Journal ArticleDOI
Cell‐Cycle Analysis Using A Monoclonal Antibody to Brdurd
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TLDR
A new method of analysis utilizing a monoclonal antibody to bromodeoxyuridine (BrdUrd) has been developed which makes it possible in most cases to accurately determine phase fractions without resorting to mathematical models.Abstract:
The flow cytometric measurement of DNA distributions of cells has many applications in biomedical research. Phase fractions estimated (calculated) from such distributions are used to study the growth characteristics of various types of cells, particularly when the cells have been exposed to perturbing agents such as chemotherapeutic drugs. For more than 10 years many methods for resolving DNA distributions into the three cell subpopulations (G1, S and G2 + M) have been reported in the literature. A new method of analysis utilizing a monoclonal antibody to bromodeoxyuridine (BrdUrd) has been developed (Gratzner, 1982; Dolbeare et al., 1983) which makes it possible in most cases to accurately determine phase fractions without resorting to mathematical models. The procedure involves the incorporation of BrdUrd by growing (DNA synthesizing) S phase cells, labelling the BrdUrd with a fluorescent monoclonal antibody, and the bivariate measurement of the antibody and of total DNA content, the latter through propidium-iodide staining. The resulting bivariate distributions clearly and simply resolve the three subpopulations. This paper describes the method and illustrates its use in the analysis of various fractions of elutriated exponentially growing Chinese hamster ovary (CHO) cells.read more
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Monoclonal antibody Ki-67: its use in histopathology.
D. C. Brown,K C Gatter +1 more
TL;DR: Monoclonal antibody Ki‐67 is of use in research, providing a means of measuring proliferative activity in a variety of conditions besides malignancy, and may prove of value in monitoring tumour response to established and trial therapies.
Journal ArticleDOI
Adventitial Myofibroblasts Contribute to Neointimal Formation in Injured Porcine Coronary Arteries
TL;DR: This study demonstrates translocation of adventitial fibroblasts to neointima, their phenotypic modulation to myofibro Blasts, and distinct characteristics of my ofibrobl cells within neointimo after severe endoluminal coronary injury, which suggest the significance of vascular fibroBLasts in the process of arterial repair.
Journal ArticleDOI
Review: assessment of cell proliferation in histological material.
P A Hall,D A Levison +1 more
TL;DR: This brief overview will illustrate current ideas about cellular proliferation and its regulation and the advantages and disadvantages of the better known methods for assessing cellular proliferation in histopathological material.
Journal ArticleDOI
Evidence for a general relationship between the induced level of DNA double-strand breakage and cell-killing after X-irradiation of mammalian cells.
TL;DR: X-ray-induced DNA double-strand breakage and lethal lesion induction have been examined using normal and transformed fibroblasts of rodent and human origin and linear relationships were found, suggesting a comparable probability of conversion of DNA dsb into a lethal lesions.
Journal ArticleDOI
Early contribution of pericytes to angiogenic sprouting and tube formation
Ugur Ozerdem,William B. Stallcup +1 more
TL;DR: Transplantation of prostate and mammary tumor fragments into NG2-null mice led to the formation of tumor microvasculature that was invariably NG1-negative, demonstrating that pericytes associated with tumor microvessels are derived from the host rather than from the conversion of tumor cells to a pericyte phenotype.
References
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Journal ArticleDOI
Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication
TL;DR: Monoclonal antibodies specific for 5-bromodeoxyuridine have been produced and applied in detecting low levels of DNA replication on a cell-by-cell basis in vitro and do not cross-react with thymidine.
Journal ArticleDOI
Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine.
TL;DR: Bivariate distributions were measured for bone marrow cells taken from mice that were pulse labeled with BrdUrd at various times after treatment with araC, and the resulting DNA/BrdUrd sequences show the kinetics of recovery from aaC and allow discrimination of the aRAC sterilized cells.
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Mathematical analysis of DNA distributions derived from flow microfluorometry
Phillip N. Dean,James H. Jett +1 more
TL;DR: A computer-based mathematical technique for calculating G I, S, and G2 + M fractions from FMF spectra of DNA distributions from DNA distributions is presented.
Journal ArticleDOI
Cell Microfluorometry: A Method for Rapid Fluorescence Measurement
TL;DR: A high-speed flow system for quantitative determination of fluoresence of cells containing fluorochrome has been developed and results compare well with results of other independent methods.
Journal ArticleDOI
The analysis and interpretation of DNA distributions measured by flow cytometry.
TL;DR: This hypothesis was tested by measuring fluorescence distributions for Chinese hamster ovary and KHT mouse sarcoma cells stained with Hoechst-33258, chromomycin A3, propidium iodide, and acriflavine to show that refinements probably are not necessary because of cell staining and sampling variability.