Showing papers in "Virology in 1977"

TL;DR: The finding that with three different paramyxoviruses the biologically active virions possess F1 and F2 polypeptides suggests that this is a general feature of paramyxOViruses, and that the activation of infectivity, cell fusion, and hemolysis is due to a conformational change in the F protein resulting from proteolytic cleavage to form an active complex of two disulfide-linked polypePTide chains.

TL;DR: Host-range mutants of adenovirus type 5 have been selected on human embryo kidney cells transformed by sheared adeno 5 DNA and these mutants grow well on 293 cells, but are restricted on HeLa cells.

TL;DR: The results of pulse and pulse-chase experiments suggest that large, polyprotein precursors are not involved in Sendai virus replication, and that polypeptides are synthesized from monocistronic messenger RNA species.

TL;DR: It is apparent from these investigations that the two types of cRNA are synthesised by the same transcriptase but that the production of unpolyadenylated complete transcripts is dependent upon the synthesis of certain other viral protein(s).

TL;DR: While HPV 1–3 are closely related isolates, HPV 4 represents a new human papilloma virus, profoundly different from the previous ones as far as base composition and antigenicity are concerned.

TL;DR: Comparison of the synthesis and properties of a polypeptides specified by herpes simplex viruses (HSV) 1 and 2 showed the following: the three earliest virus-specific infected cell polyPEptides (ICP) made in HSV-2 infected cells migrated slightly more slowly in polyacrylamide gels than theHSV-1 α polypePTides.

TL;DR: Competition radioimmune assays demonstrated that protein extracts of NP MC29 cells contain inhibitory activity for precipitation of p19 and p27, but not for p15, and Pulse-chase experiments showed that the MC29–110K polypeptide turned over at a rate comparable to that of pr76, but none of the major structural proteins could be detected after the chase.

TL;DR: Observations demonstrate that cleavage is a precondition for the biological activity not only of glycoprotein F but also of Glycoprotein HN, and there is a striking difference between glycoproteins HN0 and F0 with repsect to their susceptibility to proteolytic enzymes.

TL;DR: It is found that immortalization by the B95-8 strain of EBV follows “one-hit” kinetics, and Lipopolysaccharide from Escherichia coli appears to enhance transformation by mechanisms other than stimulation of cellular DNA synthesis.

TL;DR: Quantitative measurements of pulse-chase profiles and results obtained with the inhibitor TPCK and the arginine analog canavanine indicate that the proteolytic cleavage of Pr1a+b is the primary pathway for the formation of intermediate RT precursors Pr RT1, 2, 3.

TL;DR: Findings suggest that a strongly bonded substituent rather than a peculiar DNA conformation accounts for the inaccessible 5′-termini of adenovirus serotypes, which remain inaccessible to phosphorylation after high temperature phosphat enzyme treatment, or after phosphatase treatment of denatured, single-stranded DNA.

TL;DR: Analysis of [3H]thymidine pulse-labeled duplex AAV DNA monomers by restriction endonuclease Hpa II fragments shows a bimodal pattern of label incorporation with maximum incorporation in a fragment 19 base pairs long which maps 36 or 65 base pairs inside both molecular ends.

TL;DR: Viral infection led to a mild inhibition of host RNA synthesis in the AIS C-3 cells but to a marked inhibition in the LT C-7 cells, and both virus-induced CPE and viral RNA synthesis were enhanced at 34° relative to 28°.

TL;DR: Two-dimensional (isoelectric point and molecular weight) analysis of immunoprecipitates from oncornavirus-infected cells demonstrates two major species of the major envelope viral glycoprotein; it appears that the gp80 form is proteolytically cleaved and sialidated at the plasma membrane to give rise to “mature” copies of gp69,71, some of which is disulfide linked to p14.

TL;DR: The finding of phosphorylation of M and NP in cells suggests that this may play a role in virus replication or assembly, and the behavior of viral phosphopolypeptides in different polyacrylamide-gel systems varies depending on the polypeptide.

TL;DR: Restriction endonuclease cleavage site mapping of the herpes simplex virus type I genome shows that the DNA is composed of a large segment of 80 megadaltons linked to a small segment of 16 megad altons.

TL;DR: In this paper, the authors measured SDS-polyacrylamide gel electrophoresis (SDS-PAGE) of the Coronavirus A59 and found that after heating to 100° in SDS, the electrophoretic mobility of these polypeptides is altered and several new forms of lower mobility are produced.

TL;DR: It is concluded that at least some of the less-than-full-length viral rods found in preparations of the common and wheat strains of TMV, as well as those previously reported for the cowpea strain, represent the encapsidation of subgenomic portions of the viral RNA which are engendered during viral replication.

TL;DR: The feasibility of using a simple serological technique for specific identification of each virus has been demonstrated and the viruses' serological properties were compared.

TL;DR: In vitro reactions of the membrane-modified particle are discussed as possible counterparts of the in vivo uncoating phenomenon.

TL;DR: Bean golden yellow mosaic virus (GYMV) DNA sedimented in sucrose density gradients and in an analytical ultracentrifuge as a single component and represents the first reported single-stranded DNA virus from plants.

TL;DR: A new nomenclature is proposed for the three major coronavirus structural proteins: the two envelope glycoproteins, GP23 and GP180/90 are designated E1 and E2, respectively; the inner core protein, VP50, is designated N.

TL;DR: In helper-packaging experiments, repressed tandem prophages were packaged during lytic growth of a heteroimmune helper phage to investigate the relation between chromosome length and efficiency of packaging of the bacteriophage λ chromosome.

TL;DR: The data indicate that the two major oligosaccharide moieties of the glycoprotein have similar structures, each having two or three branches terminating in the sequence sialic acid-galactose- N -acetylglucosamine at the nonreducing end of the oligose chain.

TL;DR: Abundant local ethylene synthesis during TMV infection thus seems to be a causative factor in redirecting plant metabolism during the hypersensitive reaction.

TL;DR: The results demonstrate that the haemagglutinin is a trimer of the HA 1 and HA 2 subunit in all cases.

TL;DR: Analysis of AKR virions on SDS-polyacrylamide gels under nonreducing conditions demonstrates the presence of a glycoprotein of 90,000 molecular weight, [gp90], which is composed of gp70 linked to p15(E) by one or more labile disulfide bonds.

TL;DR: With CMV the amount of RNA 5 associated with virions seems to be a characteristic determined by the interaction of the host plant with the helper virus and not with the RNA 5, which could be an important factor in the genesis of helper-dependent replicating RNAs.

TL;DR: The polypeptides of measles virions from four different sources have been analyzed and compared by polyacrylamide gel electrophoresis in both phosphate buffer and discontinuous Tris buffer systems, finding a similar pattern of six polypePTides found with the four different virus strains.

TL;DR: In this paper, an early intermediate in λ DNA packaging has been identified as a ternary complex containing DNA, proheads, and pA. This intermediate can be converted to full heads and PFU in a second reaction catalyzed by a prohead-deficient, pAdeficient extract of λ-infected cells.