An Examination for Sperm Capacitation in the Fowl
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None of 10 ova recovered from unmated hens showed signs of development after culture under the same conditions but without incubation with spermatozoa, providing direct evidence that avian spermatoza do not require a period of capacitation within the hen's reproductive tract in order to fertilize ova.Abstract:
The in vitro culture system developed was capable of supporting recently ovulated chicken ova in a viable state for at least 24 hr. Utilizing this system, 8 out of 10 ova obtained from the body cavity or infundibulum of unmated White Leghorn hens, were successfully fertilized in vitro. Microscopic examination of the eight fertile blastodiscs revealed that five had proceeded to the blastula state of development during the 24-hr culture period. The remaining three fertile ova, apparently retarded, had not progressed beyond the early cleavage stage of development. None of 10 ova recovered from unmated hens showed signs of development after culture under the same conditions but without incubation with spermatozoa. These observations afford direct evidence that avian spermatozoa do not require a period of capacitation within the hen’s reproductive tract in order to fertilize ova.read more
Citations
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Journal ArticleDOI
Significance of the need for sperm capacitation before fertilization in eutherian mammals.
TL;DR: coordination of the rate of capacitation appropriate to fertilization in vivo may depend not only on the minimal time ordained for the species, but also on a heterogeneity among subpopulations of spermatozoa within any one sample and the timing of sperm transport to the oviduct.
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A complete culture system for the chick embryo
TL;DR: The fertilized ovum of the chick (Gallus domesticus) is cultured for the total embryonic period by growing it in a series of separate culture systems, the first report of a complete in vitro method for a homoiothermic animal.
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14 – Sperm Competition in Birds: Mechanisms and Function
TL;DR: Although it was once thought unfeasible that females could obtain genetic benefits from multiple mating, resolutions to the so-called “paradox of the lek” now exist.
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Pre-incubation holding of hatching eggs
TL;DR: The time between a hatching egg being produced and set in an incubator can be divided into a laying house period and a storage period, which affects hatching results by the egg hatching period.
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Enigmas of mammalian gamete form and function.
TL;DR: Novel features in the male and the female are represented in a need for sperm capacitation, in an unusual regulation of sperm transport within the oviduct, in the cumulus oophorus and character of the zona pellucida around the small egg, and in a unique configuration of gamete fusion.
References
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Journal ArticleDOI
Distribution of spermatozoa in the oviduct and fertility in domestic birds. ii. transport of spermatozoa in the fowl oviduct.
Journal ArticleDOI
Time relations and their significance in the ovulation and penetration of eggs in rats and rabbits.
C R Austin,A W Braden +1 more