A DNA Fragment of Herpes Simplex 2 and Its Transcription in Human Cervical Cancer Tissue
TLDR
A human cervical tumor, free of detectable infectious herpes simplex 2 virus, contained a fragment comprising 39% of herpes viral DNA, which is considerably less than that transcribed in productively infected cells.Abstract:
A human cervical tumor, free of detectable infectious herpes simplex 2 virus, contained a fragment comprising 39% of herpes viral DNA. Renaturation kinetics indicate that an average of 1 to 3.5 DNA fragments of herpes simplex virus are present per cell, depending on the ploidy of the cells in this particular tumor. Virus-specific sequences were found linked to highly repetitive sequences of host DNA, which reassociated under conditions designed to preclude reassociation of viral sequences. The tumor also contained RNA transcripts complementary to 5% of the viral DNA. The fraction of viral DNA template transcribed in the cervical tumor is considerably less than that transcribed in productively infected cells (50%).read more
Citations
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Infection with herpes-simplex viruses 1 and 2. II.
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Human genital cancer: synergism between two virus infections or synergism between a virus infection and initiating events?
TL;DR: The model reconciles seroepidemiological data linking HSV to human genital cancer with the apparent difficulties in finding HSV DNA by biopsy in genital cancer.
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Simian Virus 40 DNA Sequences in DNA of Healthy Adult Mice Derived from Preimplantation Blastocysts Injected with Viral DNA
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References
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A Polynucleotide Segment Rich in Adenylic Acid in the Rapidly-Labeled Polyribosomal RNA Component of Mouse Sarcoma 180 Ascites Cells
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Properties of hamster embryo fibroblasts transformed in vitro after exposure to ultraviolet-irradiated herpes simplex virus type 2.
Ronald Duff,Fred Rapp +1 more
TL;DR: Herpes simplex virus antigens were detected in the transformed cells by the indirect immunofluorescence technique, indicating the continued involvement of the HSV-2 genome in an oncogenic cell line.
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