Showing papers in "Theriogenology in 1995"

TL;DR: A new method for synchronizing the time of ovulation in cattle using GnRH and PGF2α could have a major impact on managing reproduction in lactating dairy cows, because it allows for AI to occur at a known time of Ovulation and eliminates the need for detection of estrus.

TL;DR: Although there was no difference in pregnancy rate, embryos resulting from co-culture with Buffalo Rat Liver Cells n Menezo's B2 medium developed faster than embryos co-cultured with TCM 199 culture medium.

TL;DR: The disadvantages of the Percoll procedure could easily be overcome and the procedure was faster and yielded a six-fold greater recovery of motile spermatozoa than the swim-up method.

TL;DR: Overall, it appears that treatment with E- 17s and progestogen in combination may be used to effectively control and synchronize follicular wave development and may have important implications in artificial control of ovarian cyclicity and superovulation.

TL;DR: The extreme birth weights of some calves suggest that abnormal prenatal growth occurs in some IVM/IVF-derived bovine embryos and that conditions for co-culture to the blastocyst stage may exacerbate the problem.

TL;DR: A relationship between transient increases in FSH concentrations and emergence of follicular waves throughout the interovulatory interval in Polypay ewes is supported, with the 2 events occurring approximately every 4 d.

TL;DR: Group culture of oocytes from animals that were superovulated and left in the postmortem ovaries for 4 h resulted in exceptionally high rates of embryos after 5 d of IVD, indicating that under the proper conditions, developmental competence is acquired before in vitro maturation.

TL;DR: Egg-yolk Tris extender seems to be superior to the other extenders tested, to preserve dog semen at 4 °C, although differences were not significant for all the parameters.

TL;DR: This study aimed at improving the efficiency of bovine embryo production under defined and undefined conditions by showing that the addition of fetal calf serum to SOF droplets after 20 to 24 h significantly improved blastocyst yields on Day 6 and changing of the media resulted in a slightly improved Day-6 blastocySt yield such that renewal of serum-free medium mimicked the effect of serum addition.

TL;DR: It is concluded that vaginoscopic examination is a good tool for the diagnosis of subacute/chronic endometritis and evaluation of the result of the treatment.

TL;DR: Preliminary study has revealed that physiologic characteristics of bovine ovarian follicles may be reflected by visual data analysis of ultrasound images, and it is anticipated that newly developed techniques of controlling folliculogenesis in cattle may be appropriately modified for diagnostic and interventional procedures in women to augment or suppress fertility.

TL;DR: It is concluded that BSA is required for regular fertilization in vitro and under the authors' experimental conditions, protein-supplementation is not necessary for maturation and development up to the blastocyst stage in vitro.

TL;DR: Small bovine preantral follicles can be cultured for 7 d in the absence of serum and hormones and increase in diameter and react to FSH with enhanced cell proliferation and to estradiol with an increase in cell size.

TL;DR: Treatment with eCG or FSH causes increased estradiol-17β plasma levels, suppressed episodic LH secretion, earlier occurrence of the LH surge and the nucleoli of oocytes from undergoing the normal vacuolization in a certain proportion of donor cattle, and although subtle and hardly measurable, these deviations have a profound effect on the subsequent embryonic developmental capacity.

TL;DR: Results indicate that morphological criteria and follicular fluid progesterone concentration may be used to select bovine cumulus oocyte complexes for high potential of blastocyst development.

TL;DR: Embryo transfer has become the most powerful tool animal breeders have acquired since artificial insemination and satellite nucleus schemes are proposed as more optimal structures of MOET schemes.

TL;DR: Assessment of the development and fates of individual follicles is critical in tailoring ovarian stimulation to individual patients in order to increase the probability of conception.

TL;DR: Bovine embryo sexing has been tested under field conditions in France by officially approved embryo transfer teams of some AI Cooperatives since 1990 and DNA technology has been shown to be highly sensitive, efficient, efficient and accurate and pregnancy rates were not significantly different from that of controls.

TL;DR: It was concluded that, during the early rise in LH secretion, early maturing bull calves had higher circulating LH concentrations than late maturing bulls, and during the weeks preceding and following puberty there was an increase in the quality of semen collected by electroejaculation.

TL;DR: There is a need to direct research attention to pathogen interactions with the zona pellucida to enhance the approved importation of valuable genetic material from free-ranging animals.

TL;DR: Repeated aspiration of viable COC at short intervals is possible, additional FSH-treatment does not increase oocyte yields, and viable blastocysts can be produced from cattle at various reproductive phases irrespective of the reproductive phase is concluded.

TL;DR: It is concluded that highly efficient and accurate PCR-sexing of embryos can be accomplished without the use of micromanipulators, control primers and electrophoresis.

TL;DR: Evaluated the influences of body condition score, reproductive status and breed on the number of ovarian follicles and the quality of oocytes developing in them in 449 cows at slaughter to determine the proportion of normal or abnormal oocytes.

TL;DR: It is suggested that good to excellent agreement exists for classifying Day 7 bovine embryos by stage and by extremes of quality grade (Grades 1 and 4) but not by degree of abnormal morphology (grades 2 and 3).

TL;DR: Comparisons among studies are difficult at best because procedural differences make comparisons among studies and an overall estimate of an expected frequency of endemic fetal loss in dairy cattle difficult atbest.

TL;DR: It is suggested that ovulation rates above 20 per cow do not yield gains in total or transferable embryos, and Vitamin A may improve embryo quality without affecting ovulation rate in superovulated cattle.

TL;DR: This manuscript is a review of the literature concerning studies that compared in vitro-manipulated to in vivo-produced preimplantation embryos from the farm species and apologies in advance for studies which were inadvertently omitted.

TL;DR: A study was conducted to modify the routinely used ovum pick up (OPU) devices to permit use of disposable needles and to simplify the technique and to make it more economical and practical to use.

TL;DR: Results confirm the earlier finding of a good response of goats to pFSH preparations with a high FSH:LH ratio, and suggest that supplementation with approximately 40% pLH may be close to the optimum.

TL;DR: The head of the epididymis was warmer than the testicular parenchyma, but the tail, an important storage site for spermatozoa, was cooler, and caution must be exercised when making inferences about ITT based on measurement of SST.