Journal ArticleDOI
High bovine blastocyst development in a static in vitro production system using SOFaa medium supplemented with sodium citrate and myo-inositol with or without serum-proteins.
TLDR
Under defined culture conditions, addition of citrate and myo-inositol improved blastocyst development to rates comparable to those obtained with serum, BSA or co-culture and that the quality of blastocysts was not affected by the absence of serum or BSA.About:
This article is published in Theriogenology.The article was published on 1999-09-01. It has received 718 citations till now. The article focuses on the topics: Chemically defined medium & Blastocyst.read more
Citations
More filters
Journal ArticleDOI
New method for culture of zona-included or zona-free embryos: the Well of the Well (WOW) system.
TL;DR: Apart from its theoretical value in revealing the role of different factors influencing embryo development in vitro, the WOW system may have immediate practical consequences in certain areas of mammalian embryo production.
Journal ArticleDOI
Interspecies Chimerism with Mammalian Pluripotent Stem Cells
Jun Wu,Aida Platero-Luengo,Masahiro Sakurai,Atsushi Sugawara,Maria A. Gil,Takayoshi Yamauchi,Keiichiro Suzuki,Y. S. Bogliotti,Cristina Cuello,Mariana Morales Valencia,Daiji Okumura,Jingping Luo,Marcela Vilarino,Inmaculada Parrilla,Delia Alba Soto,Cristina Martínez,Tomoaki Hishida,Sonia Sánchez-Bautista,M. Llanos Martinez-Martinez,Huili Wang,A. Nohalez,Emi Aizawa,Paloma Martinez-Redondo,Alejandro Ocampo,Pradeep Reddy,Jordi Roca,Elizabeth A. Maga,Concepcion Rodriguez Esteban,W. Travis Berggren,Estrella Nuñez Delicado,Jeronimo Lajara,Isabel Guillen,Pedro Guillen,Josep M. Campistol,Emilio A. Martinez,Pablo J. Ross,Juan Carlos Izpisua Belmonte +36 more
TL;DR: A versatile blastocyst complementation platform based on CRISPR-Cas9-mediated zygote genome editing is established and enrichment of rat PSC-derivatives in several tissues of gene-edited organogenesis-disabled mice is shown.
Journal ArticleDOI
Microbial contamination of embryos and semen during long term banking in liquid nitrogen
TL;DR: Stenotrophomonas maltophilia was the most common contaminant identified from the samples and was further shown to significantly suppress fertilization and embryonic development in vitro and no transmission of bovine viral diarrhea virus and bovinesvirus-1 from infected semen and embryos straws to clean germplasm stored in the same LN tanks or LN was detected.
Journal ArticleDOI
Quantification of Housekeeping Transcript Levels During the Development of Bovine Preimplantation Embryos
Claude Robert,Serge McGraw,Lyne Massicotte,Marco Pravetoni,Fulvio Gandolfi,Marc-André Sirard +5 more
TL;DR: In this paper, RNA levels of eight housekeeping genes were quantified using real-time PCR throughout the pre-plantation period of the bovine embryo to find the most suitable gene to be used as standard.
Journal ArticleDOI
In Vitro Production and Nuclear Transfer Affect Dosage Compensation of the X-Linked Gene Transcripts G6PD, PGK, and Xist in Preimplantation Bovine Embryos
TL;DR: Results of the present study show for the first time that differences in X-chromosome-linked gene transcript levels are related to a perturbed dosage compensation in female and male IVP and female NT-derived embryos.
References
More filters
Journal ArticleDOI
Bovine in vitro fertilization with frozen-thawed semen
John J. Parrish,J.L. Susko-Parrish,M.L. Leibfried-Rutledge,E.S. Critser,W.H. Eyestone,N. L. First +5 more
TL;DR: A procedure to obtain high and repeatable fertilization frequencies for bovine in vitro fertilization (IVF) with frozen-thawed sperm was developed and Heparin was the most important factor in increasing IVF frequencies.
Journal ArticleDOI
Capacitation of Rabbit Spermatozoa in vitro
TL;DR: Initiation, but not completion, of removal or alteration of sperm surface seminal plasma antigenic components was a necessary prerequisite to in vitro insemination for successful achievement of fertilization in vitro.
Journal ArticleDOI
Culture of preimplantation embryos: facts and artifacts
TL;DR: In this review, comparative information on epigenetic regulation of embryo development is discussed, including information on energy substrate and amino acid preferences of embryos and improvements in the design of culture media are discussed, so that higher yields and increased viability of embryos are achieved.
Journal ArticleDOI
Enhanced rates of cleavage and development for sheep zygotes cultured to the blastocyst stage in vitro in the absence of serum and somatic cells: amino acids, vitamins, and culturing embryos in groups stimulate development.
TL;DR: Investigation of sheep zygote development of amino acids, ammonium, vitamins, and culture of embryos in groups in Synthetic Oviduct Fluid medium supplemented with BSA found indirect evidence that ruminant embryos utilize amino acids to a greater extent than do rodent embryos.