Journal ArticleDOI
Bovine in vitro fertilization with frozen-thawed semen
John J. Parrish,J.L. Susko-Parrish,M.L. Leibfried-Rutledge,E.S. Critser,W.H. Eyestone,N. L. First +5 more
TLDR
A procedure to obtain high and repeatable fertilization frequencies for bovine in vitro fertilization (IVF) with frozen-thawed sperm was developed and Heparin was the most important factor in increasing IVF frequencies.About:
This article is published in Theriogenology.The article was published on 1986-04-01. It has received 1165 citations till now. The article focuses on the topics: Human fertilization & Sperm.read more
Citations
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Journal ArticleDOI
Capacitation of bovine sperm by heparin.
TL;DR: These studies demonstrate capacitation of bovine sperm by heparin requires at least a 4-h exposure of sperm toHeparin and suggest that plasma membrane changes prior to an AR can be detected by exposure of bovo sperm to LC.
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High bovine blastocyst development in a static in vitro production system using SOFaa medium supplemented with sodium citrate and myo-inositol with or without serum-proteins.
TL;DR: Under defined culture conditions, addition of citrate and myo-inositol improved blastocyst development to rates comparable to those obtained with serum, BSA or co-culture and that the quality of blastocysts was not affected by the absence of serum or BSA.
Journal ArticleDOI
Oocyte and follicular morphology as determining characteristics for developmental competence in bovine oocytes
TL;DR: Oocytes acquire developmental competence late in the follicular phase, possibly when the first signs of atresia have appeared, and that oocytes with beginning signs of degeneration (class 3) will develop significantly more than all other classes.
Journal ArticleDOI
Effects of cryopreservation procedures on sperm membranes
John E. Parks,James K. Graham +1 more
TL;DR: In this paper, the complexity of the sperm plasma membrane and the many steps in a cryopreservation procedure where membrane perturbations can occur are described, and an attempt has been made to describe the complexities of the SPM and its interaction with its components.
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Effect of bovine sperm separation by either swim-up or Percoll method on success of in vitro fertilization and early embryonic development.
TL;DR: The disadvantages of the Percoll procedure could easily be overcome and the procedure was faster and yielded a six-fold greater recovery of motile spermatozoa than the swim-up method.
References
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Journal ArticleDOI
Analysis of Categorical Data by Linear Models
TL;DR: The special cases of linear functions and logarithmic functions of the 7rin are developed in detail, and some examples of how the general approach can be used to analyze various types of categorical data are presented.
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Normal development following in vitro fertilization in the cow.
Benjamin G. Brackett,Daniel Bousquet,Melinda L. Boice,William J. Donawick,James F. Evans,Michael A. Dressel +5 more
TL;DR: Findings suggested vigorous progressive sperm motility and acrosome integrity to be important features of good sperm samples and the first calf resulting from in vitro fertilization has been found to be completely normal.
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The Effects of Sperm Extracts and Energy Sources on the Motility and Acrosome Reaction of Hamster Spermatozoa in vitro
Barry D. Bavister,Yanagimachi +1 more
TL;DR: The component of the sperm extracts that was responsible for stimulating hamster sperm motility was found to be heat-stable and to have the same elution volume on a Sephadex G-10 column, suggesting that the active components may be similar or possibly the same.
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Factors Affecting Successful In Vitro Fertilization of Bovine Follicular Oocytes
TL;DR: While the presence of cumulus cells was not necessary for penetration of ova, increased frequencies of oocytes with both male and female pronuclei were found when cumuli were present and the use of either follicle-stimulating hormone (FSH) or cAMP resulted in higher fertilization rates.
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A Method for Collecting Motile Spermatozoa from Human Semen
TL;DR: The diluent proved to be a favourable medium for significantly delaying the deterioration of sperm progression over a 24-hour period and yielded sperm suspensions free of seminal plasma and populations of spermatozoa that had increased motility ratings compared with the original semen.