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A note on microbial growth on hen egg‐shells

R. G. Board, +2 more
- 01 Jul 1979 - 
- Vol. 20, Iss: 4, pp 413-420
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TLDR
Two strains of cuticle‐digesting pseudomonads were isolated from the surface of hens’ egg‐shells that had been stored in a humid atmosphere at 25 °C and Digestion was due to a protease, the demonstration of which was only achieved in media containing cuticle.
Abstract
1. Two strains of cuticle‐digesting pseudomonads were isolated from the surface of hens’ egg‐shells that had been stored in a humid atmosphere at 25 °C. Digestion was due to a protease, the demonstration of which was only achieved in media containing cuticle. 2. The egg‐shells were colonised by yeasts also, but the growth of these organisms appeared to be dependent upon the pseudomonads for the release of nutrients from the cuticle. 3. The pseudomonads would not grow on cuticle in situ unless the relative humidity was about 100%.

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Citations
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Eggshell factors influencing eggshell penetration and whole egg contamination by different bacteria, including Salmonella enteritidis☆

TL;DR: In this paper, an agar approach was used to assess the bacterial eggshell penetration and the contamination of whole eggs with the selected bacterial strains; the intact shells of agar-filled and whole eggs were inoculated with 10(3) -10(4) cfu of the selected strains.
Journal ArticleDOI

Eggshell penetration by Salmonella : a review

TL;DR: In this paper, the penetratio of Salmonella infection through the shell of an egg was investigated. But the authors focused on the reproduction of the laying hens and not only the reproductive organs of the hens.
Journal ArticleDOI

Trans–shell infection by pathogenic micro–organisms reduces the shelf life of non–incubated bird's eggs: a constraint on the onset of incubation?

TL;DR: It is demonstrated that infection of egg contents is prevalent and occurs within the time required to lay a clutch, and that microbial infection and ambient temperature act independently to reduce egg viability considerably.
Journal Article

The Estimation of the Bactericidal Power of the Blood. With a Note by J. O. IRWIN.

A. A. Miles, +1 more
- 01 Jan 1938 - 
TL;DR: The survival rate of Staph. aureus in a standard volume of denbrinated blood is a reliable quantitative measure of the bactericidal power of blood, and the number of viable organisms in the inoculum and in the blood-bacterium mixture may be estimated with the necessary accuracy by counts of colonies developing from measured volumes of the fluids let fall on to the surface of solid media as mentioned in this paper.

The Microbiology of Eggs

R. G. Board, +1 more
TL;DR: The older literature makes the occasional reference to the vulnerability of an eggshell to bacterial penetration at oviposition as mentioned in this paper, and several surveys have been carried out to investigate the routes of bacterial infection of eggs.
References
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Journal Article

Protein Measurement with the Folin Phenol Reagent

TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI

The estimation of the bactericidal power of the blood.

TL;DR: The survival rate, p, of a measured inoculum of Staph.
Journal ArticleDOI

The effects of moisture and temperature on growth and spore germination in some fungi.

TL;DR: All the isolates were most tolerant of low water activity at temperatures close to the optimum but in some species the optimum and maximum temperatures were higher at reduced water activity.
Journal ArticleDOI

A new ultrasensitive method for the determination of proteolytic activity.

TL;DR: In contrast to other insoluble, non-covalent protein-dye complexes in use for the determination of proteolytic activity, the new chromogenic substrates are not affected by the presence of albumin.
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