Sandra Martinez-Morilla

TL;DR: The reproducibility of Ki67 measurement between three image analysis platforms with supervised classifiers performed by the same operator, by multiple operators, and finally in prognostic potential is investigated to justify multi-institutional DIA studies to assess clinical utility.

TL;DR: Terminal erythroid maturation involves migration of histones into the cytoplasm via a process likely facilitated by Xpo7, and many DNA binding proteins such as histones H2A and H3 were found to have migrated into the Cyprolasm of normal late erythroblasts prior to and during enucleation, but not in XPO7-knockdown cells.

TL;DR: Commercial use of this Index TMA or similar smaller version is envisioned as a useful standardization mechanism to compare results between institutions and to identify abnormalities while running routine clinical samples.

TL;DR: In this article , the authors used the AQUA method of quantitative immunofluorescence to test a range of antibody concentrations to maximize the sensitivity within the lower range of HER2 expression.

TL;DR: A protocol that is used to validate antibodies specifically for immunohistochemistry is described, including some of the pillars of antibody validation from Uhlen et al. 2016, as an example of a rigorous approach to build antibody-based tests for both basic and translational science labs and for the clinic.