Journal ArticleDOI
Adrenergic Stimulation of Cyclic GMP Formation Requires NO‐Dependent Activation of Cytosolic Guanylate Cyclase in Rat Pinealocytes
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TLDR
It can be concluded that the adrenergic stimulation of cGMP formation involves NO synthesis followed by activation of cytosolic guanylate cyclase.Abstract:
Cyclic GMP (cGMP) formation in rat pinealocytes is regulated through a synergistic dual receptor mechanism involving beta- and alpha 1-adrenergic receptors. The effects of NG-monomethyl-L-arginine (NMMA), which inhibits nitric oxide (NO) synthase and NO-mediated activation of cytosolic guanylate cyclase, and methylene blue (MB), which inhibits cytosolic guanylate cyclase, were investigated in an attempt to understand the role of NO in adrenergic cGMP formation. Both NMMA and MB inhibited beta-adrenergic stimulation of cGMP formation as well as alpha 1-adrenergic potentiation of beta-adrenergic stimulation of cGMP formation, whereas they had no effect in unstimulated pinealocytes. The inhibitory action of NMMA was antagonized by addition of L-arginine. On the basis of these findings it can be concluded that the adrenergic stimulation of cGMP formation involves NO synthesis followed by activation of cytosolic guanylate cyclase.read more
Citations
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Generation of the Melatonin Endocrine Message in Mammals: A Review of the Complex Regulation of Melatonin Synthesis by Norepinephrine, Peptides, and Other Pineal Transmitters
TL;DR: The aim of this review is to gather together early and recent data on the effects of the nonadrenergic transmitters on modulation of melatonin synthesis, which reveals the variety of inputs that can be integrated by the pineal gland; what elements are crucial to deliver the very precise timing information to the organism.
Journal ArticleDOI
Pathomechanism of pain-related behavior produced by allografts of intervertebral disc in the rat
Mamoru Kawakami,Tetsuya Tamaki,James Neil Weinstein,Hiroshi Hashizume,Hideto Nishi,Stephen T. Meller +5 more
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Histochemistry of nitric oxide synthase in the nervous system
TL;DR: The histochemistry of nitric oxide synthase may represent a valuable marker for neurochemical, if not structural, alterations observed in neural diseases, regeneration and transplantation.
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Components and connections of the circadian timing system in mammals
TL;DR: This review characterizes the brain sites involved and the pathways responsible for the generation and maintenance of circadian rhythmicity and describes the mechanisms that contribute to the maintenance of this rhythm.
Journal ArticleDOI
NADPH-diaphorase activity of nitric oxide synthase in the olfactory bulb: co-factor specificity and characterization regarding the interrelation to NO formation.
TL;DR: It is concluded that staining of neuronal structures in the presence of NADPH does not necessarily represent NADPH-d activity of NOS, and that glomerular staining in the absence of NAD PH is not due to the NADPH -diaphorase activity of nNOS.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine
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Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor
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Histochemical mapping of nitric oxide synthase in the rat brain.
S.R. Vincent,Hiroshi Kimura +1 more
TL;DR: A widespread role is suggested for the nitric oxide-cyclic guanosine monophosphate system in the nervous system through the direct visualization of the neurons which use this novel signal transduction pathway.