, Korea, and Vietnam. The incidence of atherosclerotic changes was similar in both groups, but cocaine (+) men had more frequent coronary artery lesions involving multiple vessels (P = .01). Comparisons between 16 cocaine (+) females and 17 cocaine (−) female controls disclosed no significant differences in heart weight or the frequency of atherosclerotic lesions. The 26 men dying of cocaine overdose also had larger hearts than the controls (379 ± 64 g, P = .004), and more severe CAD (30% had involvement of 2 or more vessels, P = .02). The degree of myocardial hypertrophy documented in this study was highly significant, but because the increase is modest (around 10%), it is likely to go unrecognized at autopsy. Cardiac enlargement and coronary artery disease in cocaine users may be much more prevalent than has previously been appreciated, and may be responsible for some unexplained cases of sudden death in cocaine users.

Signature Murders: A Report of Several Related Cases

Abstract: Three female patrons of singles bars were murdered in separate events in Bellevue, Washington in 1990 within sixty-seven days of each other. An evaluation of those murders revealed that all the murders were linked by a distinct personal "signature" or "calling card" left by the killer. As reported in the literature, the modus operandi of a killer frequently changes from one murder to the next and is different from the killer's signature, which is a permanent psychological imprint at each scene. The murder cases reported here demonstrate an elaborate signature of one killer who engaged in a rare personalization of his necrophilic fantasies by posing, openly displaying, and sexually inserting foreign objects into each one of three victims' bodies.

Further comments on the estimation of error associated with the Gustafson dental age estimation method.

Abstract: Many researchers in the field of forensic odontology have questioned the error estimates stated in Gustafson's paper outlining the relationship between certain dental attributes and age. In a substantial re-working of Gustafson's data, Maples and Rice corrected Gustafson's regression statistics and found that the error associated with the age estimate was nearly double that claimed by Gustafson. We offer another statistical analysis of Gustafson's data and find that the errors calculated by Maples and Rice were also in error, being about a year too small. We give a formula for what we believe to be the correct treatment of errors in such cases, but conclude by observing that there is an urgent need for a more rigorous study of the traits first tabulated by Gustafson.

The role of ethanol in heroin deaths.

Abstract: The purpose of this study was to evaluate the role of ethanol in deaths due to heroin intoxication. Over a 12 month period, all cases investigated by the Office of the Chief Medical Examiner, State of Maryland where a blood screen by Roche Abuscreen radioimmunoassay (RIA) was positive at a cutoff of 100 ng/mL were included in the study. Free morphine was quantitated using the Coat-A-Count RIA and ethanol was quantitated by head space gas chromatography. All presumptive morphine positive cases were confirmed by gas chromatography/mass spectrometry. Seventy of the 119 cases where death was attributed to narcotic or alcohol and narcotic intoxication had blood ethanol concentrations (BAC) greater than or equal to 0.02 g/dL; 48 had BAC ≥ 0.10 g/dL. Only 3 of 45 cases where morphine was identified but was unrelated to death had BAC ≥ 0.02 g/dL. At all ranges of free morphine concentrations, there was a greater percentage of narcotic deaths when ethanol was present. From the data, we conclude that 1) the use of even small amounts of ethanol with heroin is clearly a risk factor in deaths due to heroin, 2) there are some heroin deaths where no free morphine is identified in the blood. In these deaths, ethanol is unlikely to be present, 3) at blood ethanol concentrations between 0.20 and 0.29 g/dL, the morphine concentrations in heroin deaths increased significantly, 4) at blood ethanol concentrations greater than 0.30 g/dL, morphine became less of a factor than the ethanol in causing death.

Qualitative and Quantitative Determination of Residual Solvents in Illicit Cocaine HCl and Heroin HCl

Abstract: Methodology has been developed which utilizes static headspace-gas chromatography-mass spectrometry (SHS-GC-MS) to identify and quantitate residual solvents occluded in illicit cocaine HCl and heroin HCl. The liberation of the occluded solvents was ensured by complete solubilization of the crystal matrices in aqueous 22% sodium sulfate. Ion trap mass spectrometry is used for both identification and quantitation; five deuterated, structurally related internal standards are utilized for more accurate quantitation. Overall method precision for 25 commonly encountered solvents averaged 6.7% RSD. Minimum detection limits ranged from 3 to 87 ppm for a 15 mg equivalent cocaine sample weight, and from 2 to 43 ppm for a 30 mg equivalent heroin sample weight. Qualitative and quantitative data for the 25 most commonly encountered occluded solvents in cocaine HCl and heroin HCl exhibits are presented.

Diameter of cranial gunshot wounds as a function of bullet caliber.

Abstract: Determination of bullet caliber becomes increasingly important in homicides where the bullet is missing. In cases with entrance wounds to bone that are circular and well defined it may be tempting to measure the defect and offer suggestions about bullet caliber. For this reason, the relationship between wound diameter and bullet caliber was examined using cranial bones from autopsy cases. The minimum diameter of 35 cranial wounds produced by .22, .25, and .38-caliber bullet was measured. The relationship of minimum wound diameter to bullet caliber was examined using a one way analysis of variance. Fisher's least significant difference test revealed no significant difference between .22-caliber and .25-caliber wounds, while the .38-caliber wounds were significantly different (P