Biological Modification of Trichothecene Mycotoxins: Acetylation and Deacetylation of Deoxynivalenols by Fusarium spp
TLDR
Attempts were made to elucidate the acetyl transformation of novel trichothecene mycotoxins, 3a,7a,15-trihydroxy-12,13-epoxytrichotheC-9-en-8-one (deoxynivalenol) and its derivatives, by trichothcene-producing strains of Fusarium nivale, F. roseum, and F. solani.Abstract:
Attempts were made to elucidate the acetyl transformation of novel trichothecene mycotoxins, 3α,7α,15-trihydroxy-12,13-epoxytrichothec-9-en-8-one (deoxynivalenol) and its derivatives, by trichothecene-producing strains of Fusarium nivale, F. roseum, and F. solani. In the peptone-supplemented Czapek-Dox medium, F. roseum converted 3α-acetoxy-7α,15-dihydroxy-12,13-epoxytrichothec-9-en-8-one (3-acetyldeoxynivalenol) to deoxynivalenol. 3-Acetyldeoxynivalenol was also deacetylated by intact mycelia of the three strains in sugar-free Czapek-Dox medium. The growing F. nivale acetylated deoxynivalenol to afford a small amount of 3-acetyldeoxynivalenol. 3α,7α,15-Triacetoxy-12,13-epoxytrichothec-9-en-8-one (deoxynivalenol triacetate) was transformed by the intact mycelium of F. solani into 7α,15-diacetoxy-3α-hydroxy-12,13-epoxytrichothec-9-en-8-one (7,15-diacetyl-deoxynivalenol), which was then deacetylated to give 7α-acetoxy-3α,15-dihydroxy-12,13-epoxytrichothec-9-en-8-one (7-acetyldeoxynivalenol). It was noted that the ester at C-7 was not hydrolyzed by the fungal mycelium.read more
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An adaptive evolutionary shift in Fusarium head blight pathogen populations is driving the rapid spread of more toxigenic Fusarium graminearum in North America
Todd J. Ward,R. M. Clear,Alejandro P. Rooney,Kerry O'Donnell,Don Gaba,Susan K. Patrick,David E. Starkey,Jeannie Gilbert,David M. Geiser,T. W. Nowicki +9 more
TL;DR: Results indicate that selection is driving the rapid spread of an introduced pathogen population that is more toxigenic and potentially more vigorous and has significant implications for food safety and cereal production in North America.
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Molecular and Genetic Studies of Fusarium Trichothecene Biosynthesis : Pathways, Genes, and Evolution
TL;DR: The current understanding of the pathways of biosynthesis, the functions of cloned Tri genes, and the evolution of Tri genes are summarized, focusing on Fusarium species.
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Biological detoxification of fungal toxins and its use in plant breeding, feed and food production.
TL;DR: Current application of research on the biological detoxification of fungal toxins by microorganisms and plants and its practical applications in food and feed production and plant breeding is summarized and expected future developments are outlined.
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Trichothecene 3-o-acetyltransferase protects both the producing organism and transformed yeast from related mycotoxins : cloning and characterization of tri101
Makoto Kimura,Isao Kaneko,Masami Komiyama,Akira Takatsuki,Hiroyuki Koshino,Katsuyoshi Yoneyama,Isamu Yamaguchi +6 more
TL;DR: The results suggested that the introduction of an O-acetyl group at the C-3 position in the biosynthetic pathway works as a resistance mechanism for Fusarium species that produce t-type trichothecenes (trichotheCenes synthesized via the precursor trichotriol).
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Review on biological degradation of mycotoxins
Cheng Ji,Yu Fan,Lihong Zhao +2 more
TL;DR: This review will comprehensively describe the biological degradation of AF, ZEA and DON by microorganisms and specific enzymes isolated from microbial systems that can convert mycotoxins with varied efficiency to non- or less toxic products.
References
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Journal ArticleDOI
Isolation of the Emetic Principle from Fusarium-Infected Corn
TL;DR: A mycotoxin responsible for vomiting in swine has been isolated from Fusarium-contaminated field corn and has been given the trivial name vomitoxin.
Journal ArticleDOI
Deoxynivalenol and Its Monoacetate: New Mycotoxins from Fusarium roseum and Moldy Barley
TL;DR: Deoxynivalenol and its Monoacetate: New Mycotoxins from Fusarium roseum and Moldy Barley as discussed by the authors, and their Monoacetates.
Journal ArticleDOI
Studies on the Toxic Substances in Barley Infected with Fusarium spp
TL;DR: Using the procedure employed for the separation of fusarenon and nivalenol, toxic substances were separated from cereal grains infected with Fusarium in the crop field of Kagawa-ken in 1970 and fusarium roseum or related species were found to be a majority of casual fungi.