Patent
Bessel beam plane illumination microscope
TLDR
In this article, a microscope has a light source for generating a light beam having a wavelength, λ, and beamforming optics configured for receiving the light beam and generating a Bessel-like beam that is directed into a sample.Abstract:
A microscope has a light source for generating a light beam having a wavelength, λ, and beam-forming optics configured for receiving the light beam and generating a Bessel-like beam that is directed into a sample. The beam-forming optics include an excitation objective having an axis oriented in a first direction. Imaging optics are configured for receiving light from a position within the sample that is illuminated by the Bessel-like beam and for imaging the received light on a detector. The imaging optics include a detection objective having an axis oriented in a second direction that is non-parallel to the first direction. A detector is configured for detecting signal light received by the imaging optics, and an aperture mask is positionedread more
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Patent
Multiview light-sheet microscopy
TL;DR: In this paper, a method of imaging a live biological specimen includes generating one or more first light sheets; directing the generated first light sheet along respective paths that are parallel with a first illumination axis such that the one or multiple first-light sheets optically interact with at least a portion of the biological specimen in a first image plane; and recording, at each of a plurality of second views, images of fluorescence emitted along a second detection axis.
Patent
Laser machining device and laser machining method
TL;DR: In this article, a laser machining device is provided with a laser light source, a spatial light modulator, a driving unit, a control unit, and a condensing optical system.
Patent
Structured plane illumination microscopy
Robert E. Betzig,Kai Wang +1 more
TL;DR: In this paper, a light source is configured for generating coherent light beam having a wavelength, λ, a light detector, and beam-forming optics configured for receiving the generated light beam and for generating a plurality of substantially parallel Bessel-like beams directed into a sample in a first direction.
Patent
Microscope and method for spim microscopy
TL;DR: In this article, the authors proposed a method for SPIM microscopy, where the sample is moved continuously at a constant speed through the light sheet and a plurality of images are recorded at time intervals, preferably periodic intervals, by means of a detection device during the movement, wherein the image recording duration or exposure time is advantageously dimensioned such that the movement travel of the sample lies within a predefined resolution range of the detection objective.
Patent
Light-pad microscope for high-resolution 3d fluorescence imaging and 2d fluctuation spectroscopy
TL;DR: In this article, an illumination light path for illuminating a sample or object and a viewing light path to view the sample is described, with an illumination region-confining device for selectively illuminating a portion of the substantially two-dimensional object illumination region.
References
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Journal ArticleDOI
Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins
TL;DR: The surpassing of the diffraction barrier in fluorescence microscopy with illumination intensities that are eight orders of magnitude smaller is demonstrated, underscoring the potential to finally achieve molecular resolution in fluorescent microscopy by technical optimization.
Journal ArticleDOI
Selective plane illumination microscopy techniques in developmental biology.
Jan Huisken,Didier Y.R. Stainier +1 more
TL;DR: This review compares the benefits and challenges of light-sheet microscopy with established fluorescence microscopy techniques such as confocal microscopy and discusses the different implementations and applications of this easily adaptable technology.
Journal ArticleDOI
Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution.
E. H. Rego,Lin Shao,J. J. Macklin,Lukman Winoto,G. A. Johansson,Nick Kamps-Hughes,Michael W. Davidson,Mats G. L. Gustafsson +7 more
TL;DR: In this article, the authors demonstrate that reversible photoswitching of a fluorescent protein provides the required nonlinearity at light intensities six orders of magnitude lower than those needed for saturation, and visualize cellular structures by imaging the mammalian nuclear pore and actin cytoskeleton.
Patent
Optical microscopy with phototransformable optical labels
Harald F. Hess,E Betzig Robert +1 more
TL;DR: In this paper, a position-sensitive detector is used to detect intensities of radiation as a function of position on the detector, and an optical system, characterized by a diffraction-limited resolution volume, adapted for imaging light emitted from activated and excited phototransformable optical labels (PTOLs) in a sample onto the position sensitive-detector.
Patent
Transparent material processing with an ultrashort pulse laser
TL;DR: In this paper, a method for welding transparent materials using ultrashort laser pulses was proposed, where the laser is focused near the interface of the materials, generating a high energy fluence at the region to be welded.