Showing papers in "Journal of Virological Methods in 2022"

TL;DR: Wang et al. as mentioned in this paper evaluated the test accuracy of reverse-transcription loop-mediated isothermal amplification (RT-LAMP) and reverse transcription-PCR for the diagnosis of coronavirus disease 2019 (COVID-19).

TL;DR: In this article , the authors evaluated Allplex SARS-CoV-2 Master Assay and Variants I Assay to detect HV69/70 deletion, Y144 deletion, E484K, N501Y, and P681H spike mutations in 248 positive samples collected in Kuala Lumpur, Malaysia, between January and May 2021.

TL;DR: In this article , the authors used the Autoregressive Integrated Moving Average (ARIMA) model to analyze the temporal dynamics of the worldwide spread of COVID-19 in the time window from January 22, 2020 to April 7, 2020.

TL;DR: In this paper , the authors investigated the basic growth parameters of HCoV-OC43 infection in three cell lines (HRT-18, human lung fibroblasts (MRC-5) and African green monkey kidney (Vero E6) including the differential development of cytopathic effect (CPE) and explored reducing the cost, time and complexity of antibody-based detection assay.

TL;DR: In this paper, two ELISA-based SARS-CoV-2 surrogate neutralization assays (sVNTs) were compared for detecting humoral neutralizing activity in individuals vaccinated with the spike-based vaccine mRNA-1273.

TL;DR: In this article, the authors investigated the basic growth parameters of HCoV-OC43 infection in three cell lines (HRT-18, human lung fibroblasts (MRC-5) and African green monkey kidney (Vero E6) including the differential development of cytopathic effect (CPE) and explored reducing the cost, time and complexity of antibody-based detection assay.

TL;DR: In this paper , two ELISA-based SARS-CoV-2 surrogate neutralization assays (sVNTs) were compared for detecting humoral neutralizing activity in individuals vaccinated with the spike-based vaccine mRNA-1273.

TL;DR: Wang et al. as mentioned in this paper developed a loop-mediated isothermal amplification (LAMP) for the visual detection of porcine circovirus 4 and evaluated its sensitivity, specificity, and applicability in clinical samples.

TL;DR: In this paper , an easy, cheap and fast method for the detection of mutations in the spike protein that are indicative for specific variants of SARS-CoV-2 has been described.

TL;DR: Wang et al. as mentioned in this paper developed a loop-mediated isothermal amplification (LAMP) the assay for the visual detection of PCV4 and evaluated its sensitivity, specificity, and applicability in clinical samples.

TL;DR: In this article , the authors highlight the possible entry molecules, pathogenesis, symptomatology, probable cure and the recently developed vaccines for the existing pandemic due to the COVID-19.

TL;DR: In this paper , a real-time reverse transcription PCR (rRT-PCR) was used to identify H5Nx HPAIV RNA and H5 subtyping using Sanger sequencing of the heamagglutinin (HA) cleavage site.

TL;DR: In this paper , the Salocor SARS-CoV-2 N-antigenemia was used as a diagnostic marker in acute COVID-19 patients and the sensitivity of the SARS N antigen test was 95.6 % within 14 days post onset of symptoms.

TL;DR: In this article, the authors developed an in-house TaqMan minor groove binder probe-based one-step RT-qPCR assay to detect the presence of N501Y mutation in SARS-CoV-2.

TL;DR: In this paper , the authors developed an in-house TaqMan minor groove binder probe-based one-step RT-qPCR assay to detect the presence of N501Y mutation in SARS-CoV-2.

TL;DR: In this paper , an isothermal-based one-step reverse transcription-recombinase polymerase amplification (RT-RPA) approach was developed to detect PVS infection in potato leaves and tubers.

TL;DR: In this paper , the authors performed a systematic review on the SARS-CoV-2 pneumonia and found that sensitivity and specificity were 0.97 (0.96 to 0.98) and 0.03 (0.,01 to 0.,10) respectively, and AUC was 0.9926.

TL;DR: In this paper , a double antigen binding assay (DABA) was used to detect anti-RBD in human and ferret immunised with ChadOx1 nCoV-19 vaccine and human immunized with both AstraZeneca and Pfizer vaccines.

TL;DR: In this paper , two automated SARS-COV-2 anti-S1 RBD immunoglobulin serology assays on the Atellica IM Analyzer were calibrated to WHO 20/136 standard reference material which was assigned 1000 binding antibody units (BAU/mL).

TL;DR: In this paper , the effect of primer mismatches on RT-qPCR performance in vitro using synthetic mismatch in vitro transcripts was investigated and it was shown that a mismatch in the forward primer has a more detrimental effect for PCR performance than a mismatching in the reverse primer.

TL;DR: In this article , an enhanced rolling circle amplification (RCA) method using EquiPhi29 polymerase was combined with size selection to generate a cost-effective, short-read sequencing method.

TL;DR: In this paper , the authors reviewed COVID-19 diagnostic assays for clinical application, including nucleic acid tests, immunological methods, and computed tomography (CT) imaging.

TL;DR: In this paper , the authors investigated whether the reduced amplification efficiency denoted by an increased RT-PCR cycle threshold value (RΔE) can be used as an indirect measure of SARS-CoV-2 Delta variant prevalence.

TL;DR: In this article, the effect of primer mismatches on RT-qPCR performance in vitro using synthetic mismatch in vitro transcripts was investigated and it was shown that a mismatch in the forward primer has a more detrimental effect for PCR performance than a mismatching in the reverse primer.

TL;DR: SpikeSeq as discussed by the authors is a Sanger sequencing-based method for the detection of Variants of Concern (VOC) and key mutations, using a 1 kb amplicon from the recognized ARTIC Network primers.

TL;DR: In this article , the authors evaluated the performance of a rapid immunochromatographic test for the detection of SARS-CoV-2 antigen, in comparison to Reverse transcription polymerase chain reaction (RT-PCR).

TL;DR: In this article , the authors summarized the currently available direct and indirect testing platforms in research and clinical settings, including recent progress in the methods to detect viral RNA, antigens, and specific antibodies.

TL;DR: In emergency room, point-of-care antigen testing for SARS-CoV-2 antigen can expedite clinical strategies for patient management as discussed by the authors, which is highly valuable.

TL;DR: In this paper , a duplex TaqMan real-time quantitative reverse transcription PCR (RT-qPCR) assay was developed to distinguish the two genotypes of Goovirus (GAstV) and GAstV-2.

TL;DR: In emergency room, point-of-care antigen testing for SARS-CoV-2 antigen can expedite clinical strategies for patient management as discussed by the authors , which is highly valuable.