Showing papers in "Journal of Neuroscience Methods in 1986"

TL;DR: The usefulness of a number of new stereological principles for unbiased estimation of particle number and sizes and sampling of particles is illustrated together with a novel principle for biased estimation of anisotropic surfaces.

TL;DR: The procedure liberates neurons free of cellular debris and glial investments, allowing the formation of giga-ohm seals with patch clamp electrodes, and the characteristic morphology of neurons is maintained.

TL;DR: Fluorescein- and rhodamine-conjugated dextran-amines are used as axonal tracers in in vitro preparations of the nervous system of the chicken embryo to reveal the dendritic and axonal structure of labelled neurons.

TL;DR: Metric analysis methods used to study neuronal arborizations are reviewed and the instrumentation used for providing 3-dimensional data for metric analyses and the shrinkage of Golgi-stained neurons are discussed.

TL;DR: Comparison of the different approximation equations and procedures to estimate the volume of a brain region with an irregular shape contained in parallel sections indicates that the 'basic volume estimator' using systematic section is very efficient and sufficiently accurate.

TL;DR: The peristimulus time histogram (psth) provides a means of correlating the discharges of neurones with other events and the theory of stochastic point processes is used to derive an algorithm for calculating the best approximation of variance of the cusum.

TL;DR: In a newly developed stereotaxic system experimentally measured patterns of skull profile lines are used to define the animal's brain position with an accuracy of +/- 100 microns, which facilitates the compilation and comparison of data within and among animals.

TL;DR: A dynamic model of the jaw system of the rat has been formulated, relating force with fiber length based on sarcomere morphometric information and the static skeletal muscle model.

TL;DR: An increasing accuracy of results regarding density and amount of neurons can be estimated with facilitated counting procedures and a similar development can be observed for the estimation of neuronal sizes and their size distribution as well as for the volume-parts of the average perikaryon volume fraction (grey cell coefficient).

TL;DR: A data set consisting of the locations of "light-on" and " light-off" displaced amacrine cells in the retina of a rabbit is analysed using recently developed statistical methodology to discriminate between two biological hypotheses concerning the genesis of the data.

TL;DR: From the results it is concluded that it is not advisable to use an unfolding technique to estimate the number and size of synapses since all sorts of assumptions regarding the shape, truncation and overprojection are hazardous.

TL;DR: Catecholamine release from perfused cat adrenal glands has been continuously monitored by on-line connection of the perfusion fluid emanating from the gland to an electrochemical detector, avoiding the time-consuming procedures of collecting samples and assaying them individually through tedious fluorimetric or radioenzymatic techniques.

TL;DR: Laminin immunohistochemistry has proven to be a simple, useful and specific method to study vascular structures in the central nervous system and an excellent alternative to more conventional and laborious methods such as perfusion with India ink.

TL;DR: A computerized image processing system for quantitative receptor autoradiography on tritium-sensitive film and the calibration of the system which establishes the non-linear relation between grey values and concentration of radioactivity are discussed.

TL;DR: The performance of SPIFODIS was compared quantitatively with that of a conventional amplitude trigger in two cases: when detecting a single unit with varied signal-to-noise ratios and when separating double units of equal amplitude.

TL;DR: Even cells exhibiting poor electrophysiological characteristics, or cells impaled only briefly, frequently exhibited good quality dye filling, and this method will be particularly useful when large sample sizes are necessary to compare regional variations in the extent of electrotonic coupling in the mammalian brain.

TL;DR: The underlying assumptions and statistics of the method most frequently used: ordinary linear regression, principal axis and standard major axis are focused on.

TL;DR: The intention is to present quick and easy methods that are applicable to camera lucida drawings that can be used for the identification and the study of the changes in the nervous system during development or as a result of disease or an experimental treatment.

TL;DR: A video digitizing system designed for measuring film optical density, which is based on a 6-bit (64 gray level) digitizer, solid state video camera, and Apple II microcomputer, digitizes a rectangular area selected by the operator and converts the gray level values into preselected standard units.

TL;DR: A computerized processing system is developed that digitizes EMG activity and calculates auto- and cross-correlation products of selected segments that provide a general, quantitative tool for the study of an important aspect of motor control that may be overlooked by conventional sampling and smoothing techniques.

TL;DR: In this study, the grey level index (GLI) is measured with a TV-based image analyzer from routine histological sections, which is a biased estimate of the volume density of Nissl-positive structures.

TL;DR: The method generates reliable and reproducible quantitative values from very small brain regions and nuclei, combining the quantitative character of a radiometric assay with the superior anatomical resolution of histochemistry.

TL;DR: Consistent, complete lesions restricted to the appropriate sensory and motor neurons are documented after injection of the vagus, hypoglossal, phrenic and sciatic nerves and the superior cervical ganglion.

TL;DR: WGA-HRP and CTB were better than HRP and twice as effective as CTB- HRP, and Fast blue and HRP were equally effective.

TL;DR: A miniature cryoprobe for blockade of neuronal structures using Freon as a cooling medium and consists of concentric double stainless steel cannulae, a shaft heater coil and a tip thermocouple is developed.

TL;DR: A simplified method is described for the isocratic analysis of endogenous amino acid neurotransmitters contained in brain perfusates by high performance liquid chromatography (HPLC) with electrochemical detection (EC).

TL;DR: The usefulness of the perfusion-dialysis method is demonstrated for studies of, for example chemically evoked changes in Ca2+ concentrations extracellularly in CNS regions of non-anesthetized, freely moving rabbits, as it is easily correlated with neurotransmitter levels in the same samples.

TL;DR: The most widely used conventional stereotactic method utilizing the Horsley-Clarke coordinate system does not allow accurate intracerebral placements, so the technique based on ventricular landmarks utilizing "orthogonal teleradiography" can be resolved.