Showing papers in "Natural Product Research and Development in 2011"

Composition and Antimicrobial Activities of Essential Oil of Fructus Amomi

Abstract: The essential oil extracts were respectively obtained from the seeds and husks of Amomum villosum Lour by using Bligh-Dyer methods for the first timeOne hundred and thirty-eight compositions were identified by GC-MS from four essential oil extractsThe main components of the essential oil were bornyl acetate,camphor,borneol,α-pinene,β-pinene and α-copalene,etcThe relative contents of total alkenes and ketones composition accounted for 10%-40% in integral of essential oilThirty-five compositions such as neophytadiene,globulol,bicyclogermacrene,etc were identified from this plant firstlyThe results of inhibitory effect for bacteria and fungi showed that the essential oil had remarkable inhibitory effects for Trichophyton rubrum,Trichophyton mentagrophyton,Microsporum gypseum,Staphyloccocus aureus,and Enterococcus faecalisTherefore,the essential oil can be used in the food industry as raw food material or natural food preservation

Flavonoids of Rhizoma Sparaganii Induce S/G_2 Stage Arrest in A549 and MCF-7 Cells

Abstract: To investigate the cytotoxicity effects and mechanisms on estrogen receptor positive(ER+) cancer cell lines A549 and MCF-7 of the Rhizoma Sparganii flavonoids(RSF),A549 or MCF-7 cells in DMEM mediums with 188,375,and 750 μg/mL RSF were cultured for 3 days,respectively.DMEM with 1% methanol culture cells as control.The cell proliferation activity,anti-a-tublin form,apoptosis as well as cell cycle differences between groups were studied by MTT,immunofluorescence,and flow cytometry technology.As a result,compare to the 1% methanol control groups,RSF could inhibit the proliferation activity,increase the rate of apoptotic bodies,and induce cytoskeleton disorder of the A549 and MCF-7 cells with the dose-related.In addition,S/G2 stage arrests were induced obviously in A549 and MCF-7 cells cultured with 375 and 750 μg/mL RSF.The results indicated that RSF showed a notable cytotoxicity against A549 and MCF-7 cells,and the inhibitions of cell proliferation were related with S/G2 cell cycle arrests.

Chemical Constituents from Acorus tatarinowii

Abstract: Eighteen compounds were isolated from the rhizome of Acorus tatarinowii.Their structures were identified by spectroscopic data as(7S,8R)-4,9′-dihydroxyl-3,3′-dimethoxyl-7,8-dihydrobenzofuran-1′-propylneolignan(1),(7S,8R)-4,9′-dihydroxyl-3,3′-dimethoxyl-7,8-dihydrobenzofuran-1′-propylneoligan-9-O-β-D-glucopyranoside(2),7′-hydroxylariciresinol-9-acetate(3),5-hydroxy-3,7,4′-trimethoxyflavone(4),rhoifolin(5),astragalin(6),pinocembrin-7-O-rutinoside(7),kaempferol-3-O-rutinoside(8),rhodionin(9),isoschaftoside(10),5-hydroxymethyl-2-furaldehyde(11),trans-cinnamic acid(12),3,7-dihydroxy-11,15,23-trioxo-lanost-8,16-dien-26-oicacid(13),3,7-dihydroxy-11,15,23-trioxo-lanost-8,16-dien-26-oic acid methyl ester(14),cycloartenol(15),daucosterol(16),lupeol(17),(22E,24R)-ergosta-5,7,22-trien-3β-ol(18).Except for compounds 4,11,and 16,all the other substances were obtained from this plant for the first time.

Chemical Constituents of Meconopsis punicea

Abstract: Seventeen compounds were isolated from the aerial parts of Meconopsis punicea.On the basis of MS and NMR data,their structures were identified as karachine(1),valachine(2),dihydrosanguinarine(3),(–)-mecambridine(4),protopine(5),oleracein E(6),anhydroberberillic acid (7),berberine(8),alborine(9),luteolin(10),tricin(11),dihydroquercetin(12),apigenin(13),hydnocarpin(14),tricin 7-O-β-D-glucopyranoside(15),p-coumaric acid(16) and uracil(17).Among them,compound 1,2,3,7,15 and 17 were isolated from the genus for the first time,and compound 6,8,11 and 14 were isolated from the species for the first time.2D NMR techniques including HSQC,HMBC and NOESY were used to completely assign the spectra of 1 and 2 for the first time.

Chemical Constituents of Phyllanthus niruri L.

Abstract: Five compounds were isolated from the aerial part whole plants of Phyllanthus niruri L.and identified as corilagin(1),rutin(2),isobubbialine(3),succinic acid(4),and gallic acid(5) on the basis of physical and chemical properties and spectra data.The assignment of NMR signals of compound 3 was revised,and the NMR signals of compound 1 were assigned with 1D and 2D-NMR spectra.

Volatile Components of Essential Oil from Mulberry Variety "Longsang 1" Leaves

Abstract: The mulberry variety "Longsang 1"(Morus alba) was cultivated in Gannan Forest Mulberry Industry Science and Technology Demonstration Field of the Qiqihar City in Heilongjiang Province in Northeastern China. In July,August and September of 2009,the leaves of "Longsang 1" were collected,then the volatile components of the essential oils in the leaves were hydrodistillated and analyzed by gas chromatography-mass spectrometry.The results showed that phytol(alcohols),hexahydrofamesyl acetone(ketones),heptacosane,and pentacosane (hydrocarbons) were the common volatile components of the essential oil.Hexadecanoic acid was dominant in the essential oil from leaves collected in July and August.(E)-β-farnesene,(Z)-β-farnesene,β-bisabolene,trans-αbergamotene and α-curcumene were the main volatile flavor compounds in the essential oil from leaves collected in September.Air-dried mulberry leaves collected in September has relatively high medicinal value due to their higher content of terpenoids in the essential oil.

Advances in the Research of Phytochemistry and Pharmacology of Meconopsis Vig

Abstract: The phytochemical and pharmacological studies on medicinal plants of Meconopsis genus have been reviewed in this article

Abietane diterpenes from Callicarpa pedunculata

Abstract: Five abietane diterpenes were isolated from CHCl3-solved portion of EtOH extraction from Callicarpa pedunculata R.Brown.On the basis of spectroscopic analysis,these compounds were identified as dehydroabietic acid(1),7β-hydroxydehydroabietic acid(2),7α-hydroxydehydroabietic acid(3),7-oxodehydroabietic acid(4),and 6,8,11,13-abietatrien-18-oic acid(5).These compounds were isolated from this plant for the first time.

Progress of Chemical Constituents and Pharmacology of Genus Rubus

Abstract: The chemical constituents and pharmacology of Rubus in the latest 10 years were summarized to supply the scientific basis for further exploitation of Rubus.Flavonoids,terpenoids,tannins and steroids are the main constituents of Rubus.It has wide range of pharmacological effects including antibiosis,antiinflammatory,antitumor,antioxidant,anti-allergic,hepatoprotective and antinociceptive.

Chemical Constituents from Polygonum capitatum

Abstract: Nine compounds were isolated from EtOAc extract of the Polygonum capitatum Buch.-Ham.ex D.Don..Their structures were identified as quercetin 3-O-(4″-O-acetyl)-α-L-rhamnopyranoside(1),quercetin(2),quercitrin(3),myricetrin(4),quercetin 3-rhamnoside 2″-gallate(5),protocatechuic acid(6),daucosterol(7),gallic acid(8) and progallin A(9) on the basis of their physical properties and the spectral data.Compound 1 was isolated from the genus Polygonum for the first time,and 4 was isolated from the plant for the first time.The NMR spectroscopic characteristics of the acetylated rhamnose of flavonoids glycosides were summarized.

Optimization of Extraction of Total Triterpenoids from Submergedly Cultured Antrodia camphorata using Response Surface Methodology

Abstract: The optimum conditions for the extraction of total triterpenoids from submergedly cultured Antrodia camphorata were determined by using response surface methodology(RSM).A three-level Box-Behnken factorial design was used to investigate the effects of three independent variables,namely solvent composition,extraction temperature and liquid-solid ratio on the response,the yield of total triterpenoids.The independent variables were coded at three levels and their actual values were selected on the basis of preliminary experimental results.Results showed that the generated regression models adequately explained the data variation and significantly represented the actual relationship between the independent variables and responses.A solvent composition of 86%,an extraction temperature of 75 ℃ and a liquid-solid ratio of 37 were found to be optimal for triterpenoid extraction from A.camphorata in submerged culture.By means of additional experiments,the adequacy of this model was conformed.

Advances in Antifungal Activity of Plant Essential Oil

Abstract: In this paper,advances on antifungal activity and active ingredients of plant essential oil were summarized,which included 31 plant essential oils and 13 active ingredients.In addition,antifungal mechanisms of essential oil and active ingredients were analysed.

Changes of Three Main Nutrient During Oat Germination

Abstract: Germination of cereals has been used for centuries to soften kernel structure,and in order to utilize oat seed sufficiently and increase nutrient content and availability of oat,one of hull-less oat seed from Shan xi province was analysed for three nutrients and β-glucan content.The results as follows:about 10% less starch and slightly less N was found in malted oat than in unmalted,and lipid content slightly decreased(about 0.1%).Free amino acid,soluble carbohydrate and reducing sugar content increased but β-glucan content decreased remarkably during the germination process.

Simultaneous Quantitative Determination of Viterxin,Quercetin and Quercitrin in Polygonum viviparum in Tibet Plateau by RP-HPLC

Abstract: To develop a sensitive and specific reversed-phase high performance liquid chromatography (RP-HPLC) method for simultaneous determination of three flavoniods in Polygonum viviparum,the HPLC separation was performed on a Kromasil C_18 column(250 mm×4.6 mm,5 μm) and detected with DAD at 360 nm. The gradient elution was carried out with methano-H_2O(containing 0.25% phosphoric acid) as the mobile phase at a flow rate of 1 mL/min.The results indicated that the calibration curves for viterxin,quercetin and quercitrin were linear over the ranges of 8-40 μg/mL,5-50 μg/mL and 5.33-52 μg/mL,their assay recoveries were 100.3%, 98.9% and 100.4%,their intraday repeatability were 0.78%,0.51% and 0.38%,and interday repeatability were 2.6%,1.9% and 2.1%,respectively.This method was simple,accurate and sensitive,so it could be used for the quality control of P.viviparum.

Chemical Constituents of the Style of Zea mays L.

Abstract: Fourteen compounds were isolated from the style of Zea mays L.,and their structures were identified as chrysoeriol 7-O-β-glucopyranoside(1),chrysoeriol-6-C-α-boivinopyranosyl-7-O-β-glucopyranoside(2), chrysoeriol-6-C-β-L-boivinopyranoside(3),L-rhamnose(4),stigmasta-4-en-3β,6β-diol(5),7α-hydroxysitosterol(6), 7β-hydroxysitosterol(7),daucosterol plamitate(8),soya-cerebroside I(9),asergosta-7,22-dien-3β,5α,6β-triol(11), palmitic acid(12),daucosterol(13),and β-sitosterol(14).Compounds 1,4,and 7-12 were obtained from corn silk for the first time.

Phytochemical Investigation of Corydalis yanhusuo

Abstract: Seventeen compounds have been isolated from Corydalis yanhusuo W.T.Wang.Their structures were deduced on the basis of their physicochemical properties and spectroscopic data.These compounds,including eleven alkaloids,two anthraquinones,a triterpenoid and three steroids,were identified as dihydrochelerythrine(1),dehydrocorydaline(2),tetrahydrocolumbamine(3),isocorybulbine(4),corydaline(5),tetrahydrocoptisine(6),jatrorrhizine(7),coptisine(8),emodin(9),physcion(10),3β-hydroxy-olean-11,13(18)-dien-28-oic acid(11),berberine(12),palmatine(13),tetrahydropalmatine(14),stigmasterol(15),β-sitosterol(16),and daucosterol(17).Compounds 9-11 were isolated from genus Corydalis for the first time,and 1 was firstly isolated from this species.

Chemical Constituents of Amomum villosum Lour.

Abstract: Fourteen compounds were isolated from Amomum villosum Lour.Their structures were identified by physical-chemical and spectral analysis as Quercetin(1),Quercitroside(2),Isoquercitroside(3),Vanillic acid (4),3,4-Dihydroxy-benzoic acid(5),β-Sitosterol(6),Daucosterol(7),Stigmasterol(8),Ergosterol(9),ergosta-7,22dien-3β,5α,6β-triol(10),Stearic acid(11),Palmitic acid(12),Typhonoside B(13),Polygonin(14).Among them,Compounds 1,9,10,13,and 14 were isolated from Amomum villosum Lour.for the first time.

Chemical Constituents of the Bark of Anthocephalus chinensis

Abstract: Ten compounds were isolated and purified from the bark of Anthocephalus chinensis by column chromatography on silica gel,MCI and Sephadex LH-20,and their structures were elucidated as 4-carboxy-3-hydroxy-5-methylphenyl 3-methoxy-4-hydroxy-5-methylbenzoate(1),sitosterol-3-O-(6′-O-palmityl)-β-D-glucoside(2),quinovic acid-3-O-α-L-rhamnopyranoside(3),clethric acid(4),hederagenin(5),uncargenin C(6),morolic acid(7),caffeic acid methyl ester(8),cadambine(9),and 3α-dihydrocadambine(10) by modern spectroscopic methods.Compound 1 was a new phenolic compound,and compounds 2-8 were obtained from Anthocephalus plants for the first time.

Metabolites of the Endophytic Fungus Alternaria sp. PR-14 of Paeonia delavayi

Abstract: Six compounds were obtained from the fermentation broth extract of the endophytic fungus Alternaria sp.PR14 of Paeonia delavayi.Their structures were identified to be alternariol(1),djalonensone(2),cerebroside B(3),cerebroside C(4),(2S,3S,4R,2' R)-2-(2'-hydroxytetracosanoylamino) octadecane-1,3,4-triol(5),and mannitol(6) by spectral methods.All the compounds were isolated from the endophytic fungus of P.delavayi for the first time.

Research in Nor-oleanane Triterpenoids

Abstract: The nor-oleanane triterpenoids were reviewed with the distribution in plants,type of structure and pharmacological activity.

Chemical Constituents from the Roots of Ligularia fischeri Turcz.

Abstract: Eleven compounds were isolated from the ethanol extracts of the roots of Ligularia fischeri Turcz.They were identified by chemical and spectral methods as protocatechualdehyde(1),7-hydroxy-chrome(2),caffeic acid(3),ferulic acid(4),1,5-dicaffeyol-quinic acid(5),angelic acid(6),β-sitosterol(7),daucosterol (8),petasin(9),isopetasin(10),and pentatriacontane(11).Among them,phenol constituents 1-5,sesquiterpens 9-10 and lipid 11 were isolated from this plant for the first time.

Flavonoids from the Twigs of the Tibetian Medicine Myricaria germanica

Abstract: Eleven flavonoids were isolated from the 60% aqacetone extract of the air-dried twigs of Myricaria germanica(Tamaricaceae),a famous Tibetian medicineTheir structures were elucidated by spectroscopic analysis and identified as 3,5,4′-trihydroxy-7,3′-dimethoxyflavone(1),3,5,4′-trihydroxy-7-methoxyflavone(2),rhamnetin(3),3,5,7-trihydroxy-4′-methoxyflavone(4),tamarixetin(5),kaempferol(6),quercetin-3-O-β-D-glucoside(7),kaempferol-3-O-β-D-glucuronic acid(8),quercetin-3-O-β-D-glucuronicacid(9),quercetrin(10),and afzelin(11),respectivelyAll compounds were obtained from Mgermanica for the first time,and compounds 4,5,7-11 were obtained from the genus Myricaria for the first time

Chemical Constituents of the Alabastrum of Syringa oblata Lindl.

Abstract: Eight compounds were isolated by silica gel column chromatography and HPLC from ethyl acetate fraction of dried alabastrum of Syringa oblata.The structures were identified by means of physic-chemical and spectral data.They are Syringopicrogenin-B(1),oleandic acid(2),ursolic acid(3),lupanic acid(4),luprol(5),p-hydroxy phenylpropanol(6),p-hydroxy phenylethanol(7),and β-sitosterol(8).Compound 6 is isolated from this plant for the first time.The other compounds are isolated from the alabastrum of S.oblata Lindl.for the first time.

Triterpenoids from the leaves of Cerbera manghas

Abstract: Seven triterpenoids were obtained from the leaves of Cerbera manghas and identified as uvaol(1),(23Z)-9,19-cycloart-25-ene-3β,24-diol(2),euphorbol(3),ursolic acid(4),2α-hydroxyursolic acid(5),3-O-acetyl ursolic acid(6),and α-amyrin(7)Among them,1-6 were isolated from genus Cerbera for the first time

Chemical Constituents of Rhizomes of Ficus tikoua

Abstract: The constituents of 90% methanol extract in rhizomes of Ficus tikoua were isolated and purified by repeated chromatography.On the basis of spectral methods,chemical analysis,and reference to the literature data,seven compounds were identified as hydroxyalpinumisoflavone(1),bergapten(2),β-stigmasterol(3),oleanic acid(4),β-sitosterol(5),vanillic acid(6),2,6-dimethoxy-1,4-benzoquinone(7).Among them,compounds 1,3,6 and 7 were isolated from this plant for the first time.

Chemical Constituents from Lysimachia clethroides

Abstract: Eight known compounds were isolated from the roots of Lysimachia clethroides.Their structures were elucidated as following based on spectral methods as kaempferol-3-O-β-D-glucopyranoside(1),kaempferol-3-O-β-D-(2-O-β-D-glucopyranosyl)glucopyranoside(2),(+)-catechin(3),(-)-epicatechin(4),(+)-gallocatechin(5),(-)-epigallocatechin(6),(E)-2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucopyranoside(7),and 2,3,5,4′-tetrahydroxystilbene-3-O-β-D-glucopyranoside(8).All compounds were isolated for the first time from the roots of this plant.

Chemical Constituents from Inula nervosa Wall.

Abstract: Thirteen compounds were isolated from the 75% ethanol extract of the aerial parts of Inula nervosa Wall..On the basis of spectral data,they were identified as shionone(1),β-sitosterol(2),α-spinasterol(3),ursolic acid(4),daucosterol(5),bigelovin(6),loliolide(7),24S-ethyl-5α-cholesta-7,22E-dien-3α-ol-β-galactopyranoside(8),spinacetin(9),kaempferol(10),α-spinasterol-3-O-β-D-glucoside(11),benzyl-β-D-glucopyranoside(12),and 2-phenylethyl-β-D-glucopyranoside(13).Compounds 1-3 and 6-13 were isolated from this plant for the first time.

Isoflavones in Leaves of Belamcanda chinensis

Abstract: Eight isoflavones were isolated from Leaves of Belamcanda chinensis grown in Hainan Island.Their chemical structures were determined by physical and chemical constants and the data of Spectroscopic identifications.They are iristectorin A(1),iridin (2),tectoridin(3),tectorigenin(4),irisflorentin(5),genistein(6),genistin(7),and Prunetin(8).All these compounds were isolated from the Leaves of Belamcanda chinensis for the first time,Compound 7 and Compound 8 were isolated from Iridaceae for the first time.

Chemical Constituents in Herb of Salvia cavaleriei Lévl.

Abstract: Eight compounds were isolated form the 95% ethanol extract of the whole plants of Salvia cavaleriei Levl.On the basis of physicochemical properties and spectral methods they were identified as tritriacontane(1),myristyl dodecanoate(2),β-sitosterol(3),daucosterol(4),ursolic acid(5),2a,3β-dihydroxyursolic acid(6),betulinic acid(7),rosmarinic acid(8).All of the compounds were isolated from the stems of this plant for the first time.

Chemical Constituents of the Root of Arctium lappa L.

Abstract: Six compounds were isolated and purified from 95% ethanol extract of the root of Arctium lappa L.by chromatographic methods.Among them,five chemical constituents were identified by physico-chemical and spectroscopic analysis as arctiin(1),kaempferol(2),adenosine(3),solasonine(4),and spirosl-3-O-α-L-rhamnopyrannosyl-(1→4)-O-β-D-galactopyranosyl(5).Compounds 2～5 were firstly isolated from the burdock root.