Showing papers in "Laboratory Investigation in 2022"

TL;DR: The 5th edition of the World Health Organization Classification of Tumors of the Central Nervous System adopted new molecular markers into the revised grading criteria of IDH-mutant and -wild-type astrocytoma as mentioned in this paper .

TL;DR: In this article , the authors used the AQUA method of quantitative immunofluorescence to test a range of antibody concentrations to maximize the sensitivity within the lower range of HER2 expression.

TL;DR: In this article , the authors used the AQUA method of quantitative immunofluorescence to test a range of antibody concentrations to maximize the sensitivity within the lower range of HER2 expression.

TL;DR: In this paper , the availability of specific and reliable assays for the SARS-CoV-2 viral genome and its proteins is essential to identify the infection in suspected populations, make diagnoses in symptomatic or asymptomatic individuals, and determine clearance of the virus after the infection.

TL;DR: Proteomics plays a vital role in biomedical research in the post-genomic era as discussed by the authors , and the advances in high-throughput proteomics will not only help better understand the molecular mechanisms of pathogenesis, but also to identify the signature signaling networks of specific diseases.

TL;DR: In this paper , the authors examined suggested mechanisms of COVID-19-associated heart failure in 21 positive decedents, obtained through standard autopsy procedure, compared to clinically matched controls and patients with various etiologies of viral myocarditis.

TL;DR: In this paper , the role of circST6GALNAC6 in ferroptosis was investigated in the presence of erastin in the N-terminus of small heat shock protein 1 (HSPB1).

TL;DR: In this paper , the authors explored the biological role of long non-coding RNA (lncRNA) MAPKAPK5_AS1 (MAAS) and the mechanism of its differential expression in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC).

TL;DR: In this article , the role of circST6GALNAC6 in ferroptosis was investigated in the presence of erastin in the N-terminus of small heat shock protein 1 (HSPB1).

TL;DR: Wang et al. as mentioned in this paper presented a deep learning system to achieve interpretable tumor differentiation grade and microsatellite instability (MSI) recognition in gastric cancer directly using HE staining whole-slide images (WSIs).

TL;DR: This article showed that MMP11 expression in macrophages is an independent negative prognostic factor in breast cancer, whereas MMP 11 expression in tumor cells and fibroblasts is not associated with patient survival.

TL;DR: In this paper , the authors examined 13 MMRD-associated primary brain tumors to determine molecular characteristics and biological behavior, and found that the most common MMRD primary brain tumor was GBM IDH-wildtype.

TL;DR: In this paper , qRT-PCR and western blot assays were used to measure the expression of genes and proteins, and they indicated an oncogenic role of circN4BP2L2 in CRC via regulation of the miR-340-5p/CXCR4 axis.

TL;DR: In this article , the therapeutic potential of HOTAIRM1 transferred by alveolar epithelial cell (AEC)-derived exosomes in interstitial pulmonary fibrosis (IPF) and the potential molecular mechanisms were investigated.

TL;DR: In this article , in-vitro, human cardiac myocytes (HCMs) were treated with hypoxia/reoxygenation (H/R), and the expression of miR-185-5p and ROR, IL-1β and IL-18 were detected by qRT-PCR.

TL;DR: The authors developed a concise and efficient survival analysis model, named CNN-Cox, which combines a special CNN framework with prognosis-related feature selection cascaded Wx, with the advantage of less computation demand utilizing light training parameters.

TL;DR: In this paper , the role and underlying molecular mechanisms of USP35 in chemoresistance of non-small cell lung cancer (NSCLC) to cisplatin were investigated.

TL;DR: In this paper , the therapeutic potential of HOTAIRM1 transferred by alveolar epithelial cell (AEC)-derived exosomes in interstitial pulmonary fibrosis (IPF) and the potential molecular mechanisms were investigated.

TL;DR: Wang et al. as mentioned in this paper investigated the possible effect of bioactive glass (BG) on pyroptosis in wound healing both in vivo and in vitro, and showed that BG can accelerate wound closure, granulation formation, collagen deposition, and angiogenesis.

TL;DR: In this article , the authors conducted a validation study, assessing concordance across serial sections of a tissue microarray that were independently imaged by MIBI-TOF or single-plex immunohistochemistry (IHC) over 12 days.

TL;DR: In this paper , the authors found that fatty acid binding protein 4 (FABP4) plays an important role in macrophage inflammation and lipid metabolism in atherosclerosis induced by Hcy.

TL;DR: In this paper , the effects of Nrf2 downregulation on radiosensitivity and the involvement of glycolysis in nrf2-mediated radioresistance in oral squamous cell carcinoma (OSCC) were evaluated.

TL;DR: Wang et al. as discussed by the authors analyzed transcriptional regulation of the PD-L1 promoter by HIF2α and IFNγ-inducible interferon regulatory factor-1 (IRF-1) in ccRCC cells.

TL;DR: In this paper , the authors defined the evolution of macrophage polarization in acute and chronic gout in rat gout and found that the number of M1 macrophages was comparable to that of M2 from 2 to 12 h and exceeded M2 number at 18 h and 24 h.

TL;DR: Zhang et al. as discussed by the authors showed that higher PLCG1 expression was associated with tumor growth and worse prognosis in lower-grade glioblastoma (LGG) patients.

TL;DR: In this article , the hepatocyte-specific enhancer/promoter of FGF21 expression plasmids in high-fat diet-fed mice for 12 weeks was examined. And the results showed that FGF-21-induced lipolysis in the adipose tissue enabled the liver to be flooded with fat-derived FFAs.

TL;DR: In this paper , the authors used CRISPR-Cas9 and somatic cell cloning to produce pigs with the unique mutation c.152_153insG (PKD1insG/+).

TL;DR: Wang et al. as discussed by the authors found that LINC01963 was overexpressed in the PC3-DR cells and inhibited the chemoresistance of PC3 cells to docetaxel.

TL;DR: In this paper , the role of miR-200c in cholestasis-induced biliary liver fibrosis and cholangiocyte activation was investigated in a bile duct ligation (BDL) mouse model.

TL;DR: In this paper , the authors showed that exosome-derived noncoding RNAs (ncRNAs) are highly involved in the progression of acute and chronic liver diseases by regulating hepatic lipid metabolism, innate immunity, viral infection, fibrosis, and cancer.