Journal ArticleDOI
Acid esterase in human lymphoid cells and leukaemic blasts: a marker for T lymphocytes.
TLDR
A non‐specific acid alpha‐naphthyl‐acetate esterase activity was investigated in human lymphoid cell populations from tonsils, blood, thymus and in different leukaemias, and four patterns have emerged.Abstract:
A non-specific acid alpha-naphthyl-acetate esterase activity was investigated in human lymphoid cell populations from tonsils, blood, thymus and in different leukaemias. Four patterns have emerged. (i) The vast majority of T lymphocytes (peripheral blood, tonsils) showed a localized, intense reaction product (‘T-like’). (ii) A thymocyte subpopulation expressed faint, localized enzyme activity (‘Thy-like’) but the majority of thymocytes showed no esterase activity. Some cells from acute lymphoblastic leukaemias with thymocyte surface characteristics had a ‘Thy-like’appearance. (iii) Most B lymphocytes and cells from chronic lymphocytic leukaemias were esterase negative. No activity was seen in mitogen activated peripheral blood T and B lymphoblasts from peripheral blood and in leukaemic blasts from the common form of childhood acute ‘lymphoblastic’leukaemia. (iv) Myeloid cells, including peripheral blood monocytes, and blast cells from acute myeloblastic and chronic granulocytic leukaemias showed an intense, diffuse reaction product (M-like).
It is concluded that the modified esterase technique is a convenient marker for small mature human T lymphocytes in tissue sections and smears. It may also be helpful in the differential diagnosis of acute lymphoblastic leukaemia with T characteristics, the common form of acute ‘lymphoblastic’leukaemia and acute myeloblastic leukaemia.read more
Citations
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Journal ArticleDOI
Primary Sjogren syndrome: clinical and immunopathologic features
TL;DR: Although there is no "cure" for primary Sjogren syndrome patient's symptoms may be significantly improved by measures aimed at prevention of ocular and dental complications and by the recognition of extraglandular features that may be amenable to specific treatment.
Journal ArticleDOI
Morphological and histochemical analyses of two human T-cell subpopulations bearing receptors for IgM or IgG.
Carlo E. Grossi,S.R. Webb,A. Zicca,Peter M. Lydyard,Lorenzo Moretta,Maria Cristina Mingari,Max D. Cooper +6 more
TL;DR: Two subpopulation of circulating human T cells forming rosettes with neuraminidase-treated sheep erythrocytes were purified on the basis of the presence of receptors for IgG (TG cells) or for IgM (TM cells), and were shown to have distinguishing morphological and histochemical characteristics.
Journal ArticleDOI
Identification of alpha-naphthyl butyrate esterase as a plasma membrane ectoenzyme of monocytes and as a discrete intracellular membrane-bounded organelle in lymphocytes.
TL;DR: In lymphocytes, the reaction product was localized in membrane-bounded intracellular organelles, similar to those previously shown to contain phospholipid and called Gall bodies, which indicated that monocytic esterase is an ectoenzyme.
Book ChapterDOI
Cells of the Mononuclear Phagocyte System
TL;DR: At present, ten years later, there is not only additional evidence that in the normal steady state monocytes differentiate to macrophages but also more information about the transition from monocyte tomacrophage during an inflammatory response.
References
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Journal ArticleDOI
Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells.
TL;DR: It is indirectly shown that all or at least a major population of human thymus-derived lymphocytes under certain conditions will form nonimmune rosettes with sheep red blood cells (SRBC), and it is suggested that these ro settes are formed by a rapidly released or metabolized receptor substance on the living cell surface which behaves as a trypsin-sensitive structure produced by the cells themselves.
Journal ArticleDOI
Antisera to acute lymphoblastic leukemia cells.
TL;DR: It is concluded that antisera to ALL may define an antigen which may be restricted in expression to a large subgroup of ALL cases, and which offers considerable diagnostic and prognostic potential.
Journal ArticleDOI
Nonspecific acid esterase activity: a criterion for differentiation of T and B lymphocytes in mouse lymph nodes.
TL;DR: Findings indicate that non‐specific acid esterase activity may serve as a criterion to differentiate peripheral T B lymphocyte in lymphocytes in lymph node sections and smears of mice by light microscopy.
Journal ArticleDOI
Esterase activity in leukocytes demonstrated by the use of naphthol AS-D chloroacetate substrate.
TL;DR: Activity in youngMyeloid cells in non-leukemic bone marrow smears and in the peripheral blood of patients with myeloid leukemia is inhibited less than that in adult segmented neutrophils.
Journal ArticleDOI
Identification, Enumeration, and Isolation of B and T Lymphocytes from Human Peripheral Blood
F. Aiuti,J.-C. Cerottini,R. R. A. Coombs,Max D. Cooper,H. B. Dickler,S. Froland,H. Hugh Fudenberg,M. F. Greaves,H. M. Grey,Henry G. Kunkel,Jacob B. Natvig,J.L. Preuhomme,Enrique Rabellino,R. E. Ritts,D. S. Rowe,Maxime Seligmann,F. P. Siegal,J. Stjernsward,W. D. Terry,J. Wybran +19 more
TL;DR: A group sponsored by the World Health Organization/International Agency for Research on Cancer met recently to evaluate current methods and to make the recommendations which appear below.