Journal ArticleDOI
Analysis of cytogenetic damage induced in CHO cells by the pyrethroid insecticide fenvalerate.
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Results indicate that fenvalerate in the presence of S9-mix is able significantly to increase the frequency of CA, while in the SCE test this increase occurred both in the Presence and in the absence of a rat liver activation system.Abstract:
A synthetic pyrethroid insecticide, fenvalerate, was tested for its ability to induce chromosome aberrations (CA) and sister chromatid exchanges (SCE) in CHO cells. Fenvalerate was assayed both in the presence and in the absence of a rat liver activation system (S9-mix).
Our results indicate that fenvalerate in the presence of S9-mix is able significantly to increase the frequency of CA, while in the SCE test this increase occurred both in the presence and in the absence of S9-mix.
In addition, fenvalerate affected the cell cycle, causing a decrease in the mitotic index (MI) and in the proliferative rate index (PRI). © 1992 Wiley-Liss, Inc.read more
Citations
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Journal ArticleDOI
Evaluation of the ability of a battery of three in vitro genotoxicity tests to discriminate rodent carcinogens and non-carcinogens: I. Sensitivity, specificity and relative predictivity
TL;DR: It was possible to establish that positive results in all three tests indicate the chemical is greater than three times more likely to be a rodent carcinogen than a non-carcinogen, and a relative predictivity (RP) measure is a useful tool to assess the carcinogenic risk from a positive genotoxicity signal.
Journal ArticleDOI
Induction of micronuclei by five pyrethroid insecticides in whole-blood and isolated human lymphocyte cultures.
TL;DR: The existing information appears to support the idea that pyrethroid insecticides have a weak (cypermethrin, deltamethrin and fenpropathrin) or nule (fenvalerate and permethrin) genotoxic activity in vitro.
Journal ArticleDOI
Evaluation of the ability of a battery of three in vitro genotoxicity tests to discriminate rodent carcinogens and non-carcinogens: III. Appropriate follow-up testing in vivo
David Kirkland,Günter Speit +1 more
TL;DR: The published in vivo UDS, TG and Comet-assay results for 67 carcinogens that were negative or equivocal in the micronucleus test suggest that they both should play a more prominent role in regulatory testing strategies than the UDS test.
Journal ArticleDOI
A core in vitro genotoxicity battery comprising the Ames test plus the in vitro micronucleus test is sufficient to detect rodent carcinogens and in vivo genotoxins.
TL;DR: There is no convincing evidence that any genotoxic rodent carcinogens or in vivo genotoxins would remain undetected in an in vitro test battery consisting of Ames+MNvit.
Toxicological profile for pyrethrins and pyrethroids
TL;DR: This edition supersedes any previously released draft or final profile and is a three-volume set of recommendations for on-scene and hospital medical management of patients exposed during a hazardous materials incident.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Revised methods for the salmonella mutagenicity test
Dorothy M. Maron,Bruce N. Ames +1 more
TL;DR: Two new tester strains, a frameshift strain and a strain carrying an ochre mutation on a multicopy plasmid (TA102), are added to the standard tester set and two substitutions are made in diagnostic mutagens to eliminate MNNG and 9-aminoacridine.
Journal ArticleDOI
New Giemsa method for the differential staining of sister chromatids
Paul Perry,Sheldon Wolff +1 more
TL;DR: If human lymphocytes1 or Chinese hamster2 cells are treated with the base analogue 5-bromodeoxyuridine in the latter part of the S period, Giemsa stained chromosomes exhibit a pattern of condensed and extended segments along their length, allowing the identification of the two chromatids, and the observation of sister chromatid exchanges (SCEs) without recourse to autoradiography.
Journal ArticleDOI
Classification and relationships of induced chromosomal structual changes.
TL;DR: A detailed survey is given of the types and classification of primary structural changes that can be induced in chromosomes and observed at the first metaphase after the initial damage.
Journal ArticleDOI
Cell kinetics and sister-chromatid-exchange frequency in human lymphocytes.
TL;DR: The results show a large variability in the individual SCE base-line frequency, and the importance of the proliferating rate of the culture in determining the SCE frequency is stressed.