Journal ArticleDOI
Colorimetric Method for Determination of Sugars and Related Substances
TLDR
In this article, a method was developed to determine submicro amounts of sugars and related substances using a phenol-sulfuric acid reaction, which is useful for the determination of the composition of polysaccharides and their methyl derivatives.Citations
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Albumin standards and the measurement of serum albumin with bromcresol green
TL;DR: The results by this method agree very well with those obtained by electrophoresis and salt fractionation and the method is simple, it has excellent precision and the reagents are stable.
Journal ArticleDOI
The involvement of cell-to-cell signals in the development of a bacterial biofilm
David G. Davies,Matthew R. Parsek,Matthew R. Parsek,Matthew R. Parsek,James P. Pearson,James P. Pearson,James P. Pearson,Barbara H. Iglewski,Barbara H. Iglewski,Barbara H. Iglewski,J. W. Costerton,J. W. Costerton,J. W. Costerton,E. P. Greenberg,E. P. Greenberg,E. P. Greenberg +15 more
TL;DR: The involvement of an intercellular signal molecule in the development of Pseudomonas aeruginosa biofilms suggests possible targets to control biofilm growth on catheters, in cystic fibrosis, and in other environments where P. aerug inosaBiofilms are a persistent problem.
Journal ArticleDOI
Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose.
TL;DR: A simple and rapid method for isolating pure mouse IgG1, IgG 2a and IgG2b immunoglobulins in nearly 100% yield is described and all IgG was retained at pH 8.0 and this could not be eluted by washing.
Journal ArticleDOI
Extraction of extracellular polymers from activated sludge using a cation exchange resin
TL;DR: In this article, the extraction of water soluble extracellular polymeric substances (EPS) from activated sludge was investigated, which consisted mainly of protein but also humic compounds, carbohydrates, uronic acids and DNA.
References
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A colorimetric method for the determination of sugars.
TL;DR: Preliminary experiments showed that the anthrone and the α-naphthol sulphonate reagents give good results with pure sugar solutions, but the presence of only traces of solvents such as butanol, phenol and propionic acid used in the chromatographic separation of the sugars rendered them useless.
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Quantitative Determination of Carbohydrates With Dreywood's Anthrone Reagent
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Filter-paper partition chromatography of sugars: 1. General description and application to the qualitative analysis of sugars in apple juice, egg white and foetal blood of sheep. with a note by R. G. Westall.
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Aniline Hydrogen Phthalate as a Spraying Reagent for Chromatography of Sugars
TL;DR: The use of ammoniacal silver nitrate solution as a spraying reagent for revealing the presence of sugars on filter-paper chromatograms has the advantage of general application but has a corresponding disadvantage in reacting with a very wide range of reducing substances other than the sugars, including various impurities commonly present in such solvents as phenol and collidine.