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A gas chromatographic method for carbohydrates as alditol-acetates
Leroy G. Borchardt,Carl V. Piper +1 more
- Vol. 53, Iss: 2, pp 257-260
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TLDR
A gas chromatographic procedure is described which permits multiple analyses of monosaccharides in wood pulps to be carried out in a working time of 2-3 hr per sample and the sensitivity is increased significantly.Abstract:
A gas chromatographic procedure is described which permits multiple analyses of monosaccharides in wood pulps to be carried out in a working time of 2-3 hr per sample. A single pulp analysis may be completed in an 8-hr day. For the analysis, the monosaccharides present in a hydrolyzed sample are reduced to the alditols with sodium borohydride. Acetylation with acetic anhydride and sulfuric acid permits this step to be completed in a period of 1 hr. The acetylated mixture is precipitated in ice water and extracted with methlyene chloride for injection into the chromatograph. Precision of the method is comparable to that obtained by paper chromatography and the sensitivity is increased significantly.read more
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Journal ArticleDOI
A gas chromatographic method for the determination of aldose and uronic Acid constituents of plant cell wall polysaccharides.
Thomas M. Jones,Peter Albersheim +1 more
TL;DR: Galacturonic acid is found to be a major component of all the cell wall polysaccharides examined, and this technique has been used to determine the changes in composition of Red Kidney bean (Phaseolus vulgaris) hypocotyl cell walls during growth, and to compare thecell wall poly Saccharide compositions of several parts of bean plants.
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Cellulose–hemicellulose and cellulose–lignin interactions in wood pyrolysis at gasification temperature
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Pyrolysis behaviors of wood and its constituent polymers at gasification temperature
TL;DR: In this article, the behavior of wood at gasification temperature (800°C) was investigated focusing on the behaviors of the wood constituent polymers [cellulose, hemicellulose (glucomannan and xylan) and lignin (milled wood Lignin] and showed that comparatively stable primary tar from wood polysaccharides undergo secondary reactions including carbonization after condensation at the reactor wall with lower temperature than their boiling points.
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Nitrobenzene and Cupric Oxide Oxidations
TL;DR: In this paper, a modified Bischler procedure for conversion of isoeugenol into vanillin was applied, which achieved a yield of about 25% (based on Klason lignin) on oxidation of extractive-free spruce (Picea abies) wood meal with nitrobenzene in 2 M sodium hydroxide solution at 160°C for 3 h. When the wood residue from the oxidation was treated again under the same conditions, about 2% additional vanillin were obtained, in addition to insignificant amounts of guaiacol (6),
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Xyloglucan Endo-transglycosylase (XET) Functions in Gelatinous Layers of Tension Wood Fibers in Poplar—A Glimpse into the Mechanism of the Balancing Act of Trees
Nobuyuki Nishikubo,Tatsuya Awano,Alicja Banasiak,Veronica Bourquin,Farid M. Ibatullin,Ryo Funada,Harry Brumer,Tuula T. Teeri,Takahisa Hayashi,Björn Sundberg,Ewa J. Mellerowicz +10 more
TL;DR: It is proposed that XET activity is essential for G-fiber shrinking by repairing xyloglucan cross-links between G- and S(2)-layers and thus maintaining their contact.