Open AccessJournal Article
An electron microscope and immunohistochemical study of the intracellular location of Toxoplasma tissue cysts within the brains of mice with congenital toxoplasmosis.
T. A. Sims,J. Hay,I. C. Talbot +2 more
TLDR
The wall of intact Toxoplasma tissue cysts within the brains of mice with congenital toxoplasmosis was investigated and contained cystozoites were shown by ultrastructural examination to be surrounded by a layer of microtubules; immunohistochemical staining revealed that this layer contained neurofilament protein.Abstract:
The wall of intact Toxoplasma tissue cysts within the brains of mice with congenital toxoplasmosis was investigated. Smaller cysts were identified within the soma of neurones. With larger cysts, the contained cystozoites were shown by ultrastructural examination to be surrounded by a layer of microtubules; immunohistochemical staining revealed that this layer contained neurofilament protein. Interior to this layer was a much convoluted parasitophorus vacuole membrane; exterior was the host cell membrane. In most cases, synaptic plates were noted on the outer plasma membrane. In no instance were tissue cysts observed either within neuroglial cells or in the absence of host cells. These observations are discussed in relation to the pathogenesis of congenital toxoplasmosis in the brain.read more
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Differences among the three major strains of Toxoplasma gondii and their specific interactions with the infected host.
TL;DR: Toxoplasma gondii is one of the most successful protozoan parasites owing to its ability to manipulate the immune system and establish a chronic infection.
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Diagnosis of toxoplasmosis and typing of Toxoplasma gondii
TL;DR: Conventional non-DNA-based diagnostic methods and DNA-based molecular techniques have provided foundations for further development of more effective and accurate detection of T. gondii infection and will contribute to an improved understanding of the epidemiology, prevention and control of toxoplasmosis.
Journal ArticleDOI
The development and biology of bradyzoites of Toxoplasma gondii.
Louis M. Weiss,Kami Kim +1 more
TL;DR: Assessment of unique bradyzoite-specific structures, such as the cyst wall, should lead to a further understanding of T. gondii biology and lead to an understanding of stress response and signaling pathways.
Journal ArticleDOI
Effects of Toxoplasma gondii Infection on the Brain
TL;DR: The strain (genotype) of T. gondii, genetic factors of the host, and probably the route of infection and the stage of infection of the parasite initiating infection all contribute to the establishment of a balance between the host and the parasite and affect the outcome of the infection.
Journal ArticleDOI
Bioluminescence imaging of Toxoplasma gondii infection in living mice reveals dramatic differences between strains
Jeroen P. J. Saeij,Jon P. Boyle,Michael E. Grigg,Michael E. Grigg,Gustavo Arrizabalaga,John C. Boothroyd +5 more
TL;DR: In vivo growth, dissemination, and reactivation of strains of the protozoan parasite Toxoplasma gondii using a bioluminescence-based imaging system has great potential for identifying important differences in the dissemination and growth of different T. Gondii strains, especially strains with dramatically different disease outcomes.