Journal ArticleDOI
A method for studying binding proteins, based upon differential dissociation of small ligand
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This article is published in Biochimica et Biophysica Acta.The article was published on 1969-12-23. It has received 80 citations till now. The article focuses on the topics: Ligand efficiency & Binding domain.read more
Citations
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Journal ArticleDOI
Glucocorticoid receptors: relations between steroid binding and biological effects.
TL;DR: In this article, an allosteric model was proposed to explain the differential effects of inducer, suboptimal inducer and anti-inducer steroids on the conformational changes in the receptor molecules.
Book ChapterDOI
Steroid Hormone Receptors
TL;DR: The dependence of human cancers on hormones, the detection and characterization of steroid receptor interactions, and the physiological and pharmacological implications of steroid hormone receptors are discussed.
Book ChapterDOI
Steroid hormone receptors
Etienne-Emile Baulieu,Michel Atger,Martin Best-Belpomme,Pierre Corvol,Jean-Claude Courvalin,Jan Mester,Edwin Milgrom,Paul Robel,Henri Rochefort,Denise De Catalogne +9 more
TL;DR: In this paper, different aspects of steroid hormone receptors are discussed, and the kinetic parameters of the binding reaction of the uterine receptor-estradiol are also studied, and it is found that when estradiol, estrone, and estriol are compared, the rate of dissociation of receptor steroid complexes is similar for the three steroids.
Journal ArticleDOI
Cytoplasmic and nuclear estradiol and progesterone receptors in human endometrium.
TL;DR: The changes of total estradiol and progesterone receptor sites and of their respective subcellular distributions seem to depend upon the plasma levels of both hormones and to follow the same cause and effect relationships as those demonstrated experimentally in laboratory animals.
Journal ArticleDOI
A rapid assay for binding estradiol to uterine receptor(s)
TL;DR: Data compared with those of equilibrium dialysis experiments show that the values obtained by the two methods for the concentration of the high affinity binding sites are identical within 10%.
References
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Journal ArticleDOI
Some Studies of the Protein-Binding of Steroids and Their Application to the Routine Micro and Ultramicro Measurement of Various Steroids in Body Fluids by Competitive Protein-Binding Radioassay
TL;DR: A 100-fold increase in sensitivity has now been achieved by using tritiated steroids in place of 14C-labeled steroids, by utilizing the CBG's of species other than man, and by using adsorption in Place of dialysis or gel filtration.
Journal ArticleDOI
Measurement of Corticosteroid-Binding Globulin in Man
TL;DR: A method for quantitation of corticosteroid-binding globulin (CBG) has been developed utilizing gel filtration and 4-14C cortisol and increase in binding with decrease in assay temperature was again demonstrated for normal and pregnancy serum utilizing the present method.