Journal ArticleDOI
2′(3′),5′-Diphosphate des Nucleosids X und N3-alkylierter Uridin–Derivate
Frank Seela,Quynh Hoa Tran Thi +1 more
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In this paper, the synthesis of 2′(3′),5′-diphosphates of the rare nucleoside X (1a) and the N3 - alkylated uridine derivatives 1b, c, and 2b has been accomplished by treatement of the nucleosides with 15 fold excess pyrophosphoryl chloride.Abstract:
2′(3′), 5′-Diphosphates of the Nucleoside X and N3 - Alkylated Uridine Derivatives
The synthesis of 2′(3′),5′-diphosphates of the rare nucleoside X (1a) and the N3 - alkylated uridine derivatives 1b, c, and 2b has been accomplished by treatement of the nucleosides with 15 fold excess pyrophosphoryl chloride.read more
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Book ChapterDOI
5-AZA-7-Deazapurines: Synthesis and Properties of Nucleosides and Oligonucleotides
Frank Seela,Helmut Rosemeyer +1 more
TL;DR: In this article, the synthesis and properties of base-modified purine nucleosides with a nitrogen atom in a bridgehead position were discussed, and the synthesis of 5-aza-7-deazapurines can be performed either by a linear or a convergent route.
References
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Journal ArticleDOI
Synthesis of modified nucleoside 3′,5′-bisphosphates and their incorporation into oligoribonucleotides with T4 RNA ligase
Jorge R. Barrio,Maria Del Carmen G. Barrio,Nelson J. Leonard,Thomas E. England,Olke C. Uhlenbeck +4 more
TL;DR: A simple procedure is described to prepare nucleoside 3'(2'),5'-bisphosphates from the corresponding nucleosides with the use of pyrophosphoryl chloride, which is rapid, gives nearly quantitative yields and, most importantly, can be used for a variety of nucleosided with base and sugar modifications.
Journal ArticleDOI
Equimolar addition of oligoribonucleotides with T4 RNA ligase.
Olke C. Uhlenbeck,Vicki Cameron +1 more
TL;DR: The data suggest that T4 RNA ligase will be a useful enzyme for the synthesis of oligomers of defined sequence.
Journal ArticleDOI
3-(3-Amino-3-carboxypropyl)uridine: a novel modified nucleoside isolated from Escherichia coli phenylalanine transfer ribonucleic acid.
Journal ArticleDOI
Joining of 3'-modified oligonucleotides by T4 RNA ligase. Synthesis of a heptadecanucleotide corresponding to the bases 61--77 from Escherichia coli tRNAfMet.
Eiko Ohtsuka,Satoshi Nishikawa,A. F. Markham,Sachiyo Tanaka,Tetsuo Miyake,Toshiaki Wakabayashi,M. Ikehara,Masahiro Sugiura +7 more
TL;DR: The 3' terminus of C-C-A was modified by introduction of the ethoxymethylidene group to prevent intra- and intermolecular self-joining reactions at the 3' end to yield a heptadecanucleotide.
Journal ArticleDOI
Darstellung und Eigenschaften von N3‐(3‐L‐Amino‐3‐carboxypropyl)uridin‐5′‐monophosphat – einem modifizierten Nucleotid aus Transfer‐RNA
Frank Seela,Friedrich Cramer +1 more
TL;DR: In this paper, a modified nucleotide from transfer RNA was obtained by alkylation of isopropylideneuridine with ethyl α-L-benzamidoγ-bromobutyrate and after removal of the protecting groups from the intermediate 4.