Showing papers in "Journal of Lipid Research in 1971"

TL;DR: The quantitative isolation of total glycosphingolipids from crude lipid extracts without contamination from other lipid classes is described and is useful for determination of microgram quantities of glycolipids derived from less than 1 ml of packed cells.

TL;DR: In the majority of cases the accumulation of cholesterol in body pools was small because of adequate compensation by reexcretion plus reduced synthesis, but in a few patients large accumulations occurred on high cholesterol diets when absorption exceeded the compensatory mechanisms.

TL;DR: A method for calculating mean fat cell weight using osmium-fixed fat cells is described, which makes determinations of sample wet weight and ratio of lipid to wet weight unnecessary.

TL;DR: A new metabolite of vitamin D(3) has been demonstrated in the plasma of rats and in the intestines of chicks given 100 IU of vitaminD(3)-1,2-(3)H.

TL;DR: After oral administration of lithocholate-(14)C to three patients with cholelithiasis, radioactive metabolites having the chromatographic properties of sulfated lithocholates were isolated from bile and were identified as sulfated glycolithocholate and taurolitholates by their characteristic chromatography mobilities during a series of specific hydrolytic procedures and by crystallizing them to constant specific activities with the synthetic sulfates.

TL;DR: The enlarged adipose depots of the adult Zucker "fatty," when compared with the nonobese control, are the result of both hypertrophy and hyperplasia.

TL;DR: Astrocytes have a higher ganglioside content than neuronal perikarya, a finding ascribed to the higher ratio of surface membrane to mass in the astrocytes, and considered as evidence thatgangliosides are normal glial constituents.

TL;DR: Two improvements are described for the assay of HMG CoA reductase, including a simple synthesis of the substrate precursor HMG-3-(14)C anhydride and a double-label method for determining the amount of mevalonate-3-C that is formed from the substrate.

TL;DR: Free fatty acid release from fat cells, obtained from epididymal adipose tissue of rats of different sizes, was found to be dependent on the cell surface area, regardless of the age of the animals.

TL;DR: The results show that the larger cells of a specimen have a greater rate of lipid synthesis than the smaller cells of the same specimen, mainly due to an increase in the synthesis of glyceride-glycerol.

TL;DR: Although Method IV requires collections of stools for up to 8 days, it is nevertheless the most rapid and the simplest of all the methods for estimating absorption and would seem to be a valuable addition to other isotopic techniques for estimating cholesterol absorption in man.

TL;DR: The effect of feeding a semipurified diet high in sucrose on serum lipid and lipoprotein concentrations was studied, and VLDL showing the most marked effect showed depressed incorporation of labeled amino acids into lipoproteins and other plasma proteins.

TL;DR: A new mechanism for the subterminal oxidation of methyl ketones by microorganisms is proposed whereby the first intermediate produced is an acetate ester which subsequently is cleaved to acetate and a primary alcohol two carbons shorter than the original ketone substrate.

TL;DR: Sulfate esterification has been shown previously to be a prominent feature of lithocholate metabolism in man, and these studies were undertaken to ascertain whether this metabolic pathway is also present in rats, and to investigate the physiological significance of bile acid sulfate formation.

TL;DR: The results suggest that acyl transferases may be important in the lung to insure that sufficient amounts of dipalmitoyl phosphatidylcholine will always be present for use in pulmonary surfactant biosynthesis.

TL;DR: Ceramides with mono-, di-, and trihydroxy long-chain bases, and normal (saturated and unsaturated), branched-chain, and 2-hydroxy fatty acids have been analyzed by thin-layer chromatography.

TL;DR: The present data are consistent with an active synthesis of the monoenoic and dienoic phosphatidylinositols by way of the phosphatidate, followed by a deacylation-reacylation cycle involving arachidonic acid, as claimed for other rat liver glycerophosphatides.

TL;DR: It was demonstrated that norepinephrine-activated adenyl cyclase activity expressed per 10(6) cells was unaffected by cell size, leading to the suggestion that the number of adrenergic receptors in the fat cell is fixed and is independent of the size of the cell; as the cell enlarges, these receptors are merely distributed over a greater surface area.

TL;DR: The overall results indicate that a direct exchange between free choline and the choline moiety of phos- phatidylcholine does not play a significant role in the incorpora- tion of choline into phosphatidycholine by Novikoff cells or in the turnover of the ch Caroline moiety in these cells, and that labeled choline therefore is a useful precursor in studying the synthesis and turnover of membrane phosphati- dyl choline in these Cells.

TL;DR: It is suggested that myelin in the quaking mouse has certain compositional similarities with juvenile myelin, but it may be an abnormal type of myelin.

TL;DR: Electro microscopic studies of rat and human intestinal mucosa provide further evidence for the production of VLDL in absorptive cells of fasting rats and human intestine, and support the concept that the small intestine is a source of endogenous plasma V LDL.

TL;DR: The results show that tomatine has an effect on cholesterol absorption and on other aspects of lipid metabolism in the rat similar to that of cholestyramine, with the notable exception that tomato increased sterol excretion while cholencyramine increased bile acid excretion.

TL;DR: High carbohydrate diets enhance the hepatic output of very low density lipoprotein triglycerides in rats by enhanced formation of VLDL triglyceride from exogenous free fatty acids, and carbohydrate feeding did not result in altered uptakes of free fatty fatty acids or preferential secretion of triglycerides containing endogenously synthesized fatty acid.

TL;DR: In vitro enzyme studies indicate that the increase in phosphatidylcholine content of phenobarbital-induced proliferating endoplasmic reticulum is related to increased activity of the pathway of phosphatidocholine biosynthesis involving the sequential methylation ofosphatidylethanolamine.

TL;DR: Long-term studies at 80 degrees C demonstrated that BCl(3)-CH(3)OH caused less loss of unsaturated acid than did BF(3-CH( 3)OH, and it was demonstrated that boron trihalide-methanol produced esters apparently free from contaminants.

TL;DR: The results indicate that liver liposomes from orotic acid-fed rats apparently contain the low density apoprotein and probably several other very low density lipoprotein peptides.

TL;DR: It has been concluded that reliable structural information can be obtained from small amounts (less than 50 micro g) of a purified glycosphingolipid.

TL;DR: The results suggest that the insensitivity of cholesterol biosynthesis to dietary cholesterol in hepatomas could be explained by an impairment in the uptake and storage of dietary cholesterol and that the concept of an intracellular deletion of the feedback mechanism requires further evidence.

TL;DR: Glucosylceramide was the only glycolipid found in seven out of eight analyzed samples of lymphocytes, both normal and leukemic, in preparations consisting mainly of polymorphonuclear, myeloid, and blastic cells.

TL;DR: Evidence is presented to support the conclusions that the 95,000 g-supernatant fraction of rabbit lung contains the enzymes necessary for de novo fatty acid synthesis and is capable of synthesizing long-chain fatty acids, probably palmitic acid, under the appropriate conditions.