A Secreted Peptide Growth Factor, Phytosulfokine, Acting as a Stimulatory Factor of Carrot Somatic Embryo Formation
Hidetoshi Hanai,Takuya Matsuno,Manabu Yamamoto,Yoshikatsu Matsubayashi,Toshihiro Kobayashi,Hiroshi Kamada,Youji Sakagami +6 more
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TLDR
Quantification studies using a competition ELISA system employing an anti-PSK-alpha polyclonal antibody showed that PSK production might be related to growth of cells, rather than development of somatic embryos, so the stimulatory effect of PSK on somatic embryo formation might be due to promotion of cell proliferation.Abstract:
Somatic embryogenesis of the carrot (Daucus carota L.) depends on a set of factors, some of which accumulate in culture medium (conditioned medium, CM). When embryogenic cell clusters were transferred to an embryo-inducing medium, addition of CM derived from somatic embryo culture markedly stimulated somatic embryo formation. The active principles were purified using a simple bioassay system and identified to be phytosulfokines (PSKs), sulfated oligopeptide growth factors originally isolated from a CM derived from asparagus {Asparagus officinalis L.) mesophyll culture. Quantification studies using a competition ELISA system employing an anti-PSK-a polyclonal antibody showed that PSK production might be related to growth of cells, rather than development of somatic embryos. Thus the stimulatory effect of PSK on somatic embryo formation might be due to promotion of cell proliferation.read more
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An LRR receptor kinase involved in perception of a peptide plant hormone, phytosulfokine.
TL;DR: Overexpression of this receptor kinase in carrot cells caused enhanced callus growth in response toPSK and a substantial increase in the number of tritium-labeled PSK binding sites, suggesting that PSK and this receptor Kinase act as a ligand-receptor pair.
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NaCl stress causes changes in photosynthetic pigments, proteins, and other metabolic components in the leaves of a true mangrove,Bruguiera parviflora, in hydroponic cultures
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Salt tolerance mechanisms in mangroves: a review
Asish Kumar Parida,Bhavanath Jha +1 more
TL;DR: A general picture of salt tolerance mechanisms of mangroves is given, thus providing a new avenue for development of saltolerance in crop plants through effective breeding strategies and genetic engineering techniques.
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Posttranslationally Modified Small-Peptide Signals in Plants
TL;DR: This review covers 20 years of research into posttranslationally modified small-peptide signals in plants and highlights the importance of the location of the modification group incorporated into the peptides by specific modification enzymes and the peptide chain length defined by the processing enzymes for biological function and receptor interaction.
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Embryo production through somatic embryogenesis can be used to study cell differentiation in plants
Francisco Roberto Quiroz-Figueroa,Rafael Rojas-Herrera,Rosa M. Galaz-Ávalos,Víctor M. Loyola-Vargas +3 more
TL;DR: The focus of this review is on embryo development through somatic embryogenesis and especially the factors affecting cell and embryo differentiation.
References
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Toshio Murashige,Folke Skoog +1 more
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Identification and Isolation of Single Cells that Produce Somatic Embryos at a High Frequency in a Carrot Suspension Culture
Koji Nomura,Atsushi Komamine +1 more
TL;DR: There are at least two phases in the differentiation of embryos from single cells, and the progression of the first phase required exogenous auxin, whereas that of the second phase was inhibited by the same growth regulator.