Journal ArticleDOI
Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication
TLDR
Monoclonal antibodies specific for 5-bromodeoxyuridine have been produced and applied in detecting low levels of DNA replication on a cell-by-cell basis in vitro and do not cross-react with thymidine.Abstract:
Monoclonal antibodies specific for 5-bromodeoxyuridine have been produced and applied in detecting low levels of DNA replication on a cell-by-cell basis in vitro. The immunoglobulin-producing hybridomas were derived from spleen cells of mice immunized with a conjugate of iodouridine and ovalbumin. The cells were fused with the plasmacytoma line SP2/0Ag14. The antibodies produced are highly specific for bromodeoxyuridine and iododeoxyuridine and do not cross-react with thymidine. DNA synthesis in cultured cells exposed to bromodeoxyuridine for as short a time as 6 minutes can be detected easily and rapidly by an immunofluorescent staining method and quantitated by flow cytometry.read more
Citations
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Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality
TL;DR: The bioorthogonal chemical reactions developed to date are described and how they can be used to study biomolecules.
Journal ArticleDOI
Dormancy of micrometastases: balanced proliferation and apoptosis in the presence of angiogenesis suppression.
TL;DR: Data show that metastases remain dormant when tumour cell proliferation is balanced by an equivalent rate of cell death and suggest that angiogenesis inhibitors control metastatic growth by indirectly increasing apoptosis in tumour cells.
Journal ArticleDOI
Adult neurogenesis in the mammalian central nervous system
Guo Li Ming,Hongjun Song +1 more
TL;DR: Advances in the understanding of adult neurogenesis will not only shed light on the basic principles of adult plasticity, but also may lead to strategies for cell replacement therapy after injury or degenerative neurological diseases.
Journal ArticleDOI
Determination of lymphocyte division by flow cytometry
TL;DR: A new technique in which an intracellular fluorescent label is divided equally between daughter cells upon cell division is presented, applicable to in vitro cell division, as well as in vivo division of adoptively transferred cells, and can resolve multiple successive generations using flow cytometry.
Journal ArticleDOI
A chemical method for fast and sensitive detection of DNA synthesis in vivo
TL;DR: The method does not require sample fixation or DNA denaturation and permits good structural preservation, and the small size of the fluorescent azides used for detection results in a high degree of specimen penetration, allowing the staining of whole-mount preparations of large tissue and organ explants.
References
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Journal ArticleDOI
Continuous cultures of fused cells secreting antibody of predefined specificity
G Köhler,C. Milstein +1 more
TL;DR: The derivation of a number of tissue culture cell lines which secrete anti-sheep red blood cell (SRBC) antibodies is described here, made by fusion of a mouse myeloma and mouse spleen cells from an immunised donor.
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A better cell line for making hybridomas secreting specific antibodies
TL;DR: The identification of such a cell line, Sp2/0-Ag14, is reported here the identification of a tumour cell fusion partner that makes no Ig but which can nevertheless be fused with spleen cells to obtain hybrids secreting only the specific antibody.
Journal ArticleDOI
The organization and duplication of chromosomes as revealed by autoradiographic studies using tritium-labeled thymidinee.
J H Taylor,P S Woods,W L Hughes +2 more
TL;DR: Thymidine appeared to be the intermediate required for the experiment, but the labels so far employed have not been satisfactory for microscopic visualization by autoradiographic means.
Journal ArticleDOI
A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells.
TL;DR: By carefully controlling the concentration of PEG and the time of exposure of the cells, it was possible to obtain hybridization frequencies several-hundred-fold higher than those obtained with Sendai virus.
Journal ArticleDOI
Antibodies specific for ribonucleosides and ribonucleotides and their reaction with dna.
TL;DR: These findings show that the major component of bacteriochlorophyll is inert with respect to the foregoing light-induced activities, and that a special kind of reaction center is needed for the photochemistry that leads to photosynthesis.