Journal ArticleDOI
Androgen metabolism assessment by routine gas chromatography/mass spectrometry profiling of plasma steroids: Part 1, Unconjugated steroids.
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TLDR
Using gas chromatography/mass spectrometry, a method for the simultaneous determination of six plasma steroids is developed, providing a sensitive and specific technique with good accuracy and precision.About:
This article is published in Steroids.The article was published on 1992-07-01. It has received 51 citations till now. The article focuses on the topics: Gas chromatography–mass spectrometry & Mass spectrometry.read more
Citations
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Journal ArticleDOI
The art of measuring steroids: Principles and practice of current hormonal steroid analysis.
TL;DR: This review wants to meet the need for profound information and orientation in the field of steroid analysis, and the pros and cons of the most important methods, such as immunoassays and mass spectrometry based methods will be discussed.
Journal ArticleDOI
Measurement of steroid sex hormones in serum: a comparison of radioimmunoassay and mass spectrometry.
Joanne F. Dorgan,Thomas R. Fears,Robert P. McMahon,Lisa Aronson Friedman,Blossom H. Patterson,Susan F. Greenhut +5 more
TL;DR: Although absolute concentrations may differ for some hormones, radioimmunoassay and mass spectrometry can yield similar estimates of between subject differences in serum concentrations of most steroid sex hormones commonly measured in population studies.
Journal ArticleDOI
Clinical applications of gas chromatography and gas chromatography-mass spectrometry of steroids.
B.G. Wolthers,G.P.B. Kraan +1 more
TL;DR: GC-MS is an important tool for detection of steroid hormone doping and combined with a combustion furnace and an isotope ratio mass spectrometer the misuse of testosterone by athletes can be discovered.
Journal ArticleDOI
Prostate formation in a marsupial is mediated by the testicular androgen 5α-androstane-3α,17β-diol
Geoffrey Shaw,Marilyn B. Renfree,Michael W. Leihy,Cedric H.L. Shackleton,Esther Roitman,Jean D. Wilson +5 more
TL;DR: It is shown that the 3α-reduced derivative of DHT, 5α-androstane-3α,17β-diol (5α-adiol), is formed in testes of tammar wallaby pouch young and is higher in male than in female plasma in this species during early sexual differentiation, suggesting that circulating 5α -adiol is a key hormone in male development.
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Natural sex steroids and their xenobiotic analogs in animal production: Growth, carcass quality, pharmacokinetics, metabolism, mode of action, residues, methods, and epidemiology
TL;DR: In surveying the effects of these compounds in meat-producing animals, it became clear that these drugs increase the growth rate of the treated animals and bring about changes in the carcass that are generally characterized by lower fat content and more lean mass.
References
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Genetic Defects of Steroidogenesis in Premature Pubarche
TL;DR: Premature pubarche is more commonly due to a partial enzyme defect in adrenal steroidogenesis than has been previously recognized.
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Determination of steroid hormones by the use of isotope dilution--mass spectrometry: a definitive method in clinical chemistry.
TL;DR: The technique of ID-MS has been applied to the specific and accurate determination of oestradiol-17β, oestriol, testosterone, progesterone, aldosterone and cortisol in human serum.
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Simple methods for the synthesis of twenty different, highly enriched deuterium labelled steroids, suitable as internal standards for isotope dilution mass spectrometry
TL;DR: In this paper, the stability of the carbon-deuterium bonds has been checked according to the following criteria: storage time in non-protic solvents, incubation in biological samples and derivatization reaction conditions.
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17-Hydroxyprogesterone, androstenedione, and testosterone in normal children and in prepubertal patients with congenital adrenal hyperplasia
TL;DR: To determine maximal plasma levels of androstenedione (A) and testosterone (T) which still can be considered non virilizing in 21-hydroxylase deficiency, and to define “acceptable” OHP levels in CAH, simultaneous determinations of OHP, T, and A in prepubertal patients treated for CAH were performed.
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Synthetic procedures for the preparation of deuterium-labeled analogs of naturally occurring steroids.
TL;DR: Deuteration techniques for the elucidation of stereochemical problems, procedures for the preparation of monodeuterated steroids, and synthesis of deuterated analogs of nonbiologic steroids have therefore not been included in this review.