FIGURE 3: Inhibition of the bovine tPA-mediated plasminogen activation by PAI-1, PAI-2, and EACA in the presence of fibrinogen fragments. In the case of inhibition by PAI-1 and PAI-2, 2 pmol of tPA was incubated at room temperature for 20 min with 9 pmol PAI-1or PAI-2 before addition to the reaction mixture consisting of 0.1 M Tris pH 7.4, 0.02% Tween 80, 0.27µM Plg, 8.4 µg fibrinogen fragments, and 0.5 mM S-2251 in a total volume of 0.2 mL. EACA was added to the reaction mixture just before initiation of the reaction. The reaction velocity was calculated as the slope of the line in a plot of the absorbance versust2.
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